Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography

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Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography

Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSki...

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Bibliographic Details
Main Authors:	Sivapragasam, Magaret, Abdullah, Norhafizah
Format:	Article
Language:	English
Published:	Brazilian Society of Chemical Engineering 2015
Online Access:	http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/1/Recovery%20of%20cyclodextrin%20glucanotransferase.pdf

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