Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil
For the first time 5-hydroxymethyl-2-furaldehyde (HMF) was separated from crude palm oil (CPO), and its authenticity was determined using an RP-HPLC method. Separation was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on a Purospher Star RP-18e column...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Published: |
Elsevier
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/35957/ |
| _version_ | 1848848199135002624 |
|---|---|
| author | Ariffin, Abdul Azis Mohd Ghazali, Hasanah Kavousi, Parviz |
| author_facet | Ariffin, Abdul Azis Mohd Ghazali, Hasanah Kavousi, Parviz |
| author_sort | Ariffin, Abdul Azis |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | For the first time 5-hydroxymethyl-2-furaldehyde (HMF) was separated from crude palm oil (CPO), and its authenticity was determined using an RP-HPLC method. Separation was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on a Purospher Star RP-18e column (250 mm × 4.6 mm, 5.0 μm). The flow rate was adjusted to 1 ml/min and detection was performed at 284 nm. The method was validated, and results obtained exhibit a good recovery (95.58% to 98.39%). Assessment of precision showed that the relative standard deviations (RSD%) of retention times and peak areas of spiked samples were less than 0.59% and 2.66%, respectively. Further, the limit of detection (LOD) and LOQ were 0.02, 0.05 mg/kg, respectively, and the response was linear across the applied ranges. The crude palm oil samples analysed exhibited HMF content less than 2.27 mg/kg. |
| first_indexed | 2025-11-15T09:30:42Z |
| format | Article |
| id | upm-35957 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:30:42Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-359572016-02-12T02:37:49Z http://psasir.upm.edu.my/id/eprint/35957/ Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil Ariffin, Abdul Azis Mohd Ghazali, Hasanah Kavousi, Parviz For the first time 5-hydroxymethyl-2-furaldehyde (HMF) was separated from crude palm oil (CPO), and its authenticity was determined using an RP-HPLC method. Separation was accomplished with isocratic elution of a mobile phase comprising water and methanol (92:8 v/v) on a Purospher Star RP-18e column (250 mm × 4.6 mm, 5.0 μm). The flow rate was adjusted to 1 ml/min and detection was performed at 284 nm. The method was validated, and results obtained exhibit a good recovery (95.58% to 98.39%). Assessment of precision showed that the relative standard deviations (RSD%) of retention times and peak areas of spiked samples were less than 0.59% and 2.66%, respectively. Further, the limit of detection (LOD) and LOQ were 0.02, 0.05 mg/kg, respectively, and the response was linear across the applied ranges. The crude palm oil samples analysed exhibited HMF content less than 2.27 mg/kg. Elsevier 2014-07-01 Article PeerReviewed Ariffin, Abdul Azis and Mohd Ghazali, Hasanah and Kavousi, Parviz (2014) Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil. Food Chemistry, 154. pp. 102-107. ISSN 0308-8146; ESSN:1873-7072 http://www.sciencedirect.com/science/article/pii/S0308814613019626 10.1016/j.foodchem.2013.12.082 |
| spellingShingle | Ariffin, Abdul Azis Mohd Ghazali, Hasanah Kavousi, Parviz Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil |
| title | Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil |
| title_full | Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil |
| title_fullStr | Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil |
| title_full_unstemmed | Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil |
| title_short | Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil |
| title_sort | validation of a hplc method for determination of hydroxymethylfurfural in crude palm oil |
| url | http://psasir.upm.edu.my/id/eprint/35957/ http://psasir.upm.edu.my/id/eprint/35957/ http://psasir.upm.edu.my/id/eprint/35957/ |