Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide
The gram-negative bacterium Pasteurella multocida serotype B: 2 is an important veterinary and opportunistic human pathogen that causes deplorable welfare problems and colossal economic production losses in bovine and other animal species throughout the world. Pasteurella multocida type B: 2 and its...
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| Format: | Article |
| Language: | English |
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Science Publications
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36369/ http://psasir.upm.edu.my/id/eprint/36369/1/ajavsp.2015.1.8.pdf |
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| author | Ali, Omar Suwaidan Adamu, Lawan Abdullah, Faez Firdaus Jesse Ilyasu, Yusuf Abba, Yusuf Hamzah, Hazilawati Mohd Lila, Mohd Azmi Haron, Abd Wahid Saad, Mohd Zamri |
| author_facet | Ali, Omar Suwaidan Adamu, Lawan Abdullah, Faez Firdaus Jesse Ilyasu, Yusuf Abba, Yusuf Hamzah, Hazilawati Mohd Lila, Mohd Azmi Haron, Abd Wahid Saad, Mohd Zamri |
| author_sort | Ali, Omar Suwaidan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The gram-negative bacterium Pasteurella multocida serotype B: 2 is an important veterinary and opportunistic human pathogen that causes deplorable welfare problems and colossal economic production losses in bovine and other animal species throughout the world. Pasteurella multocida type B: 2 and its LPS are associated with Haemorrhagic Septicaemia (HS) in mice as animal model. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n = 10) and these were inoculated with 0.4 mL of PBS pH 7.4 orally. The treatment groups (B; n = 25 and C; n = 25) were inoculated with P. multocida type B: 2 and lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B101, B103, B105, B107 and B109 for Pasteurella multocida and C101, C103, C105, C107 and C109 for LPS respectively. The mice were observed for 120 h post-inoculation. The concentration of IL-1β was significantly higher (p<0.001) in the B107 of P. multocida type B: 2 and C109 of LPS compared to the control group and the other treatment groups. The concentration of IL-6 was significantly higher (p<0.001) in the B103 cfu of P. multocida type B: 2 and C109 cfu of LPS compared to the control group and the other treatment groups. Interleukin-1β and interleukin-6 evaluated in this study were predominantly associated with HS in varying doses of 101, 103, 105, 107 and 109 in the whole cell and its LPS respectively. Therefore, the target of this study was to investigate on pro-inflammatory response in mice infected with graded doses of Pasteurella multocida serotype B: 2 and its LPS via the oral route of inoculation. |
| first_indexed | 2025-11-15T09:32:30Z |
| format | Article |
| id | upm-36369 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:32:30Z |
| publishDate | 2015 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-363692017-11-22T03:34:14Z http://psasir.upm.edu.my/id/eprint/36369/ Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide Ali, Omar Suwaidan Adamu, Lawan Abdullah, Faez Firdaus Jesse Ilyasu, Yusuf Abba, Yusuf Hamzah, Hazilawati Mohd Lila, Mohd Azmi Haron, Abd Wahid Saad, Mohd Zamri The gram-negative bacterium Pasteurella multocida serotype B: 2 is an important veterinary and opportunistic human pathogen that causes deplorable welfare problems and colossal economic production losses in bovine and other animal species throughout the world. Pasteurella multocida type B: 2 and its LPS are associated with Haemorrhagic Septicaemia (HS) in mice as animal model. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n = 10) and these were inoculated with 0.4 mL of PBS pH 7.4 orally. The treatment groups (B; n = 25 and C; n = 25) were inoculated with P. multocida type B: 2 and lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B101, B103, B105, B107 and B109 for Pasteurella multocida and C101, C103, C105, C107 and C109 for LPS respectively. The mice were observed for 120 h post-inoculation. The concentration of IL-1β was significantly higher (p<0.001) in the B107 of P. multocida type B: 2 and C109 of LPS compared to the control group and the other treatment groups. The concentration of IL-6 was significantly higher (p<0.001) in the B103 cfu of P. multocida type B: 2 and C109 cfu of LPS compared to the control group and the other treatment groups. Interleukin-1β and interleukin-6 evaluated in this study were predominantly associated with HS in varying doses of 101, 103, 105, 107 and 109 in the whole cell and its LPS respectively. Therefore, the target of this study was to investigate on pro-inflammatory response in mice infected with graded doses of Pasteurella multocida serotype B: 2 and its LPS via the oral route of inoculation. Science Publications 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36369/1/ajavsp.2015.1.8.pdf Ali, Omar Suwaidan and Adamu, Lawan and Abdullah, Faez Firdaus Jesse and Ilyasu, Yusuf and Abba, Yusuf and Hamzah, Hazilawati and Mohd Lila, Mohd Azmi and Haron, Abd Wahid and Saad, Mohd Zamri (2015) Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide. American Journal of Animal and Veterinary Sciences, 10 (1). pp. 1-8. ISSN 1557-4555; ESSN: 1557-4563 http://thescipub.com/abstract/10.3844/ajavsp.2015.1.8 10.3844/ajavsp.2015.1.8 |
| spellingShingle | Ali, Omar Suwaidan Adamu, Lawan Abdullah, Faez Firdaus Jesse Ilyasu, Yusuf Abba, Yusuf Hamzah, Hazilawati Mohd Lila, Mohd Azmi Haron, Abd Wahid Saad, Mohd Zamri Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide |
| title | Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide |
| title_full | Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide |
| title_fullStr | Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide |
| title_full_unstemmed | Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide |
| title_short | Alterations in Interleukin-1β and Interleukin-6 in mice inoculated through the oral routes using graded doses of p. Multocida type b: 2 and its lipopolysaccharide |
| title_sort | alterations in interleukin-1β and interleukin-6 in mice inoculated through the oral routes using graded doses of p. multocida type b: 2 and its lipopolysaccharide |
| url | http://psasir.upm.edu.my/id/eprint/36369/ http://psasir.upm.edu.my/id/eprint/36369/ http://psasir.upm.edu.my/id/eprint/36369/ http://psasir.upm.edu.my/id/eprint/36369/1/ajavsp.2015.1.8.pdf |