Desiccation tolerance in Phaleria macrocarpa embryonic axes
Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a...
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Penerbit Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36990/ http://psasir.upm.edu.my/id/eprint/36990/1/Desiccation%20tolerance%20in%20Phaleria%20macrocarpa%20embryonic%20axes.pdf |
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| author | Asrity, Suaad Mohamed Ahmed Tsan, Fui Ying Ding, Phebe Syed Aris, Syaripah Ruzaini |
| author_facet | Asrity, Suaad Mohamed Ahmed Tsan, Fui Ying Ding, Phebe Syed Aris, Syaripah Ruzaini |
| author_sort | Asrity, Suaad Mohamed Ahmed |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a horizontal laminar air flow cabinet. They were then desiccated under aseptic condition for periods ranging from 0-8 h. For each desiccation treatment, embryonic axes were drawn randomly for the determination of MC according to ISTA, electrolyte leakage and proliferation on Murashige and Skoog (MS) media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The results obtained from this study indicated that the embryonic axes could tolerate dehydration down to 13.6% with desiccation for 8 h while retaining relatively high viability of 76.7%. This was supported by only gradual increment of electrolyte leakage with the desiccated embryonic axes. All non-desiccated embryonic axes with MC of 52.5% were capable to grow into normal plantlets in vitro but dehydration to MC of 36.0% and further down to 13.6% generally resulted in callus formation with up to 16.7% of the embryonic axes while at least 60.0% of the other embryonic axes were still capable to proliferate as normal plantlets in vitro. |
| first_indexed | 2025-11-15T09:35:17Z |
| format | Article |
| id | upm-36990 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:35:17Z |
| publishDate | 2014 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-369902018-10-16T02:57:30Z http://psasir.upm.edu.my/id/eprint/36990/ Desiccation tolerance in Phaleria macrocarpa embryonic axes Asrity, Suaad Mohamed Ahmed Tsan, Fui Ying Ding, Phebe Syed Aris, Syaripah Ruzaini Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a horizontal laminar air flow cabinet. They were then desiccated under aseptic condition for periods ranging from 0-8 h. For each desiccation treatment, embryonic axes were drawn randomly for the determination of MC according to ISTA, electrolyte leakage and proliferation on Murashige and Skoog (MS) media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The results obtained from this study indicated that the embryonic axes could tolerate dehydration down to 13.6% with desiccation for 8 h while retaining relatively high viability of 76.7%. This was supported by only gradual increment of electrolyte leakage with the desiccated embryonic axes. All non-desiccated embryonic axes with MC of 52.5% were capable to grow into normal plantlets in vitro but dehydration to MC of 36.0% and further down to 13.6% generally resulted in callus formation with up to 16.7% of the embryonic axes while at least 60.0% of the other embryonic axes were still capable to proliferate as normal plantlets in vitro. Penerbit Universiti Kebangsaan Malaysia 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36990/1/Desiccation%20tolerance%20in%20Phaleria%20macrocarpa%20embryonic%20axes.pdf Asrity, Suaad Mohamed Ahmed and Tsan, Fui Ying and Ding, Phebe and Syed Aris, Syaripah Ruzaini (2014) Desiccation tolerance in Phaleria macrocarpa embryonic axes. Sains Malaysiana, 43 (10). pp. 1471-1475. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol43num10_2014/contentsVol43num10_2014.html |
| spellingShingle | Asrity, Suaad Mohamed Ahmed Tsan, Fui Ying Ding, Phebe Syed Aris, Syaripah Ruzaini Desiccation tolerance in Phaleria macrocarpa embryonic axes |
| title | Desiccation tolerance in Phaleria macrocarpa embryonic axes |
| title_full | Desiccation tolerance in Phaleria macrocarpa embryonic axes |
| title_fullStr | Desiccation tolerance in Phaleria macrocarpa embryonic axes |
| title_full_unstemmed | Desiccation tolerance in Phaleria macrocarpa embryonic axes |
| title_short | Desiccation tolerance in Phaleria macrocarpa embryonic axes |
| title_sort | desiccation tolerance in phaleria macrocarpa embryonic axes |
| url | http://psasir.upm.edu.my/id/eprint/36990/ http://psasir.upm.edu.my/id/eprint/36990/ http://psasir.upm.edu.my/id/eprint/36990/1/Desiccation%20tolerance%20in%20Phaleria%20macrocarpa%20embryonic%20axes.pdf |