Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line
This investigation evaluated the antileukemia properties of a zerumbone (ZER)-loaded nanostructured lipid carrier (NLC) prepared by hot high-pressure homogenization techniques in an acute human lymphoblastic leukemia (Jurkat) cell line in vitro. The apoptogenic effect of the ZER-NLC on Jurkat cells...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/36045/ http://psasir.upm.edu.my/id/eprint/36045/1/36045.pdf |
| _version_ | 1848848223745081344 |
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| author | Rahman, Heshu Sulaiman Abdullah, Rasedee Abdul, Ahmad Bustamam Allaudin, Zeenathul Nazariah Othman, Hemn Hassan Yeap, Swee Keong How, Chee Wun Wan Abd Ghani, Wan Nor Hafiza |
| author_facet | Rahman, Heshu Sulaiman Abdullah, Rasedee Abdul, Ahmad Bustamam Allaudin, Zeenathul Nazariah Othman, Hemn Hassan Yeap, Swee Keong How, Chee Wun Wan Abd Ghani, Wan Nor Hafiza |
| author_sort | Rahman, Heshu Sulaiman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This investigation evaluated the antileukemia properties of a zerumbone (ZER)-loaded nanostructured lipid carrier (NLC) prepared by hot high-pressure homogenization techniques in an acute human lymphoblastic leukemia (Jurkat) cell line in vitro. The apoptogenic effect of the ZER-NLC on Jurkat cells was determined by fluorescent and electron microscopy, Annexin V-fluorescein isothiocyanate, Tdt-mediated dUTP nick-end labeling assay, cell cycle analysis, and caspase activity. An MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay showed that ZER-NLC did not have adverse effects on normal human peripheral blood mononuclear cells. ZER-NLC arrested the Jurkat cells at G2/M phase with inactivation of cyclin B1 protein. The study also showed that the antiproliferative effect of ZER-NLC on Jurkat cells is through the intrinsic apoptotic pathway via activation of caspase-3 and caspase-9, release of cytochrome c from the mitochondria into the cytosol, and subsequent cleavage of poly (adenosine diphosphate-ribose) polymerase (PARP). These findings show that the ZER-NLC is a potentially useful treatment for acute lymphoblastic leukemia in humans. |
| first_indexed | 2025-11-15T09:31:05Z |
| format | Article |
| id | upm-36045 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:31:05Z |
| publishDate | 2014 |
| publisher | Dove Medical Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-360452019-10-30T07:13:42Z http://psasir.upm.edu.my/id/eprint/36045/ Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line Rahman, Heshu Sulaiman Abdullah, Rasedee Abdul, Ahmad Bustamam Allaudin, Zeenathul Nazariah Othman, Hemn Hassan Yeap, Swee Keong How, Chee Wun Wan Abd Ghani, Wan Nor Hafiza This investigation evaluated the antileukemia properties of a zerumbone (ZER)-loaded nanostructured lipid carrier (NLC) prepared by hot high-pressure homogenization techniques in an acute human lymphoblastic leukemia (Jurkat) cell line in vitro. The apoptogenic effect of the ZER-NLC on Jurkat cells was determined by fluorescent and electron microscopy, Annexin V-fluorescein isothiocyanate, Tdt-mediated dUTP nick-end labeling assay, cell cycle analysis, and caspase activity. An MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay showed that ZER-NLC did not have adverse effects on normal human peripheral blood mononuclear cells. ZER-NLC arrested the Jurkat cells at G2/M phase with inactivation of cyclin B1 protein. The study also showed that the antiproliferative effect of ZER-NLC on Jurkat cells is through the intrinsic apoptotic pathway via activation of caspase-3 and caspase-9, release of cytochrome c from the mitochondria into the cytosol, and subsequent cleavage of poly (adenosine diphosphate-ribose) polymerase (PARP). These findings show that the ZER-NLC is a potentially useful treatment for acute lymphoblastic leukemia in humans. Dove Medical Press 2014 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/36045/1/36045.pdf Rahman, Heshu Sulaiman and Abdullah, Rasedee and Abdul, Ahmad Bustamam and Allaudin, Zeenathul Nazariah and Othman, Hemn Hassan and Yeap, Swee Keong and How, Chee Wun and Wan Abd Ghani, Wan Nor Hafiza (2014) Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line. International Journal of Nanomedicine, 9 (1). pp. 527-538. ISSN 1176-9114; ESSN: 1178-2013 https://www.dovepress.com/zerumbone-loaded-nanostructured-lipid-carrier-induces-g2m-cell-cycle-a-peer-reviewed-article-IJN 10.2147/IJN.S54346 |
| spellingShingle | Rahman, Heshu Sulaiman Abdullah, Rasedee Abdul, Ahmad Bustamam Allaudin, Zeenathul Nazariah Othman, Hemn Hassan Yeap, Swee Keong How, Chee Wun Wan Abd Ghani, Wan Nor Hafiza Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line |
| title | Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line |
| title_full | Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line |
| title_fullStr | Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line |
| title_full_unstemmed | Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line |
| title_short | Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line |
| title_sort | zerumbone-loaded nanostructured lipid carrier induces g2/m cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line |
| url | http://psasir.upm.edu.my/id/eprint/36045/ http://psasir.upm.edu.my/id/eprint/36045/ http://psasir.upm.edu.my/id/eprint/36045/ http://psasir.upm.edu.my/id/eprint/36045/1/36045.pdf |