Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology
Optimum conditions for the extraction of pectinesterase from soursop (Anona muricata) have been established. A fractional factorial design and response surface methodology was applied in this study, as a means of improving the method for developing an enzyme extraction procedure. Among the variables...
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| Language: | English English |
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Elsevier
1996
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35793/ http://psasir.upm.edu.my/id/eprint/35793/1/Determination%20of%20optimum%20conditions%20for%20pectinesterase%20extraction%20from%20soursop%20fruit%20%28Anona%20muricata%29%20using%20response%20surface%20methodology.pdf http://psasir.upm.edu.my/id/eprint/35793/7/1-s2.0-0308814695001050-main.pdf |
| _version_ | 1848848153009192960 |
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| author | Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah |
| author_facet | Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah |
| author_sort | Misman, Siti Arbaisah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Optimum conditions for the extraction of pectinesterase from soursop (Anona muricata) have been established. A fractional factorial design and response surface methodology was applied in this study, as a means of improving the method for developing an enzyme extraction procedure. Among the variables tested, NaCl and pH showed greater significant effects, while PVP, EDTA and incubation time seemed to have a lowering effect on the efficiency of pectinesterase extraction from soursop. The maximum enzyme extraction was obtained by using 1.92 M NaCl solution at pH 8.4. |
| first_indexed | 2025-11-15T09:29:58Z |
| format | Article |
| id | upm-35793 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T09:29:58Z |
| publishDate | 1996 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-357932024-08-06T03:05:08Z http://psasir.upm.edu.my/id/eprint/35793/ Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah Optimum conditions for the extraction of pectinesterase from soursop (Anona muricata) have been established. A fractional factorial design and response surface methodology was applied in this study, as a means of improving the method for developing an enzyme extraction procedure. Among the variables tested, NaCl and pH showed greater significant effects, while PVP, EDTA and incubation time seemed to have a lowering effect on the efficiency of pectinesterase extraction from soursop. The maximum enzyme extraction was obtained by using 1.92 M NaCl solution at pH 8.4. Elsevier 1996-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/35793/1/Determination%20of%20optimum%20conditions%20for%20pectinesterase%20extraction%20from%20soursop%20fruit%20%28Anona%20muricata%29%20using%20response%20surface%20methodology.pdf text en http://psasir.upm.edu.my/id/eprint/35793/7/1-s2.0-0308814695001050-main.pdf Misman, Siti Arbaisah and Ali, Asbi and Abdul Hamid, Junainah and Bakar, Jamilah (1996) Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology. Food Chemistry, 55 (3). pp. 289-292. ISSN 0308-8146; ESSN: 1873-7072 http://www.sciencedirect.com/science/article/pii/0308814695001050 10.1016/0308-8146(95)00105-0 |
| spellingShingle | Misman, Siti Arbaisah Ali, Asbi Abdul Hamid, Junainah Bakar, Jamilah Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology |
| title | Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology |
| title_full | Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology |
| title_fullStr | Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology |
| title_full_unstemmed | Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology |
| title_short | Determination of optimum conditions for pectinesterase extraction from soursop fruit (Anona muricata) using response surface methodology |
| title_sort | determination of optimum conditions for pectinesterase extraction from soursop fruit (anona muricata) using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/35793/ http://psasir.upm.edu.my/id/eprint/35793/ http://psasir.upm.edu.my/id/eprint/35793/ http://psasir.upm.edu.my/id/eprint/35793/1/Determination%20of%20optimum%20conditions%20for%20pectinesterase%20extraction%20from%20soursop%20fruit%20%28Anona%20muricata%29%20using%20response%20surface%20methodology.pdf http://psasir.upm.edu.my/id/eprint/35793/7/1-s2.0-0308814695001050-main.pdf |