Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.)

Molecular markers in oil palm characterization and breeding began two decades ago. Microsatellite markers are a system that is commonly used in oil palm research since its development. Monomorphic SSR markers have been eliminated from all evolutionary and population ge...

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Main Authors: Hama-Ali, Emad Omer, Tan, Soon Guan
Format: Article
Published: Shastri Education Trust 2014
Online Access:http://psasir.upm.edu.my/id/eprint/35948/
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author Hama-Ali, Emad Omer
Tan, Soon Guan
author_facet Hama-Ali, Emad Omer
Tan, Soon Guan
author_sort Hama-Ali, Emad Omer
building UPM Institutional Repository
collection Online Access
description Molecular markers in oil palm characterization and breeding began two decades ago. Microsatellite markers are a system that is commonly used in oil palm research since its development. Monomorphic SSR markers have been eliminated from all evolutionary and population genetics studies by researchers because of their lack of genetic variability. The goals of this study were to review polymorphic DNA microsatellite marker system also known as simple sequence repeats(SSR) in oil palm research since its development and to employa monomorphic SSR marker for detection of illegitimacy in oil palm breeding programs. Ten monomorphic SSR markers and two half-sib families were used in this study. Illegitimate offspring IDs 97 and 180 were found by four monomorphic locimEgCIR0425, mEgCIR3477, mEgCIR3769, and mEgCIR3902 in Family-1and Family-2. In addition, five loci (mEgCIR3574, mEgCIR3607, mEgCIR3672, mEgCIR3785 and mEgCIR3807) detect one illegitimate offspring ID 180.This study showed that monomorphic SSR markers are suitable for the detection of illegitimate offsprings in oil palm breeding programs.
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spelling upm-359482016-02-18T00:46:15Z http://psasir.upm.edu.my/id/eprint/35948/ Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.) Hama-Ali, Emad Omer Tan, Soon Guan Molecular markers in oil palm characterization and breeding began two decades ago. Microsatellite markers are a system that is commonly used in oil palm research since its development. Monomorphic SSR markers have been eliminated from all evolutionary and population genetics studies by researchers because of their lack of genetic variability. The goals of this study were to review polymorphic DNA microsatellite marker system also known as simple sequence repeats(SSR) in oil palm research since its development and to employa monomorphic SSR marker for detection of illegitimacy in oil palm breeding programs. Ten monomorphic SSR markers and two half-sib families were used in this study. Illegitimate offspring IDs 97 and 180 were found by four monomorphic locimEgCIR0425, mEgCIR3477, mEgCIR3769, and mEgCIR3902 in Family-1and Family-2. In addition, five loci (mEgCIR3574, mEgCIR3607, mEgCIR3672, mEgCIR3785 and mEgCIR3807) detect one illegitimate offspring ID 180.This study showed that monomorphic SSR markers are suitable for the detection of illegitimate offsprings in oil palm breeding programs. Shastri Education Trust 2014 Article PeerReviewed Hama-Ali, Emad Omer and Tan, Soon Guan (2014) Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.). Research and Reviews: Journal of Botanical Sciences, 3 (4). pp. 1-6. ISSN 2347-2308; ESSN: 2320-0189 http://www.rroij.com/ArchiveJBS/botanical-sciences-archive.php?month=December&year=2014
spellingShingle Hama-Ali, Emad Omer
Tan, Soon Guan
Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.)
title Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.)
title_full Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.)
title_fullStr Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.)
title_full_unstemmed Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.)
title_short Using monomorphic microsatellite markers in oil palm (Elaeisguineensis Jacq.)
title_sort using monomorphic microsatellite markers in oil palm (elaeisguineensis jacq.)
url http://psasir.upm.edu.my/id/eprint/35948/
http://psasir.upm.edu.my/id/eprint/35948/