Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach
Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food, detergent, and pharmaceutical sectors. Crystallization utilizing the counter diffusion method in space was performed with the aim to obtain high re...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36940/ http://psasir.upm.edu.my/id/eprint/36940/1/Crystallographic%20analysis%20of%20ground%20and%20space%20thermostable%20T1%20lipase%20crystal%20obtained%20via%20counter%20diffusion%20method%20approach.pdf |
| _version_ | 1848848472561680384 |
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| author | Mohamad Aris, Sayangku Nor Ariati Leow, Adam Thean Chor Mohamad Ali, Mohd Shukuri Basri, Mahiran Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha |
| author_facet | Mohamad Aris, Sayangku Nor Ariati Leow, Adam Thean Chor Mohamad Ali, Mohd Shukuri Basri, Mahiran Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha |
| author_sort | Mohamad Aris, Sayangku Nor Ariati |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food, detergent, and pharmaceutical sectors. Crystallization utilizing the counter diffusion method in space was performed with the aim to obtain high resolution diffracting crystals with better internal order to improve the accuracy of the structure. Thermostable T1 lipase enzyme has been crystallized in laboratory on earth and also under microgravity condition aboard Progress spacecraft to the ISS in collaboration with JAXA (Japanese Aerospace Exploration Agency). This study is conducted with the aims of improving crystal packing and structure resolution. The diffraction data set for ground grown crystal was collected to 1.3 Å resolution and belonged to monoclinic C2 space group with unit cell parameters a = 117.40 Å, b = 80.95 Å, and c = 99.81 Å, whereas the diffraction data set for space grown crystal was collected to 1.1 Å resolution and belonged to monoclinic C2 space group with unit cell parameters a = 117.31 Å, b = 80.85 Å, and c = 99.81 Å. The major difference between the two crystal growth systems is the lack of convection and sedimentation in microgravity environment resulted in the growth of much higher quality crystals of T1 lipase. |
| first_indexed | 2025-11-15T09:35:03Z |
| format | Article |
| id | upm-36940 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:35:03Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-369402016-09-28T02:47:08Z http://psasir.upm.edu.my/id/eprint/36940/ Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach Mohamad Aris, Sayangku Nor Ariati Leow, Adam Thean Chor Mohamad Ali, Mohd Shukuri Basri, Mahiran Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food, detergent, and pharmaceutical sectors. Crystallization utilizing the counter diffusion method in space was performed with the aim to obtain high resolution diffracting crystals with better internal order to improve the accuracy of the structure. Thermostable T1 lipase enzyme has been crystallized in laboratory on earth and also under microgravity condition aboard Progress spacecraft to the ISS in collaboration with JAXA (Japanese Aerospace Exploration Agency). This study is conducted with the aims of improving crystal packing and structure resolution. The diffraction data set for ground grown crystal was collected to 1.3 Å resolution and belonged to monoclinic C2 space group with unit cell parameters a = 117.40 Å, b = 80.95 Å, and c = 99.81 Å, whereas the diffraction data set for space grown crystal was collected to 1.1 Å resolution and belonged to monoclinic C2 space group with unit cell parameters a = 117.31 Å, b = 80.85 Å, and c = 99.81 Å. The major difference between the two crystal growth systems is the lack of convection and sedimentation in microgravity environment resulted in the growth of much higher quality crystals of T1 lipase. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36940/1/Crystallographic%20analysis%20of%20ground%20and%20space%20thermostable%20T1%20lipase%20crystal%20obtained%20via%20counter%20diffusion%20method%20approach.pdf Mohamad Aris, Sayangku Nor Ariati and Leow, Adam Thean Chor and Mohamad Ali, Mohd Shukuri and Basri, Mahiran and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2014) Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach. BioMed Research International, 2014. art. no. 904381. pp. 1-8. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2014/904381/abs/ 10.1155/2014/904381 |
| spellingShingle | Mohamad Aris, Sayangku Nor Ariati Leow, Adam Thean Chor Mohamad Ali, Mohd Shukuri Basri, Mahiran Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach |
| title | Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach |
| title_full | Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach |
| title_fullStr | Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach |
| title_full_unstemmed | Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach |
| title_short | Crystallographic analysis of ground and space thermostable T1 lipase crystal obtained via counter diffusion method approach |
| title_sort | crystallographic analysis of ground and space thermostable t1 lipase crystal obtained via counter diffusion method approach |
| url | http://psasir.upm.edu.my/id/eprint/36940/ http://psasir.upm.edu.my/id/eprint/36940/ http://psasir.upm.edu.my/id/eprint/36940/ http://psasir.upm.edu.my/id/eprint/36940/1/Crystallographic%20analysis%20of%20ground%20and%20space%20thermostable%20T1%20lipase%20crystal%20obtained%20via%20counter%20diffusion%20method%20approach.pdf |