Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells
The antioxidant properties of germinated brown rice (GBR) are likely mediated by multiple bioactives. To test this hypothesis, HepG2 cells pretreated with GBR extracts, rich in acylated steryl glycoside (ASG), gamma amino butyric acid GABA), phenolics or oryzanol, were incubated with hydrogen peroxi...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36496/ http://psasir.upm.edu.my/id/eprint/36496/1/Antioxidative%20effects%20of%20germinated%20brown%20rice.pdf |
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| author | Md Zamri, Nur Diyana Imam, Mustapha Umar Abd Ghafar, Siti Aisyah Ismail, Maznah |
| author_facet | Md Zamri, Nur Diyana Imam, Mustapha Umar Abd Ghafar, Siti Aisyah Ismail, Maznah |
| author_sort | Md Zamri, Nur Diyana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The antioxidant properties of germinated brown rice (GBR) are likely mediated by multiple bioactives. To test this hypothesis, HepG2 cells pretreated with GBR extracts, rich in acylated steryl glycoside (ASG), gamma amino butyric acid GABA), phenolics or oryzanol, were incubated with hydrogen peroxide (H2O2) and their hydroxyl radical (OH•) scavenging capacities and thiobarbituric acid-reactive substances (TBARS) generation were evaluated. Results showed that GBR-extracts increased OH• scavenging activities in both cell-free medium and posttreatment culture media, suggesting that the extracts were both direct- and indirect-acting against OH•. The levels of TBARS in the culture medium after treatment were also reduced by all the extracts. In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Our findings show that GBR bioactive-rich extracts reduce oxidative stress through improvement in antioxidant capacity, partly mediated through transcriptional regulation of antioxidant and prosurvival genes. |
| first_indexed | 2025-11-15T09:33:05Z |
| format | Article |
| id | upm-36496 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:33:05Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-364962015-10-06T04:32:43Z http://psasir.upm.edu.my/id/eprint/36496/ Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells Md Zamri, Nur Diyana Imam, Mustapha Umar Abd Ghafar, Siti Aisyah Ismail, Maznah The antioxidant properties of germinated brown rice (GBR) are likely mediated by multiple bioactives. To test this hypothesis, HepG2 cells pretreated with GBR extracts, rich in acylated steryl glycoside (ASG), gamma amino butyric acid GABA), phenolics or oryzanol, were incubated with hydrogen peroxide (H2O2) and their hydroxyl radical (OH•) scavenging capacities and thiobarbituric acid-reactive substances (TBARS) generation were evaluated. Results showed that GBR-extracts increased OH• scavenging activities in both cell-free medium and posttreatment culture media, suggesting that the extracts were both direct- and indirect-acting against OH•. The levels of TBARS in the culture medium after treatment were also reduced by all the extracts. In addition, H2O2 produced transcriptional changes in p53, JNK, p38 MAPK, AKT, BAX, and CDK4 that were inclined towards apoptosis, while GBR-extracts showed some transcriptional changes (upregulation of BAX and p53) that suggested an inclination for apoptosis although other changes (upregulation of antioxidant genes, AKT, JNK, and p38 MAPK) suggested that GBR-extracts favored survival of the HepG2 cells. Our findings show that GBR bioactive-rich extracts reduce oxidative stress through improvement in antioxidant capacity, partly mediated through transcriptional regulation of antioxidant and prosurvival genes. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36496/1/Antioxidative%20effects%20of%20germinated%20brown%20rice.pdf Md Zamri, Nur Diyana and Imam, Mustapha Umar and Abd Ghafar, Siti Aisyah and Ismail, Maznah (2014) Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells. Evidence-Based Complementary and Alternative Medicine, 2014. art. no. 371907. pp. 1-11. ISSN 1741-427X; ESSN: 1741-4288 http://www.hindawi.com/journals/ecam/2014/371907/ 10.1155/2014/371907 |
| spellingShingle | Md Zamri, Nur Diyana Imam, Mustapha Umar Abd Ghafar, Siti Aisyah Ismail, Maznah Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells |
| title | Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells |
| title_full | Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells |
| title_fullStr | Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells |
| title_full_unstemmed | Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells |
| title_short | Antioxidative effects of germinated brown rice-derived extracts on H2O2-induced oxidative stress in HepG2 cells |
| title_sort | antioxidative effects of germinated brown rice-derived extracts on h2o2-induced oxidative stress in hepg2 cells |
| url | http://psasir.upm.edu.my/id/eprint/36496/ http://psasir.upm.edu.my/id/eprint/36496/ http://psasir.upm.edu.my/id/eprint/36496/ http://psasir.upm.edu.my/id/eprint/36496/1/Antioxidative%20effects%20of%20germinated%20brown%20rice.pdf |