Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection
In this work we assess the potential of acetylcholinesterase (AChE) from Channa micropeltes (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from C. micropeltes brain tissues using affinity chromatography gel (procainamide–...
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| Format: | Article |
| Language: | English |
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Friends Science Publishers
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36551/ http://psasir.upm.edu.my/id/eprint/36551/1/Assessment%20of%20acetylcholinesterase%20from%20Channa%20micropeltes%20as%20a%20source%20of%20enzyme%20for%20insecticides%20detection.pdf |
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| author | Sharif, Mohd Sha Aidil Halmi, Mohd. Izuan Effendi Amir Hamzah, Amir Syahir Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus |
| author_facet | Sharif, Mohd Sha Aidil Halmi, Mohd. Izuan Effendi Amir Hamzah, Amir Syahir Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus |
| author_sort | Sharif, Mohd Sha Aidil |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this work we assess the potential of acetylcholinesterase (AChE) from Channa micropeltes (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from C. micropeltes brain tissues using affinity chromatography gel (procainamide–Sephacryl S-1000) showed that the partially purified AChE was most active on acetylthiocholine (ATC) but had low activities on propionylthiocholine (PTC) and butyrylthiocholine (BTC), indicating that the partially purified fraction was predominantly AChE. Soluble AChE was partially purified 9.27-fold with a 91.12% yield. The partially purified AChE displayed the highest activity on ATC at pH 7 and at 30oC using 0.1 M Tris buffer. The enzyme exhibited Michaelis-Menten kinetic constants, Km, for ATC, BTC and PTC at 36, 77 and 250 μM, respectively, and the maximum velocities, Vmax, were 18.75, 0.12 and 0.05 μmol/min/mg protein, respectively. Moreover, the AChE from C. micropeltes presented comparable sensitivity to carbamates and organophosphates insecticides than that from Electrophorus
electricus by comparing half maximal inhibitory concentration values, therefore the enzyme is a valuable source for insecticides detection in Malaysian waters at lower cost. |
| first_indexed | 2025-11-15T09:33:19Z |
| format | Article |
| id | upm-36551 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:33:19Z |
| publishDate | 2014 |
| publisher | Friends Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-365512016-01-26T07:55:56Z http://psasir.upm.edu.my/id/eprint/36551/ Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection Sharif, Mohd Sha Aidil Halmi, Mohd. Izuan Effendi Amir Hamzah, Amir Syahir Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus In this work we assess the potential of acetylcholinesterase (AChE) from Channa micropeltes (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from C. micropeltes brain tissues using affinity chromatography gel (procainamide–Sephacryl S-1000) showed that the partially purified AChE was most active on acetylthiocholine (ATC) but had low activities on propionylthiocholine (PTC) and butyrylthiocholine (BTC), indicating that the partially purified fraction was predominantly AChE. Soluble AChE was partially purified 9.27-fold with a 91.12% yield. The partially purified AChE displayed the highest activity on ATC at pH 7 and at 30oC using 0.1 M Tris buffer. The enzyme exhibited Michaelis-Menten kinetic constants, Km, for ATC, BTC and PTC at 36, 77 and 250 μM, respectively, and the maximum velocities, Vmax, were 18.75, 0.12 and 0.05 μmol/min/mg protein, respectively. Moreover, the AChE from C. micropeltes presented comparable sensitivity to carbamates and organophosphates insecticides than that from Electrophorus electricus by comparing half maximal inhibitory concentration values, therefore the enzyme is a valuable source for insecticides detection in Malaysian waters at lower cost. Friends Science Publishers 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36551/1/Assessment%20of%20acetylcholinesterase%20from%20Channa%20micropeltes%20as%20a%20source%20of%20enzyme%20for%20insecticides%20detection.pdf Sharif, Mohd Sha Aidil and Halmi, Mohd. Izuan Effendi and Amir Hamzah, Amir Syahir and Wan Johari, Wan Lutfi and Abd. Shukor, Mohd. Yunus (2014) Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection. International Journal of Agriculture and Biology, 16 (2). pp. 389-394. ISSN 1560-8530; ESSN: 1814-9596 http://www.fspublishers.org/Issue.php?categoryID=123 |
| spellingShingle | Sharif, Mohd Sha Aidil Halmi, Mohd. Izuan Effendi Amir Hamzah, Amir Syahir Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection |
| title | Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection |
| title_full | Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection |
| title_fullStr | Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection |
| title_full_unstemmed | Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection |
| title_short | Assessment of acetylcholinesterase from Channa micropeltes as a source of enzyme for insecticides detection |
| title_sort | assessment of acetylcholinesterase from channa micropeltes as a source of enzyme for insecticides detection |
| url | http://psasir.upm.edu.my/id/eprint/36551/ http://psasir.upm.edu.my/id/eprint/36551/ http://psasir.upm.edu.my/id/eprint/36551/1/Assessment%20of%20acetylcholinesterase%20from%20Channa%20micropeltes%20as%20a%20source%20of%20enzyme%20for%20insecticides%20detection.pdf |