Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops
Stemphylium lycopersici (Enjoji) W. Yamam was initially described from tomato and has been reported to infect different hosts worldwide. Sequence analyses of the internal transcribed spacer (ITS) regions 1 and 2, including 5.8S rDNA (ITS-5.8S rDNA) and glyceraldehyde-3-phosphate dehydrogenase (gpd)...
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| Format: | Article |
| Language: | English |
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Springer Netherlands
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36448/ http://psasir.upm.edu.my/id/eprint/36448/1/Analysis%20of%20genetic%20and%20virulence%20variability%20of%20Stemphylium%20lycopersici%20associated%20with%20leaf%20spot%20of%20vegetable%20crops.pdf |
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| author | Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Ashtiani, Farnaz Abed Wong, Mui Yun Rambe, Siti Khadijah Golkhandan, Elham |
| author_facet | Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Ashtiani, Farnaz Abed Wong, Mui Yun Rambe, Siti Khadijah Golkhandan, Elham |
| author_sort | Nasehi, Abbas |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Stemphylium lycopersici (Enjoji) W. Yamam was initially described from tomato and has been reported to infect different hosts worldwide. Sequence analyses of the internal transcribed spacer (ITS) regions 1 and 2, including 5.8S rDNA (ITS-5.8S rDNA) and glyceraldehyde-3-phosphate dehydrogenase (gpd) gene, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), as well as virulence studies were conducted to analyze 46 S. lycopersici isolates. Stemphylium lycopersici isolates used in this study were obtained from diseased tomato (Solanum lycopersicum L.), eggplant (Solanum melongena L.), pepper (Capsicum annuum L.) and lettuce (Lactuca sativa L.) from major vegetable growing regions of Malaysia, including the three states of Pahang, Johor and Selangor between 2011 and 2012. Phylogenetic analysis of a combined dataset of the ITS-5.8S rDNA and gpd regions indicated that all isolates were clustered in the sub-cluster that comprised S. lycopersici, and were distinguished from other Stemphylium species. Cluster analyses using the UPGMA method for both RAPD and ISSR markers grouped S. lycopersici isolates into three main clusters with similarity index values of 67 and 68 %. The genetic diversity data confirmed that isolates of S. lycopersici are in concordance to host plants, and not geographical origin of the isolates. All S. lycopersici isolates were pathogenic on their original host plants and showed leaf spot symptoms; however, virulence variability was observed among the isolates. In cross-inoculation assays, the representative isolates were able to cause leaf spot symptoms on eggplant, pepper, lettuce and tomato, but not on cabbage. |
| first_indexed | 2025-11-15T09:32:52Z |
| format | Article |
| id | upm-36448 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:32:52Z |
| publishDate | 2014 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-364482015-12-03T07:20:54Z http://psasir.upm.edu.my/id/eprint/36448/ Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Ashtiani, Farnaz Abed Wong, Mui Yun Rambe, Siti Khadijah Golkhandan, Elham Stemphylium lycopersici (Enjoji) W. Yamam was initially described from tomato and has been reported to infect different hosts worldwide. Sequence analyses of the internal transcribed spacer (ITS) regions 1 and 2, including 5.8S rDNA (ITS-5.8S rDNA) and glyceraldehyde-3-phosphate dehydrogenase (gpd) gene, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), as well as virulence studies were conducted to analyze 46 S. lycopersici isolates. Stemphylium lycopersici isolates used in this study were obtained from diseased tomato (Solanum lycopersicum L.), eggplant (Solanum melongena L.), pepper (Capsicum annuum L.) and lettuce (Lactuca sativa L.) from major vegetable growing regions of Malaysia, including the three states of Pahang, Johor and Selangor between 2011 and 2012. Phylogenetic analysis of a combined dataset of the ITS-5.8S rDNA and gpd regions indicated that all isolates were clustered in the sub-cluster that comprised S. lycopersici, and were distinguished from other Stemphylium species. Cluster analyses using the UPGMA method for both RAPD and ISSR markers grouped S. lycopersici isolates into three main clusters with similarity index values of 67 and 68 %. The genetic diversity data confirmed that isolates of S. lycopersici are in concordance to host plants, and not geographical origin of the isolates. All S. lycopersici isolates were pathogenic on their original host plants and showed leaf spot symptoms; however, virulence variability was observed among the isolates. In cross-inoculation assays, the representative isolates were able to cause leaf spot symptoms on eggplant, pepper, lettuce and tomato, but not on cabbage. Springer Netherlands 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36448/1/Analysis%20of%20genetic%20and%20virulence%20variability%20of%20Stemphylium%20lycopersici%20associated%20with%20leaf%20spot%20of%20vegetable%20crops.pdf Nasehi, Abbas and Kadir, Jugah and Esfahani, Mehdi Nasr and Ashtiani, Farnaz Abed and Wong, Mui Yun and Rambe, Siti Khadijah and Golkhandan, Elham (2014) Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops. European Journal of Plant Pathology, 140 (2). pp. 261-273. ISSN 0929-1873; ESSN: 1573-8469 10.1007/s10658-014-0460-3 |
| spellingShingle | Nasehi, Abbas Kadir, Jugah Esfahani, Mehdi Nasr Ashtiani, Farnaz Abed Wong, Mui Yun Rambe, Siti Khadijah Golkhandan, Elham Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops |
| title | Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops |
| title_full | Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops |
| title_fullStr | Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops |
| title_full_unstemmed | Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops |
| title_short | Analysis of genetic and virulence variability of Stemphylium lycopersici associated with leaf spot of vegetable crops |
| title_sort | analysis of genetic and virulence variability of stemphylium lycopersici associated with leaf spot of vegetable crops |
| url | http://psasir.upm.edu.my/id/eprint/36448/ http://psasir.upm.edu.my/id/eprint/36448/ http://psasir.upm.edu.my/id/eprint/36448/1/Analysis%20of%20genetic%20and%20virulence%20variability%20of%20Stemphylium%20lycopersici%20associated%20with%20leaf%20spot%20of%20vegetable%20crops.pdf |