Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application
Recent trends in anti-aging research projected the use of antioxidant compound derived from botanical products. Phenolic acids, flavonoids and high molecular weight polyphenols are some of antioxidants which are able to protect human skin against the harmful effects of ultraviolet irradiation, i.e....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Omics Publishing Group
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36529/ http://psasir.upm.edu.my/id/eprint/36529/1/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.pdf |
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| author | Abdul Wahab, Norliza Abdul Rahman, Russly Ismail, Amin Mustafa, Shuhaimi Hashim, Puziah |
| author_facet | Abdul Wahab, Norliza Abdul Rahman, Russly Ismail, Amin Mustafa, Shuhaimi Hashim, Puziah |
| author_sort | Abdul Wahab, Norliza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Recent trends in anti-aging research projected the use of antioxidant compound derived from botanical products. Phenolic acids, flavonoids and high molecular weight polyphenols are some of antioxidants which are able to protect human skin against the harmful effects of ultraviolet irradiation, i.e. photoaging and skin cancer development. Various studies have demonstrated cocoa beans to contain polyphenols and possess health promoting effects mainly on antiradical property. In this study, the HPLC/DAD quantification of (–)-epicatechin from PBC123 and PBC140 Malaysian unfermented cocoa bean extracts (CBEs) were 121.01 and 118.09 mg/g DW, whereas concentration of (+)-catechin were 6.65 and 6.53 mg/g DW, respectively with no significant differences (p>0.05). In term of antioxidant capacity, Ferric reducing/antioxidant power of the respective clones were assayed at 822.10 and 795.99 mM FeSO4/g DW. Inhibition of proteinases expression induces by reactive oxygen species were exhibited in the anti-collagenase and anti-elastase assays. The anti-collagenase activity of PBC123 and PBC140 were 62.99% and 59.96% whereas anti-elastase has been measured at 36.60% and 15.75%, respectively. Positive and high correlation were observed within epicatechin content (1), FRAP (2) and anti-collagenase (3) with significant relationships for both PBC123 and PBC140 (r12=0.901, r13=0.768 and r23=0.908). A statistical One-Way ANOVA showed that there was no significant difference obtained between PBC123 and PBC140 in terms of epicatechin, FRAP and anti-collagenase assays, however, significant difference was observed from anti-elastase assessment (p<0.05).These results indicate unfermented PBC123 clone as a potential source of natural ingredient in a cosmetic industry. |
| first_indexed | 2025-11-15T09:33:14Z |
| format | Article |
| id | upm-36529 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:33:14Z |
| publishDate | 2014 |
| publisher | Omics Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-365292016-04-06T04:39:38Z http://psasir.upm.edu.my/id/eprint/36529/ Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application Abdul Wahab, Norliza Abdul Rahman, Russly Ismail, Amin Mustafa, Shuhaimi Hashim, Puziah Recent trends in anti-aging research projected the use of antioxidant compound derived from botanical products. Phenolic acids, flavonoids and high molecular weight polyphenols are some of antioxidants which are able to protect human skin against the harmful effects of ultraviolet irradiation, i.e. photoaging and skin cancer development. Various studies have demonstrated cocoa beans to contain polyphenols and possess health promoting effects mainly on antiradical property. In this study, the HPLC/DAD quantification of (–)-epicatechin from PBC123 and PBC140 Malaysian unfermented cocoa bean extracts (CBEs) were 121.01 and 118.09 mg/g DW, whereas concentration of (+)-catechin were 6.65 and 6.53 mg/g DW, respectively with no significant differences (p>0.05). In term of antioxidant capacity, Ferric reducing/antioxidant power of the respective clones were assayed at 822.10 and 795.99 mM FeSO4/g DW. Inhibition of proteinases expression induces by reactive oxygen species were exhibited in the anti-collagenase and anti-elastase assays. The anti-collagenase activity of PBC123 and PBC140 were 62.99% and 59.96% whereas anti-elastase has been measured at 36.60% and 15.75%, respectively. Positive and high correlation were observed within epicatechin content (1), FRAP (2) and anti-collagenase (3) with significant relationships for both PBC123 and PBC140 (r12=0.901, r13=0.768 and r23=0.908). A statistical One-Way ANOVA showed that there was no significant difference obtained between PBC123 and PBC140 in terms of epicatechin, FRAP and anti-collagenase assays, however, significant difference was observed from anti-elastase assessment (p<0.05).These results indicate unfermented PBC123 clone as a potential source of natural ingredient in a cosmetic industry. Omics Publishing Group 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36529/1/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.pdf Abdul Wahab, Norliza and Abdul Rahman, Russly and Ismail, Amin and Mustafa, Shuhaimi and Hashim, Puziah (2014) Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application. Natural Products Chemistry & Research, 2 (3). art. no. 1000132. pp. 1-6. ISSN 2329-6836 http://www.esciencecentral.org/journals/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.php?aid=25764 10.4172/2329-6836.1000132 |
| spellingShingle | Abdul Wahab, Norliza Abdul Rahman, Russly Ismail, Amin Mustafa, Shuhaimi Hashim, Puziah Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application |
| title | Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application |
| title_full | Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application |
| title_fullStr | Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application |
| title_full_unstemmed | Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application |
| title_short | Assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of Malaysian unfermented cocoa bean for cosmetic application |
| title_sort | assessment of antioxidant capacity, anti-collagenase and anti-elastase assays of malaysian unfermented cocoa bean for cosmetic application |
| url | http://psasir.upm.edu.my/id/eprint/36529/ http://psasir.upm.edu.my/id/eprint/36529/ http://psasir.upm.edu.my/id/eprint/36529/ http://psasir.upm.edu.my/id/eprint/36529/1/assessment-of-antioxidant-capacity-anticollagenase-and-antielastase-assays-of-malaysian-unfermented-cocoa-bean-for-cosmetic-application-2329-6836.1000132.pdf |