Transgene expression from CpG-reduced lentiviral gene delivery vectors in vitro

QR Code

Transgene expression from CpG-reduced lentiviral gene delivery vectors in vitro

Current viral gene delivery vectors for gene therapy are inefficient due to short-lived transgene expression attributed to the cytosine-phosphate-guanine (CpG) motifs in the transgene. Here we assessed the effects of CpG motif reduction in lentiviral (LV) gene delivery context on the level and durat...

Full description

Bibliographic Details
Main Authors:	Low, Poh Tee, Lai, Mei I., Ngai, Siew Ching, Abdullah, Syahrilnizam
Format:	Article
Published:	Elsevier 2014
Online Access:	http://psasir.upm.edu.my/id/eprint/35891/

Similar Items

Transgene Silencing Following Lentiviral Gene Delivery into Hematopoietic Stem Cells
by: Ngai, Siew Chang
Published: (2011)

Production of lentiviral vector with polycistronic transcripts for reprogramming of mouse fibroblast cells
by: Al Abbar, Akram, et al.
Published: (2018)

CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
by: Hyde, Stephen C., et al.
Published: (2008)

Differential patterns of methylation of the IFN promoter at CpG and non-CpG sites underlie differences in IFN gene expression between human neonatal and adult CD45RO- T-cells
by: White, Greg, et al.
Published: (2002)

Aging of preleukemic thymocytes drives CpG island hypermethylation in T-cell acute lymphoblastic leukemia.
by: Roels, Juliette, et al.
Published: (2020)

Limited expression of non-integrating CpG-free plasmid is associated with increased nucleosome enrichment
by: Habib, Omar, et al.
Published: (2020)

Effects of CpG islands DNA methylation on the human choline kinase alpha promoter activity
by: Sa'dom, Siti Aisyah Faten Mohamed
Published: (2021)

CpG-free plasmid DNA exhibits limited in vitro reporter gene expression due to transcriptional incongruity
by: Habib Rahuman, Muhammad Omar
Published: (2017)

Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
by: Al Abbar, Akram Faisal Mahmaud, et al.
Published: (2018)

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors
by: Ahmed, B.Y., et al.
Published: (2004)

CpG Methylation patterns in the IFN promoter in naive T cells: Variations during Th1 and Th2 differentiation and between atopics and non-atopics
by: White, Greg, et al.
Published: (2006)

The effect of budesonide and 5-azacytidine on the levels of methylation at the CpG islands of human choline kinase alpha (ckα) and beta (ckβ) promoter
by: Rahman, Muhammad Al Bunyamin Abdul
Published: (2017)

Lack of methylation on transgene leads to high level and persistent transgene expression in induced pluripotent stem cells
by: Alhaji, Suleiman Yusuf, et al.
Published: (2020)

Testing the FMR1 promoter for mosaicism in DNA methylation among CpG sites, strands, and cells in FMR1-expressing males with fragile X syndrome
by: Stöger, Reinhard, et al.
Published: (2011)

Evaluation of lentiviral based gene delivery system in adherent and suspension in vitro cell models
by: Izyan Mohd Idris,, et al.
Published: (2024)

Evaluation of lentiviral based gene delivery system in adherent and suspension in vitro cell models
by: Mohd Idris, Izyan, et al.
Published: (2024)

Evaluation of lentiviral based gene delivery system in adherent and suspension in vitro cell models
by: Mohd Idris, Izyan, et al.
Published: (2024)

Modifications Of Nk-92mi Cell Line Through Third Generation Lentiviral Vector
by: Chin, Ding Sheng
Published: (2023)

Generation of mouse induced pluripotent stem cells using polycistronic lentiviral vector in feeder - and serum-free culture
by: Mahmaud, Akram Faisal
Published: (2015)

Generation of hemophilia A mouse induced pluripotent stem cells using polycistronic lentiviral vector in serum- and feeder-free culture conditions
by: M. Sakri, Rozita, et al.
Published: (2016)

Construction of a doxycycline inducible adipogenic lentiviral expression system
by: Liu, Q., et al.
Published: (2013)

Lentiviral tracking of vascular differentiation in bone marrow progenitor cells
by: Schmeckpeper, J., et al.
Published: (2009)

Production of Specialized Transformation Vectors for the Production of Biodegradable Plastics in Transgenic Arabidopsis and Oil Palm
by: Mat Yunus, Abdul Masani
Published: (2006)

Safety profile of dextran-spermine gene delivery vector in mouse lungs
by: Yeo, Wendy Wai Yeng, et al.
Published: (2014)

CPG-based control system for flexible snake robot locomotion
by: Khan, Md. Raisuddin, et al.
Published: (2014)

Integrated cathodic protection (CP) sensor network development of the CP sensor
by: Wold, K., et al.
Published: (2018)

Constructions of expression vectors of polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and transient expression of transgenes in immature oil palm embryos.
by: Ariffin, Norazrin, et al.
Published: (2011)

Optimisation of Delivery of the Ataxia Telangiectasia Mutated Transgene to HeLa Cells Using Glycosaminoglycan Enhanced Transduction
by: Siam, Ala'a
Published: (2021)

Transgenic plants producing polyhydroxyalkanoates
by: Mat Yunus, Abdul Masani, et al.
Published: (2008)

Bio-inspired snake robot locomotion: a CPG-based control approach
by: Billah, Md. Masum, et al.
Published: (2015)

Classification Of Cpg Island And Promoter Regions Using Rare K-Mer Motifs
by: Mohamed Hashim, Ezzeddin Kamil
Published: (2015)

Van Der Pol Central Pattern Generator (VDP-CPG) model for quadruped robot
by: Jasni, Farahiyah, et al.
Published: (2012)

Transgenic organisms (chimeras) and their Islamic evaluation
by: Bouzenita, Anke Sandra, et al.
Published: (2014)

Folate profiling in wild and transgenic rice
by: Abilgos Ramos, Riza
Published: (2010)

Analysis of transgenic wild rice : molecular confirmation of transgene presence and risk assessment for pollen viability / Alireza Saeidi.
by: Saeidi, Alireza
Published: (2011)

Construction of a Transformation Vector Containing Cucumber Mosaic Virus (CMV) Coat Protein (CP) Gene and Development of an Agrobacteriumtransformation Procedure for Chilli
by: Lim, Jee Hian
Published: (2003)

A comprehensive method to generating and identifying transgenic tobacco lines with a single transgene integration locus for functional analysis
by: Hilman, Mohamad Shafek, et al.
Published: (2025)

Synthesis and in vitro delivery of plasmid-encapsulated chitosan nanoparticles in human kidney cancer cells as a vector for gene delivery
by: Cha, Yee Kuen
Published: (2015)

CP sasar sifar jenayah di IPT
by: Sinar Harian,
Published: (2011)

Transgenic nematodes as a model for Parkinson’s disease
by: Bodhicharla, Rakesh Kumar
Published: (2012)