A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet
Overweight and obesity are rapidly growing health problem and indeed it has become a strong risk factor in many types of chronic diseases. The aim of this study was to evaluate the anti-obesity effect of saffron extract and its pure bioactive compound, crocin on obesity in in-vivo high fat diet (HFD...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35440/ http://psasir.upm.edu.my/id/eprint/35440/1/A%201H-NMR-based%20metabolomics%20investigation%20on%20the%20effect%20of%20saffron%20extract%20and%20crocin%20on%20rats%20fed%20a%20high%20fat%20diet.pdf |
| _version_ | 1848848055680368640 |
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| author | Ramli, Fatin Najwa Azlan, Azrina |
| author_facet | Ramli, Fatin Najwa Azlan, Azrina |
| author_sort | Ramli, Fatin Najwa |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Overweight and obesity are rapidly growing health problem and indeed it has become a strong risk factor in many types of chronic diseases. The aim of this study was to evaluate the anti-obesity effect of saffron extract and its pure bioactive compound, crocin on obesity in in-vivo high fat diet (HFD) induced model in comparison to orlistat as the positive control. Obese model was successfully established in male Sprague Dawley rats fed by a HFD (40%) for 12 weeks. The obese rats were administered with saffron extract (40 and 80 mg/kg), crocin (40 and 80 mg/kg) and orlistat (20mg/kg) by mixing with HFD for consecutive 8 weeks. Changes in metabolic profiles of serum were determined by using 1H-NMR based metabolomics approach combined with multivariate statistical analysis. The Orthogonal projections to latent structures discriminant analysis (OPLS-DA) on the serum 1H-NMR profiles showed a clear discrimination between obese rats from a normal control rats. This discrimination showed that obese rats serum had higher content of glucose, lactate, β-hydroxybutyrate, alanine and creatinine with lower content of taurine, betaine and trimetylamine N-oxide than those in normal control group. The metabolic profile of serum in obese rat after the administration of saffron extract (80mg/ kg) and crocin (80mg/kg) were deviated from negative control. This results indicated that administration of saffron extract (80mg/ kg) and crocin (80mg/kg) could effectively improve disturbed metabolism in obese rats induced by HFD. These study findings could demonstrated the potential anti-obesity effect of saffron extract and crocin at pre-clinical study. |
| first_indexed | 2025-11-15T09:28:25Z |
| format | Conference or Workshop Item |
| id | upm-35440 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:28:25Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-354402018-04-30T00:55:15Z http://psasir.upm.edu.my/id/eprint/35440/ A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet Ramli, Fatin Najwa Azlan, Azrina Overweight and obesity are rapidly growing health problem and indeed it has become a strong risk factor in many types of chronic diseases. The aim of this study was to evaluate the anti-obesity effect of saffron extract and its pure bioactive compound, crocin on obesity in in-vivo high fat diet (HFD) induced model in comparison to orlistat as the positive control. Obese model was successfully established in male Sprague Dawley rats fed by a HFD (40%) for 12 weeks. The obese rats were administered with saffron extract (40 and 80 mg/kg), crocin (40 and 80 mg/kg) and orlistat (20mg/kg) by mixing with HFD for consecutive 8 weeks. Changes in metabolic profiles of serum were determined by using 1H-NMR based metabolomics approach combined with multivariate statistical analysis. The Orthogonal projections to latent structures discriminant analysis (OPLS-DA) on the serum 1H-NMR profiles showed a clear discrimination between obese rats from a normal control rats. This discrimination showed that obese rats serum had higher content of glucose, lactate, β-hydroxybutyrate, alanine and creatinine with lower content of taurine, betaine and trimetylamine N-oxide than those in normal control group. The metabolic profile of serum in obese rat after the administration of saffron extract (80mg/ kg) and crocin (80mg/kg) were deviated from negative control. This results indicated that administration of saffron extract (80mg/ kg) and crocin (80mg/kg) could effectively improve disturbed metabolism in obese rats induced by HFD. These study findings could demonstrated the potential anti-obesity effect of saffron extract and crocin at pre-clinical study. 2016 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/35440/1/A%201H-NMR-based%20metabolomics%20investigation%20on%20the%20effect%20of%20saffron%20extract%20and%20crocin%20on%20rats%20fed%20a%20high%20fat%20diet.pdf Ramli, Fatin Najwa and Azlan, Azrina (2016) A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet. In: 5th International Conference and Exhibition on Metabolomics, 16-18 May 2016, Osaka, Japan. . https://www.omicsonline.org/2153-0769/2153-0769.C1.032-003.pdf 10.4172/2153-0769.C1.032 |
| spellingShingle | Ramli, Fatin Najwa Azlan, Azrina A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet |
| title | A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet |
| title_full | A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet |
| title_fullStr | A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet |
| title_full_unstemmed | A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet |
| title_short | A 1H-NMR-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet |
| title_sort | 1h-nmr-based metabolomics investigation on the effect of saffron extract and crocin on rats fed a high fat diet |
| url | http://psasir.upm.edu.my/id/eprint/35440/ http://psasir.upm.edu.my/id/eprint/35440/ http://psasir.upm.edu.my/id/eprint/35440/ http://psasir.upm.edu.my/id/eprint/35440/1/A%201H-NMR-based%20metabolomics%20investigation%20on%20the%20effect%20of%20saffron%20extract%20and%20crocin%20on%20rats%20fed%20a%20high%20fat%20diet.pdf |