Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles
Influenza A virus poses a major threat to human health, causing outbreaks from time to time. Currently available vaccines employ inactivated viruses of different strains to provide protection against influenza virus infection. However, high mutation rates of influenza virus hemagglutinin (H) and neu...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36228/ http://psasir.upm.edu.my/id/eprint/36228/1/Potential%20recombinant%20vaccine%20against%20influenza%20A%20virus%20based%20on%20M2e%20displayed%20on%20nodaviral%20capsid%20nanoparticles.pdf |
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| author | Yong, Chean Yeah Yeap, Swee Keong Ho, Kok Lian Omar, Abdul Rahman Tan, Wen Siang |
| author_facet | Yong, Chean Yeah Yeap, Swee Keong Ho, Kok Lian Omar, Abdul Rahman Tan, Wen Siang |
| author_sort | Yong, Chean Yeah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Influenza A virus poses a major threat to human health, causing outbreaks from time to time. Currently available vaccines employ inactivated viruses of different strains to provide protection against influenza virus infection. However, high mutation rates of influenza virus hemagglutinin (H) and neuraminidase (N) glycoproteins give rise to vaccine escape mutants. Thus, an effective vaccine providing protection against all strains of influenza virus would be a valuable asset. The ectodomain of matrix 2 protein (M2e) was found to be highly conserved despite mutations of the H and N glycoproteins. Hence, one to five copies of M2e were fused to the carboxyl-terminal end of the recombinant nodavirus capsid protein derived from Macrobrachium rosenbergii. The chimeric proteins harboring up to five copies of M2e formed nanosized virus-like particles approximately 30 nm in diameter, which could be purified easily by immobilized metal affinity chromatography. BALB/c mice immunized subcutaneously with these chimeric proteins developed antibodies specifically against M2e, and the titer was proportional to the copy numbers of M2e displayed on the nodavirus capsid nanoparticles. The fusion proteins also induced a type 1 T helper immune response. Collectively, M2e displayed on the nodavirus capsid nanoparticles could provide an alternative solution to a possible influenza pandemic in the future. |
| first_indexed | 2025-11-15T09:31:53Z |
| format | Article |
| id | upm-36228 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:31:53Z |
| publishDate | 2015 |
| publisher | Dove Medical Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-362282015-10-22T03:15:58Z http://psasir.upm.edu.my/id/eprint/36228/ Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles Yong, Chean Yeah Yeap, Swee Keong Ho, Kok Lian Omar, Abdul Rahman Tan, Wen Siang Influenza A virus poses a major threat to human health, causing outbreaks from time to time. Currently available vaccines employ inactivated viruses of different strains to provide protection against influenza virus infection. However, high mutation rates of influenza virus hemagglutinin (H) and neuraminidase (N) glycoproteins give rise to vaccine escape mutants. Thus, an effective vaccine providing protection against all strains of influenza virus would be a valuable asset. The ectodomain of matrix 2 protein (M2e) was found to be highly conserved despite mutations of the H and N glycoproteins. Hence, one to five copies of M2e were fused to the carboxyl-terminal end of the recombinant nodavirus capsid protein derived from Macrobrachium rosenbergii. The chimeric proteins harboring up to five copies of M2e formed nanosized virus-like particles approximately 30 nm in diameter, which could be purified easily by immobilized metal affinity chromatography. BALB/c mice immunized subcutaneously with these chimeric proteins developed antibodies specifically against M2e, and the titer was proportional to the copy numbers of M2e displayed on the nodavirus capsid nanoparticles. The fusion proteins also induced a type 1 T helper immune response. Collectively, M2e displayed on the nodavirus capsid nanoparticles could provide an alternative solution to a possible influenza pandemic in the future. Dove Medical Press 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36228/1/Potential%20recombinant%20vaccine%20against%20influenza%20A%20virus%20based%20on%20M2e%20displayed%20on%20nodaviral%20capsid%20nanoparticles.pdf Yong, Chean Yeah and Yeap, Swee Keong and Ho, Kok Lian and Omar, Abdul Rahman and Tan, Wen Siang (2015) Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles. International Journal of Nanomedicine, 10. pp. 2751-2763. ISSN 1178-2013 http://www.dovepress.com/potential-recombinant-vaccine-against-influenza-a-virus-based-on-m2e-d-peer-reviewed-article-IJN 10.2147/IJN.S77405 |
| spellingShingle | Yong, Chean Yeah Yeap, Swee Keong Ho, Kok Lian Omar, Abdul Rahman Tan, Wen Siang Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles |
| title | Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles |
| title_full | Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles |
| title_fullStr | Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles |
| title_full_unstemmed | Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles |
| title_short | Potential recombinant vaccine against influenza A virus based on M2e displayed on nodaviral capsid nanoparticles |
| title_sort | potential recombinant vaccine against influenza a virus based on m2e displayed on nodaviral capsid nanoparticles |
| url | http://psasir.upm.edu.my/id/eprint/36228/ http://psasir.upm.edu.my/id/eprint/36228/ http://psasir.upm.edu.my/id/eprint/36228/ http://psasir.upm.edu.my/id/eprint/36228/1/Potential%20recombinant%20vaccine%20against%20influenza%20A%20virus%20based%20on%20M2e%20displayed%20on%20nodaviral%20capsid%20nanoparticles.pdf |