Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma
A simple and efficient solid-phase membrane tip extraction (SPMTE) was developed using mesoporous silica MCM-41 adsorbent for the determination of three azole antifungal drugs in human plasma prior to high performance liquid chromatography (HPLC). Three azole drugs, namely voriconazole (VRZ), ketoco...
| Main Authors: | , , , , , |
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| Format: | Article |
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Royal Society of Chemistry
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35410/ |
| _version_ | 1848848047035908096 |
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| author | Yahaya, Noorfatimah Sanagi, Mohd Marsin Nur, Hadi Wan Ibrahim, Wan Aini Kamaruzaman, Sazlinda Aboul-Enein, Hassan Y. |
| author_facet | Yahaya, Noorfatimah Sanagi, Mohd Marsin Nur, Hadi Wan Ibrahim, Wan Aini Kamaruzaman, Sazlinda Aboul-Enein, Hassan Y. |
| author_sort | Yahaya, Noorfatimah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A simple and efficient solid-phase membrane tip extraction (SPMTE) was developed using mesoporous silica MCM-41 adsorbent for the determination of three azole antifungal drugs in human plasma prior to high performance liquid chromatography (HPLC). Three azole drugs, namely voriconazole (VRZ), ketoconazole (KTZ) and itraconazole (ITZ), were used as target analytes. The plasma was deproteinized prior to the extraction using methanol–dichloromethane (75 : 25, v/v). Optimized extractions were obtained using the following conditions: conditioning solvent, acetone; extraction time, 15 min; desorption time, 15 min; salt addition, 10% (w/v); pH of sample solution, 8; sample volume, 15 mL and desorption solvent, methanol. A portion of the clean extract (20 μL) was injected into the HPLC-UV system for analysis. Under the optimized conditions, the method demonstrated good linearity with the correlation of determination, r2 ≥ 0.9958 in the concentration range of 60–8000 μg L−1 and good limits of detection in the range of 20–40 μg L−1. The method showed satisfactory precisions with RSDs <16% (n = 3) and high relative recoveries in the range of 82.5–111.0%. The MCM-41-SPMTE method proved to be simple and efficient and requires minimal amounts of organic solvent that supports the green chemistry concept. |
| first_indexed | 2025-11-15T09:28:17Z |
| format | Article |
| id | upm-35410 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:28:17Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-354102016-01-06T03:43:33Z http://psasir.upm.edu.my/id/eprint/35410/ Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma Yahaya, Noorfatimah Sanagi, Mohd Marsin Nur, Hadi Wan Ibrahim, Wan Aini Kamaruzaman, Sazlinda Aboul-Enein, Hassan Y. A simple and efficient solid-phase membrane tip extraction (SPMTE) was developed using mesoporous silica MCM-41 adsorbent for the determination of three azole antifungal drugs in human plasma prior to high performance liquid chromatography (HPLC). Three azole drugs, namely voriconazole (VRZ), ketoconazole (KTZ) and itraconazole (ITZ), were used as target analytes. The plasma was deproteinized prior to the extraction using methanol–dichloromethane (75 : 25, v/v). Optimized extractions were obtained using the following conditions: conditioning solvent, acetone; extraction time, 15 min; desorption time, 15 min; salt addition, 10% (w/v); pH of sample solution, 8; sample volume, 15 mL and desorption solvent, methanol. A portion of the clean extract (20 μL) was injected into the HPLC-UV system for analysis. Under the optimized conditions, the method demonstrated good linearity with the correlation of determination, r2 ≥ 0.9958 in the concentration range of 60–8000 μg L−1 and good limits of detection in the range of 20–40 μg L−1. The method showed satisfactory precisions with RSDs <16% (n = 3) and high relative recoveries in the range of 82.5–111.0%. The MCM-41-SPMTE method proved to be simple and efficient and requires minimal amounts of organic solvent that supports the green chemistry concept. Royal Society of Chemistry 2014 Article PeerReviewed Yahaya, Noorfatimah and Sanagi, Mohd Marsin and Nur, Hadi and Wan Ibrahim, Wan Aini and Kamaruzaman, Sazlinda and Aboul-Enein, Hassan Y. (2014) Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma. Analytical Methods, 6 (10). pp. 3375-3381. ISSN 1759-9660; ESSN: 1759-9679 http://pubs.rsc.org/en/content/articlelanding/2014/ay/c3ay42010h#!divAbstract 10.1039/C3AY42010H |
| spellingShingle | Yahaya, Noorfatimah Sanagi, Mohd Marsin Nur, Hadi Wan Ibrahim, Wan Aini Kamaruzaman, Sazlinda Aboul-Enein, Hassan Y. Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma |
| title | Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma |
| title_full | Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma |
| title_fullStr | Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma |
| title_full_unstemmed | Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma |
| title_short | Solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma |
| title_sort | solid-phase membrane tip extraction combined with liquid chromatography for the determination of azole antifungal drugs in human plasma |
| url | http://psasir.upm.edu.my/id/eprint/35410/ http://psasir.upm.edu.my/id/eprint/35410/ http://psasir.upm.edu.my/id/eprint/35410/ |