A Competitive ELISA for quantification of protein a in culture medium
A competitive enzyme-linked immunosorbent assay (ELISA) for quantification of protein A produced by Staphylococcus aureus A676 (a methicillin-resistant strain) was based on competitive binding to rabbit IgG molecules between alkaline phosphatase-labelled protein A and unlabelled protein A. The opt...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Putra Malaysia Press
1996
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| Online Access: | http://psasir.upm.edu.my/id/eprint/3521/ http://psasir.upm.edu.my/id/eprint/3521/1/A_Competitive_ELISA_for_Quantification_of_Protein_A_in_Culture_Mediunt.pdf |
| _version_ | 1848839553491664896 |
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| author | Ali, Abdul Manaf Tahir, Sharifah Abdul Ghani, Baharuddin Ungku Mohsin, Ungku Chulan Ahmad, Ismail |
| author_facet | Ali, Abdul Manaf Tahir, Sharifah Abdul Ghani, Baharuddin Ungku Mohsin, Ungku Chulan Ahmad, Ismail |
| author_sort | Ali, Abdul Manaf |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A competitive enzyme-linked immunosorbent assay (ELISA) for quantification of protein A produced by
Staphylococcus aureus A676 (a methicillin-resistant strain) was based on competitive binding to rabbit IgG
molecules between alkaline phosphatase-labelled protein A and unlabelled protein A. The optimum IgG concentration
required for coating was 2-4 ug/ml. The optimum incubation time for colour development using (p-nitrophenol
phosphate substrate was 20-30 min. The lowest protein A concentration that could be measured using the optimized
competitive ELISA was 20 ng/ml, and the maximum 2 Ug/ml. The amount of protein A produced in brain-heart
infusion medium increased exponentially during log phase of cell growth, reaching a maximum concentration of 22.5
Ug/ml after 15 h cultivation. |
| first_indexed | 2025-11-15T07:13:17Z |
| format | Article |
| id | upm-3521 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:13:17Z |
| publishDate | 1996 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-35212015-09-04T08:05:44Z http://psasir.upm.edu.my/id/eprint/3521/ A Competitive ELISA for quantification of protein a in culture medium Ali, Abdul Manaf Tahir, Sharifah Abdul Ghani, Baharuddin Ungku Mohsin, Ungku Chulan Ahmad, Ismail A competitive enzyme-linked immunosorbent assay (ELISA) for quantification of protein A produced by Staphylococcus aureus A676 (a methicillin-resistant strain) was based on competitive binding to rabbit IgG molecules between alkaline phosphatase-labelled protein A and unlabelled protein A. The optimum IgG concentration required for coating was 2-4 ug/ml. The optimum incubation time for colour development using (p-nitrophenol phosphate substrate was 20-30 min. The lowest protein A concentration that could be measured using the optimized competitive ELISA was 20 ng/ml, and the maximum 2 Ug/ml. The amount of protein A produced in brain-heart infusion medium increased exponentially during log phase of cell growth, reaching a maximum concentration of 22.5 Ug/ml after 15 h cultivation. Universiti Putra Malaysia Press 1996 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3521/1/A_Competitive_ELISA_for_Quantification_of_Protein_A_in_Culture_Mediunt.pdf Ali, Abdul Manaf and Tahir, Sharifah and Abdul Ghani, Baharuddin and Ungku Mohsin, Ungku Chulan and Ahmad, Ismail (1996) A Competitive ELISA for quantification of protein a in culture medium. Pertanika Journal of Tropical Agricultural Science, 19 ((2/3)). pp. 95-102. ISSN 0126-6128 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2019%20(2&3)%20Dec.%201996/00%20JTAS%20Vol.19%20(23)%201996%20(Pg%2095-102).pdf |
| spellingShingle | Ali, Abdul Manaf Tahir, Sharifah Abdul Ghani, Baharuddin Ungku Mohsin, Ungku Chulan Ahmad, Ismail A Competitive ELISA for quantification of protein a in culture medium |
| title | A Competitive ELISA for quantification of protein a in culture medium |
| title_full | A Competitive ELISA for quantification of protein a in culture medium |
| title_fullStr | A Competitive ELISA for quantification of protein a in culture medium |
| title_full_unstemmed | A Competitive ELISA for quantification of protein a in culture medium |
| title_short | A Competitive ELISA for quantification of protein a in culture medium |
| title_sort | competitive elisa for quantification of protein a in culture medium |
| url | http://psasir.upm.edu.my/id/eprint/3521/ http://psasir.upm.edu.my/id/eprint/3521/ http://psasir.upm.edu.my/id/eprint/3521/1/A_Competitive_ELISA_for_Quantification_of_Protein_A_in_Culture_Mediunt.pdf |