The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii
Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Instituto Oswaldo Cruz
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/35747/ |
| _version_ | 1848848140676890624 |
|---|---|
| author | Ghafourian, Sobhan Good, Liam Sekawi, Zamberi Awang Hamat, Rukman Soheili, Sara Sadeghifard, Nourkhoda Neela, Vasantha Kumari |
| author_facet | Ghafourian, Sobhan Good, Liam Sekawi, Zamberi Awang Hamat, Rukman Soheili, Sara Sadeghifard, Nourkhoda Neela, Vasantha Kumari |
| author_sort | Ghafourian, Sobhan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems' locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium. |
| first_indexed | 2025-11-15T09:29:46Z |
| format | Article |
| id | upm-35747 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:29:46Z |
| publishDate | 2014 |
| publisher | Instituto Oswaldo Cruz |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-357472016-02-05T00:28:03Z http://psasir.upm.edu.my/id/eprint/35747/ The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii Ghafourian, Sobhan Good, Liam Sekawi, Zamberi Awang Hamat, Rukman Soheili, Sara Sadeghifard, Nourkhoda Neela, Vasantha Kumari Although analysis of toxin-antitoxin (TA) systems can be instructive, to date, there is no information on the prevalence and identity of TA systems based on a large panel of Acinetobacter baumannii clinical isolates. The aim of the current study was to screen for functional TA systems among clinical isolates of A. baumannii and to identify the systems' locations. For this purpose, we screened 85 A. baumannii isolates collected from different clinical sources for the presence of the mazEF, relBE and higBA TA genes. The results revealed that the genes coding for the mazEF TA system were commonly present in all clinical isolates of A. baumannii. Reverse transcriptase-polymerase chain reaction analysis showed that transcripts were produced in the clinical isolates. Our findings showed that TA genes are prevalent, harboured by chromosomes and transcribed within A. baumannii. Hence, activation of the toxin proteins in the mazEF TA system should be investigated further as an effective antibacterial strategy against this bacterium. Instituto Oswaldo Cruz 2014-06 Article NonPeerReviewed Ghafourian, Sobhan and Good, Liam and Sekawi, Zamberi and Awang Hamat, Rukman and Soheili, Sara and Sadeghifard, Nourkhoda and Neela, Vasantha Kumari (2014) The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii. Memorias do Instituto Oswaldo Cruz, 109 (4). pp. 502-505. ISSN 0074-0276; ESSN: 1678-8060 http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0074-02762014000400502&lng=en&nrm=iso&tlng=en 10.1590/0074-0276130601 |
| spellingShingle | Ghafourian, Sobhan Good, Liam Sekawi, Zamberi Awang Hamat, Rukman Soheili, Sara Sadeghifard, Nourkhoda Neela, Vasantha Kumari The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii |
| title | The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii |
| title_full | The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii |
| title_fullStr | The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii |
| title_full_unstemmed | The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii |
| title_short | The mazEF toxin-antitoxin system as a novel antibacterial target in Acinetobacter baumannii |
| title_sort | mazef toxin-antitoxin system as a novel antibacterial target in acinetobacter baumannii |
| url | http://psasir.upm.edu.my/id/eprint/35747/ http://psasir.upm.edu.my/id/eprint/35747/ http://psasir.upm.edu.my/id/eprint/35747/ |