Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2
Terminal moieties of most proteins are long known to be disordered and flexible. To unravel the functional role of these regions on the structural stability and biochemical properties of AT2 lipase, four C-terminal end residues, (Ile–Thr–Arg–Lys) which formed a flexible, short tail-like random-coil...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/35925/ http://psasir.upm.edu.my/id/eprint/35925/1/Unscrambling%20the%20effect%20of%20C-terminal%20tail%20deletion%20on%20the%20stability%20of%20a%20cold-adapted%2C%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf |
| _version_ | 1848848190484250624 |
|---|---|
| author | Ahmad Kamarudin, Nor Hafizah Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Ahmad Kamarudin, Nor Hafizah Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Ahmad Kamarudin, Nor Hafizah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Terminal moieties of most proteins are long known to be disordered and flexible. To unravel the functional role of these regions on the structural stability and biochemical properties of AT2 lipase, four C-terminal end residues, (Ile–Thr–Arg–Lys) which formed a flexible, short tail-like random-coil segment were targeted for mutation. Swapping of the tail-like region had resulted in an improved crystallizability and anti-aggregation property along with a slight shift of the thermostability profile. The lipolytic activity of mutant (M386) retained by 43 % compared to its wild-type with 18 % of the remaining activity at 45 °C. In silico analysis conducted at 25 and 45 °C was found to be in accordance to the experimental findings in which the RMSD values of M386 were more stable throughout the total trajectory in comparison to its wild-type. Terminal moieties were also observed to exhibit large movement and flexibility as denoted by high RMSF values at both dynamics. Variation in organic solvent stability property was detected in M386 where the lipolytic activity was stimulated in the presence of 25 % (v/v) of DMSO, isopropanol, and diethyl ether. This may be worth due to changes in the surface charge residues at the mutation point which probably involve in protein–solvent interaction. |
| first_indexed | 2025-11-15T09:30:34Z |
| format | Article |
| id | upm-35925 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:30:34Z |
| publishDate | 2014 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-359252016-09-26T03:57:29Z http://psasir.upm.edu.my/id/eprint/35925/ Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 Ahmad Kamarudin, Nor Hafizah Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar Terminal moieties of most proteins are long known to be disordered and flexible. To unravel the functional role of these regions on the structural stability and biochemical properties of AT2 lipase, four C-terminal end residues, (Ile–Thr–Arg–Lys) which formed a flexible, short tail-like random-coil segment were targeted for mutation. Swapping of the tail-like region had resulted in an improved crystallizability and anti-aggregation property along with a slight shift of the thermostability profile. The lipolytic activity of mutant (M386) retained by 43 % compared to its wild-type with 18 % of the remaining activity at 45 °C. In silico analysis conducted at 25 and 45 °C was found to be in accordance to the experimental findings in which the RMSD values of M386 were more stable throughout the total trajectory in comparison to its wild-type. Terminal moieties were also observed to exhibit large movement and flexibility as denoted by high RMSF values at both dynamics. Variation in organic solvent stability property was detected in M386 where the lipolytic activity was stimulated in the presence of 25 % (v/v) of DMSO, isopropanol, and diethyl ether. This may be worth due to changes in the surface charge residues at the mutation point which probably involve in protein–solvent interaction. Springer 2014-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35925/1/Unscrambling%20the%20effect%20of%20C-terminal%20tail%20deletion%20on%20the%20stability%20of%20a%20cold-adapted%2C%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf Ahmad Kamarudin, Nor Hafizah and Raja Abdul Rahman, Raja Noor Zaliha and Mohamad Ali, Mohd Shukuri and Leow, Thean Chor and Basri, Mahiran and Salleh, Abu Bakar (2014) Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2. Molecular Biotechnology, 56 (8). pp. 747-757. ISSN 1073-6085; ESSN: 1559-0305 http://link.springer.com/article/10.1007%2Fs12033-014-9753-1 10.1007/s12033-014-9753-1 |
| spellingShingle | Ahmad Kamarudin, Nor Hafizah Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Basri, Mahiran Salleh, Abu Bakar Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 |
| title | Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 |
| title_full | Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 |
| title_fullStr | Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 |
| title_full_unstemmed | Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 |
| title_short | Unscrambling the effect of C-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from Staphylococcus epidermidis AT2 |
| title_sort | unscrambling the effect of c-terminal tail deletion on the stability of a cold-adapted, organic solvent stable lipase from staphylococcus epidermidis at2 |
| url | http://psasir.upm.edu.my/id/eprint/35925/ http://psasir.upm.edu.my/id/eprint/35925/ http://psasir.upm.edu.my/id/eprint/35925/ http://psasir.upm.edu.my/id/eprint/35925/1/Unscrambling%20the%20effect%20of%20C-terminal%20tail%20deletion%20on%20the%20stability%20of%20a%20cold-adapted%2C%20organic%20solvent%20stable%20lipase%20from%20Staphylococcus%20epidermidis%20AT2.pdf |