Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis
A total of 21 DNA microsatellites were isolated from Perna viridis lJy using a 5' anchored polymerase chain reaction technique. Primers were designed for seven microsatellite loci and the peR amplifications of these seven microsatellite loci showed that five were polymorphic with the number of...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3602/ http://psasir.upm.edu.my/id/eprint/3602/1/Isolation_of_DNA_Microsatellite_Markers_in_the.pdf |
| _version_ | 1848839574988521472 |
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| author | Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong |
| author_facet | Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong |
| author_sort | Ong, Lily Chin Chin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A total of 21 DNA microsatellites were isolated from Perna viridis lJy using a 5' anchored polymerase chain
reaction technique. Primers were designed for seven microsatellite loci and the peR amplifications of these seven microsatellite loci showed that five were polymorphic with the number ofalleles per locus rangingfrom 2 to 4. These are the first set of microsatellite markers developed for this species. These markers are useful as tools for more
detailed studies of the genetic backgrounds of the green-lipped musse~ P. viridis, in our country as this species
is being cultured as a cheap source of protein for human consumption and has been identified as a potential
biomonitoring agent for heavy metal pollution in the west coast of Peninsular Malaysia. |
| first_indexed | 2025-11-15T07:13:37Z |
| format | Article |
| id | upm-3602 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:13:37Z |
| publishDate | 2005 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-36022015-09-15T01:47:31Z http://psasir.upm.edu.my/id/eprint/3602/ Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong A total of 21 DNA microsatellites were isolated from Perna viridis lJy using a 5' anchored polymerase chain reaction technique. Primers were designed for seven microsatellite loci and the peR amplifications of these seven microsatellite loci showed that five were polymorphic with the number ofalleles per locus rangingfrom 2 to 4. These are the first set of microsatellite markers developed for this species. These markers are useful as tools for more detailed studies of the genetic backgrounds of the green-lipped musse~ P. viridis, in our country as this species is being cultured as a cheap source of protein for human consumption and has been identified as a potential biomonitoring agent for heavy metal pollution in the west coast of Peninsular Malaysia. Universiti Putra Malaysia Press 2005 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3602/1/Isolation_of_DNA_Microsatellite_Markers_in_the.pdf Ong, Lily Chin Chin and Tan, Soon Guan and Yusoff, Khatijah and Yap, Chee Kong (2005) Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis. Pertanika Journal of Tropical Agricultural Science, 28 (1). pp. 79-85. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2028%20(1)%20Apr.%202005/JTAS%20Vol.28%20(1)%202005%20(79-85).pdf |
| spellingShingle | Ong, Lily Chin Chin Tan, Soon Guan Yusoff, Khatijah Yap, Chee Kong Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis |
| title | Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis |
| title_full | Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis |
| title_fullStr | Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis |
| title_full_unstemmed | Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis |
| title_short | Isolation of DNA Microsatellite Markers in the Green-lipped Mussel, Perna viridis |
| title_sort | isolation of dna microsatellite markers in the green-lipped mussel, perna viridis |
| url | http://psasir.upm.edu.my/id/eprint/3602/ http://psasir.upm.edu.my/id/eprint/3602/ http://psasir.upm.edu.my/id/eprint/3602/1/Isolation_of_DNA_Microsatellite_Markers_in_the.pdf |