Double antibody sandwich enzyme linked Immununosorbent assay kit.
Infectious bursal disease (IBD) is one of the mort threathening and highly contagious viral diseases in chickens in the country causing significant economic losses, estimated more than 72 million per year due to high mortality and immunosuppression. IBD outbreak due to veryvirulent IBD virus (wIBDV)...
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| Format: | Journal |
| Language: | English |
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Research Management Centre, Universiti Putra Malaysia
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/352/ http://psasir.upm.edu.my/id/eprint/352/1/synthesis_11.pdf |
| _version_ | 1848838754987409408 |
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| author | Universiti Putra Malaysia, Research Management Centre |
| author_facet | Universiti Putra Malaysia, Research Management Centre |
| author_sort | Universiti Putra Malaysia, Research Management Centre |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Infectious bursal disease (IBD) is one of the mort threathening and highly contagious viral diseases in chickens in the country causing significant economic losses, estimated more than 72 million per year due to high mortality and immunosuppression. IBD outbreak due to veryvirulent IBD virus (wIBDV) was first reported in Malaysia in 1991. Since then the disease continues to occur despite of vaccination against the disease. IBD can only be controlled and prevented by proper biosecurity and vaccination programmes. Recently, we have successfully developed a highly specific and sensitive Double Antibody Sandwich (DAS) Enzyme linked Immunosorbent Assay (ELISA) Kit for the detection of both the antigen (IBD virus) and antibody against IBD. The kit was developed using local isolate of wIBDV (UPM 971302). The ELISA plate was coated with purified rabbit hyperimmune serum (1gG) prepared in New Zealand White rabbits using the purified virus. |
| first_indexed | 2025-11-15T07:00:35Z |
| format | Journal |
| id | upm-352 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T07:00:35Z |
| publishDate | 2005 |
| publisher | Research Management Centre, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-3522014-11-19T09:02:01Z http://psasir.upm.edu.my/id/eprint/352/ Double antibody sandwich enzyme linked Immununosorbent assay kit. Universiti Putra Malaysia, Research Management Centre Infectious bursal disease (IBD) is one of the mort threathening and highly contagious viral diseases in chickens in the country causing significant economic losses, estimated more than 72 million per year due to high mortality and immunosuppression. IBD outbreak due to veryvirulent IBD virus (wIBDV) was first reported in Malaysia in 1991. Since then the disease continues to occur despite of vaccination against the disease. IBD can only be controlled and prevented by proper biosecurity and vaccination programmes. Recently, we have successfully developed a highly specific and sensitive Double Antibody Sandwich (DAS) Enzyme linked Immunosorbent Assay (ELISA) Kit for the detection of both the antigen (IBD virus) and antibody against IBD. The kit was developed using local isolate of wIBDV (UPM 971302). The ELISA plate was coated with purified rabbit hyperimmune serum (1gG) prepared in New Zealand White rabbits using the purified virus. Research Management Centre, Universiti Putra Malaysia 2005-12 Journal NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/352/1/synthesis_11.pdf Universiti Putra Malaysia, Research Management Centre (2005) Double antibody sandwich enzyme linked Immununosorbent assay kit. Synthesis: R&D Digest of Universiti Putra Malaysia, 11 . ISSN 0127-9394 Research |
| spellingShingle | Research Universiti Putra Malaysia, Research Management Centre Double antibody sandwich enzyme linked Immununosorbent assay kit. |
| title | Double antibody sandwich enzyme linked Immununosorbent assay kit. |
| title_full | Double antibody sandwich enzyme linked Immununosorbent assay kit. |
| title_fullStr | Double antibody sandwich enzyme linked Immununosorbent assay kit. |
| title_full_unstemmed | Double antibody sandwich enzyme linked Immununosorbent assay kit. |
| title_short | Double antibody sandwich enzyme linked Immununosorbent assay kit. |
| title_sort | double antibody sandwich enzyme linked immununosorbent assay kit. |
| topic | Research |
| url | http://psasir.upm.edu.my/id/eprint/352/ http://psasir.upm.edu.my/id/eprint/352/1/synthesis_11.pdf |