Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60. min at 60. °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/37013/ http://psasir.upm.edu.my/id/eprint/37013/1/Detection%20of%20Coconut%20cadang-cadang%20viroid%20%28CCCVd%29%20in%20oil%20palm%20by%20reverse%20transcription%20loop-mediated%20isothermal%20amplification%20%28RT-LAMP%29.pdf |
| _version_ | 1848848494209531904 |
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| author | Thanarajoo, Sathis Sri Kong, Lih Ling Kadir, Jugah Lau, Wei Hong Vadamalai, Ganesan |
| author_facet | Thanarajoo, Sathis Sri Kong, Lih Ling Kadir, Jugah Lau, Wei Hong Vadamalai, Ganesan |
| author_sort | Thanarajoo, Sathis Sri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60. min at 60. °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix after the addition of fluorescent reagent, and a laddering pattern band on 2% agarose gel electrophoresis. Conventional RT-PCR with LAMP primers produced amplicons with a sequence identical to the 297-nt CCCVd oil palm variant with the primers being specific for CCCVd and not for other viroids such as PSTVd and CEVd. RT-LAMP was found to be rapid and specific for detecting oil palm CCCVd. |
| first_indexed | 2025-11-15T09:35:23Z |
| format | Article |
| id | upm-37013 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:35:23Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-370132015-12-01T02:26:06Z http://psasir.upm.edu.my/id/eprint/37013/ Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP) Thanarajoo, Sathis Sri Kong, Lih Ling Kadir, Jugah Lau, Wei Hong Vadamalai, Ganesan A reverse transcription loop-mediated isothermal amplification (RT-LAMP) detected Coconut cadang-cadang viroid (CCCVd) within 60. min at 60. °C in total nucleic acid extracted from oil palm leaves infected with CCCVd. Positive reactions showed colour change from orange to green in the reaction mix after the addition of fluorescent reagent, and a laddering pattern band on 2% agarose gel electrophoresis. Conventional RT-PCR with LAMP primers produced amplicons with a sequence identical to the 297-nt CCCVd oil palm variant with the primers being specific for CCCVd and not for other viroids such as PSTVd and CEVd. RT-LAMP was found to be rapid and specific for detecting oil palm CCCVd. Elsevier 2014-06-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/37013/1/Detection%20of%20Coconut%20cadang-cadang%20viroid%20%28CCCVd%29%20in%20oil%20palm%20by%20reverse%20transcription%20loop-mediated%20isothermal%20amplification%20%28RT-LAMP%29.pdf Thanarajoo, Sathis Sri and Kong, Lih Ling and Kadir, Jugah and Lau, Wei Hong and Vadamalai, Ganesan (2014) Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP). Journal of Virological Methods, 202. pp. 19-23. ISSN 0166-0934; ESSN: 1879-0984 10.1016/j.jviromet.2014.02.024 |
| spellingShingle | Thanarajoo, Sathis Sri Kong, Lih Ling Kadir, Jugah Lau, Wei Hong Vadamalai, Ganesan Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP) |
| title | Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP) |
| title_full | Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP) |
| title_fullStr | Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP) |
| title_full_unstemmed | Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP) |
| title_short | Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP) |
| title_sort | detection of coconut cadang-cadang viroid (cccvd) in oil palm by reverse transcription loop-mediated isothermal amplification (rt-lamp) |
| url | http://psasir.upm.edu.my/id/eprint/37013/ http://psasir.upm.edu.my/id/eprint/37013/ http://psasir.upm.edu.my/id/eprint/37013/1/Detection%20of%20Coconut%20cadang-cadang%20viroid%20%28CCCVd%29%20in%20oil%20palm%20by%20reverse%20transcription%20loop-mediated%20isothermal%20amplification%20%28RT-LAMP%29.pdf |