Application of different feeding strategies in fed batch culture for pullulanase production using sago starch
The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2 L bioreactor. In batch culture, 0.0803 U/mL of pullulanase activity with specific activity of 0.0213 U/mg was produced by controlling the agitation speed and temperature at 200 rpm and 5...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36508/ http://psasir.upm.edu.my/id/eprint/36508/1/Application%20of%20different%20feeding%20strategies%20in%20fed%20batch%20culture%20for%20pullulanase%20production%20using%20sago%20starch.pdf |
| Summary: | The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2 L bioreactor. In batch culture, 0.0803 U/mL of pullulanase activity with specific activity of 0.0213 U/mg was produced by controlling the agitation speed and temperature at 200 rpm and 50 °C, respectively. Fed batch production was studied by feeding the culture with different sago starch concentrations in various feeding modes for enhanced pullulanase production. Exponential feeding mode at dilution rate of 0.01/h was the preeminent strategy for enhanced pullulanase production of 0.1710 U/mL with specific activity of 0.066 U/mg. It had shown an increment of pullulanase production and specific activity by 2.1 and 3.1-fold, respectively when compared to batch culture. Increment of pullulanase activity in exponential feeding mode improved hydrolyzation of sago starch into maltotriose and panose by 4.5 and 2.5-fold respectively compared to batch system. |
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