A higher sensitivity and efficiency of common primer multiplex PCR assay in identification of meat origin using NADH dehydrogenase subunit 4 gene
A Common Primer Multiplex PCR (CP-M-PCR) was developed to detect meat origin of four groups of animal (pig, ruminant, avian and rabbit). This method demonstrated higher sensitivity and efficiency than the conventional multiplex PCR. In this approach, a common forward primer was designed in the 5′ en...
| Main Authors: | Hanapi, Ummi Kalthum, Mohd Desa, Mohd Nasir, Ismail, Amin, Mustafa, Shuhaimi |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/36170/ http://psasir.upm.edu.my/id/eprint/36170/1/A%20higher%20sensitivity%20and%20efficiency%20of%20common%20primer%20multiplex%20PCR%20assay%20in%20identification%20of%20meat%20origin%20using%20NADH%20dehydrogenase%20subunit%204%20gene.pdf |
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