RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein
White tail disease (WTD) kills prawn larvae and causes drastic losses to the freshwater prawn (Macrobrachium rosenbergii) industry. The main causative agent of WTD is Macrobrachium rosenbergii nodavirus (MrNV). The N-terminal end of the MrNV capsid protein is very rich in positively charged amino ac...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Society for General Microbiology
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/35287/ |
| _version_ | 1848848012498960384 |
|---|---|
| author | Goh, Zee Hong Syakirin Mohd, Nur Azmina Tan, Soon Guan Bhassu, Subha Tan, Wen Siang |
| author_facet | Goh, Zee Hong Syakirin Mohd, Nur Azmina Tan, Soon Guan Bhassu, Subha Tan, Wen Siang |
| author_sort | Goh, Zee Hong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | White tail disease (WTD) kills prawn larvae and causes drastic losses to the freshwater prawn (Macrobrachium rosenbergii) industry. The main causative agent of WTD is Macrobrachium rosenbergii nodavirus (MrNV). The N-terminal end of the MrNV capsid protein is very rich in positively charged amino acids and is postulated to interact with RNA molecules. N-terminal and internal deletion mutagenesis revealed that the RNA-binding region is located at positions 20–29, where 80 % of amino acids are positively charged. Substitution of all these positively charged residues with alanine abolished the RNA binding. Mutants without the RNA-binding region still assembled into virus-like particles, suggesting that this region is not a part of the capsid assembly domain. This paper is, to the best of our knowledge, the first to report the specific RNA-binding region of MrNV capsid protein. |
| first_indexed | 2025-11-15T09:27:44Z |
| format | Article |
| id | upm-35287 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:27:44Z |
| publishDate | 2014 |
| publisher | Society for General Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-352872015-12-31T04:56:55Z http://psasir.upm.edu.my/id/eprint/35287/ RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein Goh, Zee Hong Syakirin Mohd, Nur Azmina Tan, Soon Guan Bhassu, Subha Tan, Wen Siang White tail disease (WTD) kills prawn larvae and causes drastic losses to the freshwater prawn (Macrobrachium rosenbergii) industry. The main causative agent of WTD is Macrobrachium rosenbergii nodavirus (MrNV). The N-terminal end of the MrNV capsid protein is very rich in positively charged amino acids and is postulated to interact with RNA molecules. N-terminal and internal deletion mutagenesis revealed that the RNA-binding region is located at positions 20–29, where 80 % of amino acids are positively charged. Substitution of all these positively charged residues with alanine abolished the RNA binding. Mutants without the RNA-binding region still assembled into virus-like particles, suggesting that this region is not a part of the capsid assembly domain. This paper is, to the best of our knowledge, the first to report the specific RNA-binding region of MrNV capsid protein. Society for General Microbiology 2014-09 Article NonPeerReviewed Goh, Zee Hong and Syakirin Mohd, Nur Azmina and Tan, Soon Guan and Bhassu, Subha and Tan, Wen Siang (2014) RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein. Journal of General Virology, 95 (9). pp. 1919-1928. ISSN 0022-1317 http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.064014-0 10.1099/vir.0.064014-0 |
| spellingShingle | Goh, Zee Hong Syakirin Mohd, Nur Azmina Tan, Soon Guan Bhassu, Subha Tan, Wen Siang RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein |
| title | RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein |
| title_full | RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein |
| title_fullStr | RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein |
| title_full_unstemmed | RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein |
| title_short | RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein |
| title_sort | rna-binding region of macrobrachium rosenbergii nodavirus capsid protein |
| url | http://psasir.upm.edu.my/id/eprint/35287/ http://psasir.upm.edu.my/id/eprint/35287/ http://psasir.upm.edu.my/id/eprint/35287/ |