Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments

Cancer-initiating cells [cancer stem cells (CSCs)] in colon cancer cells can be selectively suppressed when they are cultured on Pluronic (nanosegment)-grafted dishes, whereas CSCs are maintained on conventional tissue culture dishes and extracellular matrix-coated dishes. CSCs persist in tumors as...

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Main Authors: Kao, Ta Chun, Lee, Henry Hsin Chung, Higuchi, Akon, Ling, Qing Dong, Yu, Wan Chun, Chou, Yu Hsuan, Wang, Pin Yu, Kumar, S. Suresh, Chang, Yu, Chen, Yung Hung, Chang, Yung, Chen, Da Chung, Hsu, Shih Tien
Format: Article
Published: Wiley 2014
Online Access:http://psasir.upm.edu.my/id/eprint/35537/
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author Kao, Ta Chun
Lee, Henry Hsin Chung
Higuchi, Akon
Ling, Qing Dong
Yu, Wan Chun
Chou, Yu Hsuan
Wang, Pin Yu
Kumar, S. Suresh
Chang, Yu
Chen, Yung Hung
Chang, Yung
Chen, Da Chung
Hsu, Shih Tien
author_facet Kao, Ta Chun
Lee, Henry Hsin Chung
Higuchi, Akon
Ling, Qing Dong
Yu, Wan Chun
Chou, Yu Hsuan
Wang, Pin Yu
Kumar, S. Suresh
Chang, Yu
Chen, Yung Hung
Chang, Yung
Chen, Da Chung
Hsu, Shih Tien
author_sort Kao, Ta Chun
building UPM Institutional Repository
collection Online Access
description Cancer-initiating cells [cancer stem cells (CSCs)] in colon cancer cells can be selectively suppressed when they are cultured on Pluronic (nanosegment)-grafted dishes, whereas CSCs are maintained on conventional tissue culture dishes and extracellular matrix-coated dishes. CSCs persist in tumors as a distinct population and cause relapse and metastasis by giving rise to new tumorigenic clones. The purification or depletion (suppression) of CSCs should be useful for analyzing CSC characteristics and for clinical application. CSCs can be selectively suppressed from colon cancer cells containing adipose-derived stem cells (ADSCs) on Pluronic-grafted dishes, while ADSCs remain on the dishes. ADSCs on Pluronic-grafted dishes after the suppression of the CSCs can differentiate into osteoblasts, chondrocytes, adipocytes, cardiomyocytes, and neuronal cells. The CSCs and ADSCs exhibited different characteristics. The selection of ADSCs was possible on Pluronic-grafted dishes that suppressed the CSCs from the fat tissues of cancer patients (i.e., cell-sorting dishes), which was explained by specific biomedical characteristics of Pluronic. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 463–476, 2014.
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spelling upm-355372016-01-14T03:42:00Z http://psasir.upm.edu.my/id/eprint/35537/ Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments Kao, Ta Chun Lee, Henry Hsin Chung Higuchi, Akon Ling, Qing Dong Yu, Wan Chun Chou, Yu Hsuan Wang, Pin Yu Kumar, S. Suresh Chang, Yu Chen, Yung Hung Chang, Yung Chen, Da Chung Hsu, Shih Tien Cancer-initiating cells [cancer stem cells (CSCs)] in colon cancer cells can be selectively suppressed when they are cultured on Pluronic (nanosegment)-grafted dishes, whereas CSCs are maintained on conventional tissue culture dishes and extracellular matrix-coated dishes. CSCs persist in tumors as a distinct population and cause relapse and metastasis by giving rise to new tumorigenic clones. The purification or depletion (suppression) of CSCs should be useful for analyzing CSC characteristics and for clinical application. CSCs can be selectively suppressed from colon cancer cells containing adipose-derived stem cells (ADSCs) on Pluronic-grafted dishes, while ADSCs remain on the dishes. ADSCs on Pluronic-grafted dishes after the suppression of the CSCs can differentiate into osteoblasts, chondrocytes, adipocytes, cardiomyocytes, and neuronal cells. The CSCs and ADSCs exhibited different characteristics. The selection of ADSCs was possible on Pluronic-grafted dishes that suppressed the CSCs from the fat tissues of cancer patients (i.e., cell-sorting dishes), which was explained by specific biomedical characteristics of Pluronic. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 463–476, 2014. Wiley 2014-04 Article PeerReviewed Kao, Ta Chun and Lee, Henry Hsin Chung and Higuchi, Akon and Ling, Qing Dong and Yu, Wan Chun and Chou, Yu Hsuan and Wang, Pin Yu and Kumar, S. Suresh and Chang, Yu and Chen, Yung Hung and Chang, Yung and Chen, Da Chung and Hsu, Shih Tien (2014) Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments. Journal of Biomedical Materials Research Part B: Applied Biomaterial, 102 (3). pp. 463-476. ISSN 1552-4973; ESSN: 1552-4981 http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33024/abstract 10.1002/jbm.b.33024
spellingShingle Kao, Ta Chun
Lee, Henry Hsin Chung
Higuchi, Akon
Ling, Qing Dong
Yu, Wan Chun
Chou, Yu Hsuan
Wang, Pin Yu
Kumar, S. Suresh
Chang, Yu
Chen, Yung Hung
Chang, Yung
Chen, Da Chung
Hsu, Shih Tien
Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments
title Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments
title_full Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments
title_fullStr Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments
title_full_unstemmed Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments
title_short Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments
title_sort suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments
url http://psasir.upm.edu.my/id/eprint/35537/
http://psasir.upm.edu.my/id/eprint/35537/
http://psasir.upm.edu.my/id/eprint/35537/