Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth
Background: Flowers of Bauhinia kockiana were investigated for their anticancer properties. Methods: Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability a...
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| Format: | Article |
| Language: | English |
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African Traditional, Herbal Medicine Supporters Initiative
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36637/ http://psasir.upm.edu.my/id/eprint/36637/1/Bioactivity.pdf |
| _version_ | 1848848388238344192 |
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| author | Chew, Yik Ling Lim, Yau Yan Stanlas, Johnson Ee, Gwendoline Cheng Lian Goh, Joo Kheng |
| author_facet | Chew, Yik Ling Lim, Yau Yan Stanlas, Johnson Ee, Gwendoline Cheng Lian Goh, Joo Kheng |
| author_sort | Chew, Yik Ling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Flowers of Bauhinia kockiana were investigated for their anticancer properties. Methods: Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive functional group of the phenolic compounds. Results: The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI50 30 - 86 µM), but were inactive towards DU145 prostate cancer cell (GI50 > 100 µM). Conclusion: It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer activity. Also, the carboxyl group of compound 1 was also important in anticancer activity. Examination of the PC-3 cells treated with compound 1 using fluorescence microscopy showed that PC-3 cells were killed by apoptosis. |
| first_indexed | 2025-11-15T09:33:42Z |
| format | Article |
| id | upm-36637 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:33:42Z |
| publishDate | 2014 |
| publisher | African Traditional, Herbal Medicine Supporters Initiative |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-366372016-11-21T03:55:24Z http://psasir.upm.edu.my/id/eprint/36637/ Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth Chew, Yik Ling Lim, Yau Yan Stanlas, Johnson Ee, Gwendoline Cheng Lian Goh, Joo Kheng Background: Flowers of Bauhinia kockiana were investigated for their anticancer properties. Methods: Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive functional group of the phenolic compounds. Results: The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI50 30 - 86 µM), but were inactive towards DU145 prostate cancer cell (GI50 > 100 µM). Conclusion: It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer activity. Also, the carboxyl group of compound 1 was also important in anticancer activity. Examination of the PC-3 cells treated with compound 1 using fluorescence microscopy showed that PC-3 cells were killed by apoptosis. African Traditional, Herbal Medicine Supporters Initiative 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36637/1/Bioactivity.pdf Chew, Yik Ling and Lim, Yau Yan and Stanlas, Johnson and Ee, Gwendoline Cheng Lian and Goh, Joo Kheng (2014) Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth. African Journal of Traditional, Complementary and Alternative Medicines, 11 (3). pp. 291-299. ISSN 0189-6016; ESSN: 2505-0044 http://journals.sfu.ca/africanem/index.php/ajtcam/article/view/2007 |
| spellingShingle | Chew, Yik Ling Lim, Yau Yan Stanlas, Johnson Ee, Gwendoline Cheng Lian Goh, Joo Kheng Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth |
| title | Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth |
| title_full | Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth |
| title_fullStr | Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth |
| title_full_unstemmed | Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth |
| title_short | Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth |
| title_sort | bioactivity-guided isolation of anticancer agents from bauhinia kockiana korth |
| url | http://psasir.upm.edu.my/id/eprint/36637/ http://psasir.upm.edu.my/id/eprint/36637/ http://psasir.upm.edu.my/id/eprint/36637/1/Bioactivity.pdf |