Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells
Damnacanthal, an anthraquinone compound, is isolated from the roots of Morinda citrifolia L. (noni), which has been used for traditional therapy in several chronic diseases, including cancer. Although noni has long been consumed in Asian and Polynesian countries, the molecular mechanisms by which it...
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Spandidos Publications
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36961/ http://psasir.upm.edu.my/id/eprint/36961/1/Damnacanthal%20is%20a%20potent%20inducer%20of%20apoptosis%20with%20anticancer%20activity%20by%20stimulating%20p53%20and%20p21%20genes%20in%20MCF.pdf |
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| author | Abdul Aziz, Muhammad Yusran Omar, Abdul Rahman Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yong Ismail, Nor Hadiani Ahmad, Syahida Mohamed Alitheen, Noorjahan Banu |
| author_facet | Abdul Aziz, Muhammad Yusran Omar, Abdul Rahman Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yong Ismail, Nor Hadiani Ahmad, Syahida Mohamed Alitheen, Noorjahan Banu |
| author_sort | Abdul Aziz, Muhammad Yusran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Damnacanthal, an anthraquinone compound, is isolated from the roots of Morinda citrifolia L. (noni), which has been used for traditional therapy in several chronic diseases, including cancer. Although noni has long been consumed in Asian and Polynesian countries, the molecular mechanisms by which it exerts several benefits are starting to emerge. In the present study, the effect of damnacanthal on MCF‑7 cell growth regulation was investigated. Treatment of MCF‑7 cells with damnacanthal for 72 h indicated an antiproliferative activity. The MTT method confirmed that damnacanthal inhibited the growth of MCF‑7 cells at the concentration of 8.2 µg/ml for 72 h. In addition, the drug was found to induce cell cycle arrest at the G1 checkpoint in MCF‑7 cells by cell cycle analysis. Damnacanthal induced apoptosis, determined by Annexin V‑fluorescein isothiocyanate/propidium iodide (PI) dual‑labeling, acridine‑orange/PI dyeing and caspase‑7 expression. Furthermore, damnacanthal‑mediated apoptosis involves the sustained activation of p21, leading to the transcription of p53 and the Bax gene. Overall, the present study provided significant evidence demonstrating that p53‑mediated damnacanthal induced apoptosis through the activation of p21 and caspase‑7. |
| first_indexed | 2025-11-15T09:35:09Z |
| format | Article |
| id | upm-36961 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:35:09Z |
| publishDate | 2014 |
| publisher | Spandidos Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-369612015-08-27T06:26:39Z http://psasir.upm.edu.my/id/eprint/36961/ Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells Abdul Aziz, Muhammad Yusran Omar, Abdul Rahman Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yong Ismail, Nor Hadiani Ahmad, Syahida Mohamed Alitheen, Noorjahan Banu Damnacanthal, an anthraquinone compound, is isolated from the roots of Morinda citrifolia L. (noni), which has been used for traditional therapy in several chronic diseases, including cancer. Although noni has long been consumed in Asian and Polynesian countries, the molecular mechanisms by which it exerts several benefits are starting to emerge. In the present study, the effect of damnacanthal on MCF‑7 cell growth regulation was investigated. Treatment of MCF‑7 cells with damnacanthal for 72 h indicated an antiproliferative activity. The MTT method confirmed that damnacanthal inhibited the growth of MCF‑7 cells at the concentration of 8.2 µg/ml for 72 h. In addition, the drug was found to induce cell cycle arrest at the G1 checkpoint in MCF‑7 cells by cell cycle analysis. Damnacanthal induced apoptosis, determined by Annexin V‑fluorescein isothiocyanate/propidium iodide (PI) dual‑labeling, acridine‑orange/PI dyeing and caspase‑7 expression. Furthermore, damnacanthal‑mediated apoptosis involves the sustained activation of p21, leading to the transcription of p53 and the Bax gene. Overall, the present study provided significant evidence demonstrating that p53‑mediated damnacanthal induced apoptosis through the activation of p21 and caspase‑7. Spandidos Publications 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36961/1/Damnacanthal%20is%20a%20potent%20inducer%20of%20apoptosis%20with%20anticancer%20activity%20by%20stimulating%20p53%20and%20p21%20genes%20in%20MCF.pdf Abdul Aziz, Muhammad Yusran and Omar, Abdul Rahman and Subramani, Tamilselvan and Yeap, Swee Keong and Ho, Wan Yong and Ismail, Nor Hadiani and Ahmad, Syahida and Mohamed Alitheen, Noorjahan Banu (2014) Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells. Oncology Letters, 7 (5). pp. 1479-1484. ISSN 1792-1074; ESSN: 1792-1082 http://www.spandidos-publications.com/ol/7/5/1479/abstract 10.3892/ol.2014.1898 |
| spellingShingle | Abdul Aziz, Muhammad Yusran Omar, Abdul Rahman Subramani, Tamilselvan Yeap, Swee Keong Ho, Wan Yong Ismail, Nor Hadiani Ahmad, Syahida Mohamed Alitheen, Noorjahan Banu Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells |
| title | Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells |
| title_full | Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells |
| title_fullStr | Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells |
| title_full_unstemmed | Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells |
| title_short | Damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in MCF-7 breast cancer cells |
| title_sort | damnacanthal is a potent inducer of apoptosis with anticancer activity by stimulating p53 and p21 genes in mcf-7 breast cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/36961/ http://psasir.upm.edu.my/id/eprint/36961/ http://psasir.upm.edu.my/id/eprint/36961/ http://psasir.upm.edu.my/id/eprint/36961/1/Damnacanthal%20is%20a%20potent%20inducer%20of%20apoptosis%20with%20anticancer%20activity%20by%20stimulating%20p53%20and%20p21%20genes%20in%20MCF.pdf |