A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method

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A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method

A single-step purification method based on an anion exchange chromatography was developed to purify truncated Nipah virus glycoprotein (tNiVG) expressed in Spodoptera frugiperda 9 (Sf9) insect cells. The preferred buffer conditions to bind and elute tNiVG protein were 50 mM sodium carbonate with pH...

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Bibliographic Details
Main Authors:	Raksha, Sunhare, Tan, Wen Siang, Hamid, Muhajir, Ramanan, Ramakrishnan Nagasundara, Tey, Beng Ti
Format:	Article
Language:	English
Published:	Taylor & Francis 2014
Online Access:	http://psasir.upm.edu.my/id/eprint/36282/ http://psasir.upm.edu.my/id/eprint/36282/1/A%20single.pdf

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