Variables affecting the recombinant phytase production from Escherichia coli DH5a
Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombina...
| Main Authors: | , , , , , |
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| Format: | Article |
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Oriental Scientific Publishing
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35969/ |
| _version_ | 1848848202684432384 |
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| author | Mohd Ariff, Rafidah Abd. Manap, Mohd. Yazid Ideris, Aini Kassim, Azhar Farouk, Abd-El Aziem Meor Hussin, Anis Shobirin |
| author_facet | Mohd Ariff, Rafidah Abd. Manap, Mohd. Yazid Ideris, Aini Kassim, Azhar Farouk, Abd-El Aziem Meor Hussin, Anis Shobirin |
| author_sort | Mohd Ariff, Rafidah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombinant phytase production from Escherichia coli DH5±. Through the application of the Fractional Factorial Design, the variables of seed age, inoculum level, time of induction, L-arabinose concentration and post-induction time were highly significant for the recombinant phytase production as their p-values were less than 0.05.Thus, minimal changes of this factor mat effect recombinant phytase production from E. coli DH5±. |
| first_indexed | 2025-11-15T09:30:45Z |
| format | Article |
| id | upm-35969 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:30:45Z |
| publishDate | 2015 |
| publisher | Oriental Scientific Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-359692016-02-12T02:42:45Z http://psasir.upm.edu.my/id/eprint/35969/ Variables affecting the recombinant phytase production from Escherichia coli DH5a Mohd Ariff, Rafidah Abd. Manap, Mohd. Yazid Ideris, Aini Kassim, Azhar Farouk, Abd-El Aziem Meor Hussin, Anis Shobirin Screening for the important variables is part of the optimization study of enzyme production. It is important indetermining the influential variables that give a significant effect on the enzyme production. Thus, the objective of this study was to determine variables that are effecting the recombinant phytase production from Escherichia coli DH5±. Through the application of the Fractional Factorial Design, the variables of seed age, inoculum level, time of induction, L-arabinose concentration and post-induction time were highly significant for the recombinant phytase production as their p-values were less than 0.05.Thus, minimal changes of this factor mat effect recombinant phytase production from E. coli DH5±. Oriental Scientific Publishing 2015-05 Article PeerReviewed Mohd Ariff, Rafidah and Abd. Manap, Mohd. Yazid and Ideris, Aini and Kassim, Azhar and Farouk, Abd-El Aziem and Meor Hussin, Anis Shobirin (2015) Variables affecting the recombinant phytase production from Escherichia coli DH5a. Journal of Pure and Applied Microbiology, 9 (spec. May). pp. 257-263. ISSN 0973-7510 |
| spellingShingle | Mohd Ariff, Rafidah Abd. Manap, Mohd. Yazid Ideris, Aini Kassim, Azhar Farouk, Abd-El Aziem Meor Hussin, Anis Shobirin Variables affecting the recombinant phytase production from Escherichia coli DH5a |
| title | Variables affecting the recombinant phytase production from Escherichia coli DH5a |
| title_full | Variables affecting the recombinant phytase production from Escherichia coli DH5a |
| title_fullStr | Variables affecting the recombinant phytase production from Escherichia coli DH5a |
| title_full_unstemmed | Variables affecting the recombinant phytase production from Escherichia coli DH5a |
| title_short | Variables affecting the recombinant phytase production from Escherichia coli DH5a |
| title_sort | variables affecting the recombinant phytase production from escherichia coli dh5a |
| url | http://psasir.upm.edu.my/id/eprint/35969/ |