Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster
Response surface methodology was used to optimize critical process variables in the production of catechins by an engineered strain of E. coli BL (DE3) carrying an artificial gene cluster of catechin biosynthetic genes. Variables initially identified in literature for metabolic engineering of natura...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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WFL Publisher
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35245/ http://psasir.upm.edu.my/id/eprint/35245/1/Response%20surface%20optimization%20of%20process%20variables%20for%20catechin%20production%20in%20recombinant%20Escherichia%20coli%20BL%20%28DE3%29%20harbouring%20an%20artificial%20gene%20cluster.pdf |
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| author | Umar, Kabir Mustapha Mohammed, Abdulkarim Sabo Radu, Son Abdul Hamid, Azizah Saari, Nazamid |
| author_facet | Umar, Kabir Mustapha Mohammed, Abdulkarim Sabo Radu, Son Abdul Hamid, Azizah Saari, Nazamid |
| author_sort | Umar, Kabir Mustapha |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Response surface methodology was used to optimize critical process variables in the production of catechins by an engineered strain of E. coli BL (DE3) carrying an artificial gene cluster of catechin biosynthetic genes. Variables initially identified in literature for metabolic engineering of natural and non natural products including concentrations of inducer, precursor, temperature and substrate were used to produce optimum yields of catechins. Response optimization of parameters showed the optimum conditions for engineering catechin production from the recombinant E. coli strain to be IPTG (0.9148 mM), glucose (10.0637 g/l), eriodictyol (1.0071 mM) and temperature (27.9560°C) were predicted to produce a response of 0.97178 mg/l with 80.981% desirability. A verification experiment carried out at the optimum produced a yield very close to the predicted value in which the gallated catechins [(-)-epigallocatechin gallate) and (-)-epicatechin gallate] were metabolically engineered for the first time. |
| first_indexed | 2025-11-15T09:27:33Z |
| format | Article |
| id | upm-35245 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:27:33Z |
| publishDate | 2014 |
| publisher | WFL Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-352452018-09-28T08:35:30Z http://psasir.upm.edu.my/id/eprint/35245/ Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster Umar, Kabir Mustapha Mohammed, Abdulkarim Sabo Radu, Son Abdul Hamid, Azizah Saari, Nazamid Response surface methodology was used to optimize critical process variables in the production of catechins by an engineered strain of E. coli BL (DE3) carrying an artificial gene cluster of catechin biosynthetic genes. Variables initially identified in literature for metabolic engineering of natural and non natural products including concentrations of inducer, precursor, temperature and substrate were used to produce optimum yields of catechins. Response optimization of parameters showed the optimum conditions for engineering catechin production from the recombinant E. coli strain to be IPTG (0.9148 mM), glucose (10.0637 g/l), eriodictyol (1.0071 mM) and temperature (27.9560°C) were predicted to produce a response of 0.97178 mg/l with 80.981% desirability. A verification experiment carried out at the optimum produced a yield very close to the predicted value in which the gallated catechins [(-)-epigallocatechin gallate) and (-)-epicatechin gallate] were metabolically engineered for the first time. WFL Publisher 2014 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/35245/1/Response%20surface%20optimization%20of%20process%20variables%20for%20catechin%20production%20in%20recombinant%20Escherichia%20coli%20BL%20%28DE3%29%20harbouring%20an%20artificial%20gene%20cluster.pdf Umar, Kabir Mustapha and Mohammed, Abdulkarim Sabo and Radu, Son and Abdul Hamid, Azizah and Saari, Nazamid (2014) Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster. Journal of Food, Agriculture and Environment, 12 (2). pp. 74-77. ISSN 1459-0255; ESSN: 1459-0263 https://www.wflpublisher.com/Abstract/4363 10.1234/4.2014.4363 |
| spellingShingle | Umar, Kabir Mustapha Mohammed, Abdulkarim Sabo Radu, Son Abdul Hamid, Azizah Saari, Nazamid Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster |
| title | Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster |
| title_full | Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster |
| title_fullStr | Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster |
| title_full_unstemmed | Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster |
| title_short | Response surface optimization of process variables for catechin production in recombinant Escherichia coli BL (DE3) harbouring an artificial gene cluster |
| title_sort | response surface optimization of process variables for catechin production in recombinant escherichia coli bl (de3) harbouring an artificial gene cluster |
| url | http://psasir.upm.edu.my/id/eprint/35245/ http://psasir.upm.edu.my/id/eprint/35245/ http://psasir.upm.edu.my/id/eprint/35245/ http://psasir.upm.edu.my/id/eprint/35245/1/Response%20surface%20optimization%20of%20process%20variables%20for%20catechin%20production%20in%20recombinant%20Escherichia%20coli%20BL%20%28DE3%29%20harbouring%20an%20artificial%20gene%20cluster.pdf |