Biochemical and molecular identification of Enterococcus spp. from red pitaya
Red pitaya is a popular fruit worldwide due to its unique appearance and high nutrient contents. Many studies about the nutritional content and the physico-chemical characteristics of red pitaya have been performed but microorganisms that occurred naturally in the fruit remained unknown. Therefore,...
| Main Authors: | , , , , |
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| Format: | Article |
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Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36644/ |
| _version_ | 1848848390184501248 |
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| author | Ong, Yien Yien Tan, Wen Siang Mohamad, Rosfarizan Sieo, Chin Chin Tey, Beng Ti |
| author_facet | Ong, Yien Yien Tan, Wen Siang Mohamad, Rosfarizan Sieo, Chin Chin Tey, Beng Ti |
| author_sort | Ong, Yien Yien |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Red pitaya is a popular fruit worldwide due to its unique appearance and high nutrient contents. Many studies about the nutritional content and the physico-chemical characteristics of red pitaya have been performed but microorganisms that occurred naturally in the fruit remained unknown. Therefore, this study was conducted to characterize the enterococci present in red pitaya. Enterococci were isolated from red pitaya fruit and characterized with biochemical tests, randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) of the 16S rDNA region. A total of 30 bacteria were isolated from red pitaya, of which 20 isolates were confirmed as enterococci, genus Enterococcus. 13 isolates were further confirmed as Enterococcus casseliflavus and 7 were identified as Enterococcus gallinarum. |
| first_indexed | 2025-11-15T09:33:44Z |
| format | Article |
| id | upm-36644 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:33:44Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-366442015-09-04T12:35:14Z http://psasir.upm.edu.my/id/eprint/36644/ Biochemical and molecular identification of Enterococcus spp. from red pitaya Ong, Yien Yien Tan, Wen Siang Mohamad, Rosfarizan Sieo, Chin Chin Tey, Beng Ti Red pitaya is a popular fruit worldwide due to its unique appearance and high nutrient contents. Many studies about the nutritional content and the physico-chemical characteristics of red pitaya have been performed but microorganisms that occurred naturally in the fruit remained unknown. Therefore, this study was conducted to characterize the enterococci present in red pitaya. Enterococci were isolated from red pitaya fruit and characterized with biochemical tests, randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) of the 16S rDNA region. A total of 30 bacteria were isolated from red pitaya, of which 20 isolates were confirmed as enterococci, genus Enterococcus. 13 isolates were further confirmed as Enterococcus casseliflavus and 7 were identified as Enterococcus gallinarum. Elsevier 2014-04 Article PeerReviewed Ong, Yien Yien and Tan, Wen Siang and Mohamad, Rosfarizan and Sieo, Chin Chin and Tey, Beng Ti (2014) Biochemical and molecular identification of Enterococcus spp. from red pitaya. Process Biochemistry, 49 (4). pp. 563-568. ISSN 1359-5113; ESSN: 1873-3298 http://www.sciencedirect.com/science/article/pii/S1359511314000609 10.1016/j.procbio.2014.01.019 |
| spellingShingle | Ong, Yien Yien Tan, Wen Siang Mohamad, Rosfarizan Sieo, Chin Chin Tey, Beng Ti Biochemical and molecular identification of Enterococcus spp. from red pitaya |
| title | Biochemical and molecular identification of Enterococcus spp. from red pitaya |
| title_full | Biochemical and molecular identification of Enterococcus spp. from red pitaya |
| title_fullStr | Biochemical and molecular identification of Enterococcus spp. from red pitaya |
| title_full_unstemmed | Biochemical and molecular identification of Enterococcus spp. from red pitaya |
| title_short | Biochemical and molecular identification of Enterococcus spp. from red pitaya |
| title_sort | biochemical and molecular identification of enterococcus spp. from red pitaya |
| url | http://psasir.upm.edu.my/id/eprint/36644/ http://psasir.upm.edu.my/id/eprint/36644/ http://psasir.upm.edu.my/id/eprint/36644/ |