Purification and characterisation of ,B-l,3-giucanase from trichodenna harzianum BIO 10671
B-1,3-glucanase enzyme from culture filtrate of Trichoderma harzianum BIO 10671 was successively purified by precipitation with 80%acetonefollowed by anion-exchange chromatography on Neobar AQ and chromatofocusing on a Mono P HR 5/20 column. Two {3-1,3-glucanases of 32kDa and 66kDa were purified to...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3653/ http://psasir.upm.edu.my/id/eprint/3653/1/Purification_and_Characterisation_of_%2CB-l%2C3-giucanase_from.pdf |
| _version_ | 1848839589175754752 |
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| author | Mustafa, Muskhazli Ramli, Salfarina Ithnin, Nalisha Tohfah, Nor Farizan |
| author_facet | Mustafa, Muskhazli Ramli, Salfarina Ithnin, Nalisha Tohfah, Nor Farizan |
| author_sort | Mustafa, Muskhazli |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | B-1,3-glucanase enzyme from culture filtrate of Trichoderma harzianum BIO 10671 was successively purified by precipitation with 80%acetonefollowed by anion-exchange chromatography on Neobar AQ and chromatofocusing on a Mono P HR 5/20 column. Two {3-1,3-glucanases of 32kDa and 66kDa were purified to homogeneity as judged by SDS-PAGE. The pH aptimum far the enzymes activity was pH 4-5 and maximum activity was obtained at 4YG. Enzyme activity was slightly inhibited by 20-45% in its activity by 20mM of Zn2+, Ca2+, Co2+, Mi+, Cu2+, Mn2+and Fe2+. The highest f3-1,3-glucanase hydrolysis activity was obtained on laminarin due to the
similarity on f3-glucosidic bonds and followed on pustulan, glucan and cellulose, respectively. |
| first_indexed | 2025-11-15T07:13:51Z |
| format | Article |
| id | upm-3653 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:13:51Z |
| publishDate | 2005 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-36532015-09-15T01:10:04Z http://psasir.upm.edu.my/id/eprint/3653/ Purification and characterisation of ,B-l,3-giucanase from trichodenna harzianum BIO 10671 Mustafa, Muskhazli Ramli, Salfarina Ithnin, Nalisha Tohfah, Nor Farizan B-1,3-glucanase enzyme from culture filtrate of Trichoderma harzianum BIO 10671 was successively purified by precipitation with 80%acetonefollowed by anion-exchange chromatography on Neobar AQ and chromatofocusing on a Mono P HR 5/20 column. Two {3-1,3-glucanases of 32kDa and 66kDa were purified to homogeneity as judged by SDS-PAGE. The pH aptimum far the enzymes activity was pH 4-5 and maximum activity was obtained at 4YG. Enzyme activity was slightly inhibited by 20-45% in its activity by 20mM of Zn2+, Ca2+, Co2+, Mi+, Cu2+, Mn2+and Fe2+. The highest f3-1,3-glucanase hydrolysis activity was obtained on laminarin due to the similarity on f3-glucosidic bonds and followed on pustulan, glucan and cellulose, respectively. Universiti Putra Malaysia Press 2005 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3653/1/Purification_and_Characterisation_of_%2CB-l%2C3-giucanase_from.pdf Mustafa, Muskhazli and Ramli, Salfarina and Ithnin, Nalisha and Tohfah, Nor Farizan (2005) Purification and characterisation of ,B-l,3-giucanase from trichodenna harzianum BIO 10671. Pertanika Journal of Tropical Agricultural Science, 28 (1). pp. 23-31. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(23-31).pdf |
| spellingShingle | Mustafa, Muskhazli Ramli, Salfarina Ithnin, Nalisha Tohfah, Nor Farizan Purification and characterisation of ,B-l,3-giucanase from trichodenna harzianum BIO 10671 |
| title | Purification and characterisation of ,B-l,3-giucanase from
trichodenna harzianum BIO 10671 |
| title_full | Purification and characterisation of ,B-l,3-giucanase from
trichodenna harzianum BIO 10671 |
| title_fullStr | Purification and characterisation of ,B-l,3-giucanase from
trichodenna harzianum BIO 10671 |
| title_full_unstemmed | Purification and characterisation of ,B-l,3-giucanase from
trichodenna harzianum BIO 10671 |
| title_short | Purification and characterisation of ,B-l,3-giucanase from
trichodenna harzianum BIO 10671 |
| title_sort | purification and characterisation of ,b-l,3-giucanase from
trichodenna harzianum bio 10671 |
| url | http://psasir.upm.edu.my/id/eprint/3653/ http://psasir.upm.edu.my/id/eprint/3653/ http://psasir.upm.edu.my/id/eprint/3653/1/Purification_and_Characterisation_of_%2CB-l%2C3-giucanase_from.pdf |