Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei
Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ∼25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1...
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| Format: | Article |
| Language: | English |
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Wiley-Blackwell Publishing
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36939/ http://psasir.upm.edu.my/id/eprint/36939/1/Crystallization%20and%20preliminary%20crystallographic%20studies%20of%20the%20hypothetical%20protein%20BPSL%201038%20from%20Burkholderia%20pseudomallei.pdf |
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| author | Shaibullah, Sofiyah Mohd Sharif, Nurhikmah Ho, Kok Lian Raih, Mohd Firdaus Nathan, Sheila Mohamed, Rahmah Ng, Chyan Leong |
| author_facet | Shaibullah, Sofiyah Mohd Sharif, Nurhikmah Ho, Kok Lian Raih, Mohd Firdaus Nathan, Sheila Mohamed, Rahmah Ng, Chyan Leong |
| author_sort | Shaibullah, Sofiyah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ∼25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 Å resolution. It belonged to space group C2221, with unit-cell parameters a = 85.36, b = 115.63, c = 46.73 Å. The calculated Matthews coefficient (VM) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%. |
| first_indexed | 2025-11-15T09:35:02Z |
| format | Article |
| id | upm-36939 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:35:02Z |
| publishDate | 2014 |
| publisher | Wiley-Blackwell Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-369392016-01-22T03:07:16Z http://psasir.upm.edu.my/id/eprint/36939/ Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei Shaibullah, Sofiyah Mohd Sharif, Nurhikmah Ho, Kok Lian Raih, Mohd Firdaus Nathan, Sheila Mohamed, Rahmah Ng, Chyan Leong Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ∼25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 Å resolution. It belonged to space group C2221, with unit-cell parameters a = 85.36, b = 115.63, c = 46.73 Å. The calculated Matthews coefficient (VM) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%. Wiley-Blackwell Publishing 2014-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36939/1/Crystallization%20and%20preliminary%20crystallographic%20studies%20of%20the%20hypothetical%20protein%20BPSL%201038%20from%20Burkholderia%20pseudomallei.pdf Shaibullah, Sofiyah and Mohd Sharif, Nurhikmah and Ho, Kok Lian and Raih, Mohd Firdaus and Nathan, Sheila and Mohamed, Rahmah and Ng, Chyan Leong (2014) Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei. Acta Crystallographica. Section F: Structural Biology Communications, 70 (12). pp. 1697-1700. ISSN 2053-230X 10.1107/S2053230X14025278 |
| spellingShingle | Shaibullah, Sofiyah Mohd Sharif, Nurhikmah Ho, Kok Lian Raih, Mohd Firdaus Nathan, Sheila Mohamed, Rahmah Ng, Chyan Leong Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei |
| title | Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei |
| title_full | Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei |
| title_fullStr | Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei |
| title_full_unstemmed | Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei |
| title_short | Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL 1038 from Burkholderia pseudomallei |
| title_sort | crystallization and preliminary crystallographic studies of the hypothetical protein bpsl 1038 from burkholderia pseudomallei |
| url | http://psasir.upm.edu.my/id/eprint/36939/ http://psasir.upm.edu.my/id/eprint/36939/ http://psasir.upm.edu.my/id/eprint/36939/1/Crystallization%20and%20preliminary%20crystallographic%20studies%20of%20the%20hypothetical%20protein%20BPSL%201038%20from%20Burkholderia%20pseudomallei.pdf |