A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method
A single-step purification method based on an anion exchange chromatography was developed to purify truncated Nipah virus glycoprotein (tNiVG) expressed in Spodoptera frugiperda 9 (Sf9) insect cells. The preferred buffer conditions to bind and elute tNiVG protein were 50 mM sodium carbonate with pH...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36282/ http://psasir.upm.edu.my/id/eprint/36282/1/A%20single.pdf |
| _version_ | 1848848288794542080 |
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| author | Raksha, Sunhare Tan, Wen Siang Hamid, Muhajir Ramanan, Ramakrishnan Nagasundara Tey, Beng Ti |
| author_facet | Raksha, Sunhare Tan, Wen Siang Hamid, Muhajir Ramanan, Ramakrishnan Nagasundara Tey, Beng Ti |
| author_sort | Raksha, Sunhare |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A single-step purification method based on an anion exchange chromatography was developed to purify truncated Nipah virus glycoprotein (tNiVG) expressed in Spodoptera frugiperda 9 (Sf9) insect cells. The preferred buffer conditions to bind and elute tNiVG protein were 50 mM sodium carbonate with pH 9 and 50 mM sodium citrate with pH 5, respectively. The use of elution buffer without sodium chloride separated the tNiVG protein from the tightly bound major host proteins and subsequently avoided the desalting step as one of the further downstream processes. About 90% purity and 89% recovery of tNiVG protein were achieved with the developed purification method. |
| first_indexed | 2025-11-15T09:32:07Z |
| format | Article |
| id | upm-36282 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:32:07Z |
| publishDate | 2014 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-362822015-10-29T08:02:23Z http://psasir.upm.edu.my/id/eprint/36282/ A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method Raksha, Sunhare Tan, Wen Siang Hamid, Muhajir Ramanan, Ramakrishnan Nagasundara Tey, Beng Ti A single-step purification method based on an anion exchange chromatography was developed to purify truncated Nipah virus glycoprotein (tNiVG) expressed in Spodoptera frugiperda 9 (Sf9) insect cells. The preferred buffer conditions to bind and elute tNiVG protein were 50 mM sodium carbonate with pH 9 and 50 mM sodium citrate with pH 5, respectively. The use of elution buffer without sodium chloride separated the tNiVG protein from the tightly bound major host proteins and subsequently avoided the desalting step as one of the further downstream processes. About 90% purity and 89% recovery of tNiVG protein were achieved with the developed purification method. Taylor & Francis 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36282/1/A%20single.pdf Raksha, Sunhare and Tan, Wen Siang and Hamid, Muhajir and Ramanan, Ramakrishnan Nagasundara and Tey, Beng Ti (2014) A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method. Separation Science and Technology, 49 (2). pp. 249-257. ISSN 0149-6395; ESSN:1520-5754 10.1080/01496395.2013.838265 |
| spellingShingle | Raksha, Sunhare Tan, Wen Siang Hamid, Muhajir Ramanan, Ramakrishnan Nagasundara Tey, Beng Ti A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method |
| title | A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method |
| title_full | A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method |
| title_fullStr | A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method |
| title_full_unstemmed | A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method |
| title_short | A single-step purification of the glycoprotein of Nipah virus produced in insect cells using an anion exchange chromatography method |
| title_sort | single-step purification of the glycoprotein of nipah virus produced in insect cells using an anion exchange chromatography method |
| url | http://psasir.upm.edu.my/id/eprint/36282/ http://psasir.upm.edu.my/id/eprint/36282/ http://psasir.upm.edu.my/id/eprint/36282/1/A%20single.pdf |