Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus

Eight previously designed polymorphic microsatellite loci, namely, Mnc434a, Mnc65b, Mnc441, MnBp5-1-02b, MnBp5-1-30b, MnBp5-2-02b, MnBp8-4-43a, and MnBp8-4-43b, were used to examine their modes ofsegregation in Mystus nemurus family study. Randomly selected broodstocks from Terengganu population w...

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Main Authors: Hoh, Boon Peng, Siraj, Siti Shapor, Tan, Soon Guan, Yusoff, Khatijah
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2005
Online Access:http://psasir.upm.edu.my/id/eprint/3604/
http://psasir.upm.edu.my/id/eprint/3604/1/Mendelian_Inheritance_of_Microsatellite_Markers_in.pdf
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author Hoh, Boon Peng
Siraj, Siti Shapor
Tan, Soon Guan
Yusoff, Khatijah
author_facet Hoh, Boon Peng
Siraj, Siti Shapor
Tan, Soon Guan
Yusoff, Khatijah
author_sort Hoh, Boon Peng
building UPM Institutional Repository
collection Online Access
description Eight previously designed polymorphic microsatellite loci, namely, Mnc434a, Mnc65b, Mnc441, MnBp5-1-02b, MnBp5-1-30b, MnBp5-2-02b, MnBp8-4-43a, and MnBp8-4-43b, were used to examine their modes ofsegregation in Mystus nemurus family study. Randomly selected broodstocks from Terengganu population were used to generate F 1 population. DNA was isolated from tissue samples of the parents, whilst the whole one-month-old F , 1 progeny was used to isolate the DNA. Chi-square analysis was performed to test the significance of the results obtained. It was shown that all the eight microsatellite loci examined segregated close to Mendelian inheritance ratios, which would be useful in generating a genetic linkage map for this species.
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spelling upm-36042015-09-15T01:28:25Z http://psasir.upm.edu.my/id/eprint/3604/ Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus Hoh, Boon Peng Siraj, Siti Shapor Tan, Soon Guan Yusoff, Khatijah Eight previously designed polymorphic microsatellite loci, namely, Mnc434a, Mnc65b, Mnc441, MnBp5-1-02b, MnBp5-1-30b, MnBp5-2-02b, MnBp8-4-43a, and MnBp8-4-43b, were used to examine their modes ofsegregation in Mystus nemurus family study. Randomly selected broodstocks from Terengganu population were used to generate F 1 population. DNA was isolated from tissue samples of the parents, whilst the whole one-month-old F , 1 progeny was used to isolate the DNA. Chi-square analysis was performed to test the significance of the results obtained. It was shown that all the eight microsatellite loci examined segregated close to Mendelian inheritance ratios, which would be useful in generating a genetic linkage map for this species. Universiti Putra Malaysia Press 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3604/1/Mendelian_Inheritance_of_Microsatellite_Markers_in.pdf Hoh, Boon Peng and Siraj, Siti Shapor and Tan, Soon Guan and Yusoff, Khatijah (2005) Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus. Pertanika Journal of Tropical Agricultural Science, 28 (1). pp. 67-71. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(67-71).pdf
spellingShingle Hoh, Boon Peng
Siraj, Siti Shapor
Tan, Soon Guan
Yusoff, Khatijah
Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus
title Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus
title_full Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus
title_fullStr Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus
title_full_unstemmed Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus
title_short Mendelian inheritance of microsatellite markers in Southeast Asian River Catfish, Mystus nemurus
title_sort mendelian inheritance of microsatellite markers in southeast asian river catfish, mystus nemurus
url http://psasir.upm.edu.my/id/eprint/3604/
http://psasir.upm.edu.my/id/eprint/3604/
http://psasir.upm.edu.my/id/eprint/3604/1/Mendelian_Inheritance_of_Microsatellite_Markers_in.pdf