Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography
Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSki...
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| Format: | Article |
| Language: | English |
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Brazilian Society of Chemical Engineering
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/1/Recovery%20of%20cyclodextrin%20glucanotransferase.pdf |
| _version_ | 1848847980953600000 |
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| author | Sivapragasam, Magaret Abdullah, Norhafizah |
| author_facet | Sivapragasam, Magaret Abdullah, Norhafizah |
| author_sort | Sivapragasam, Magaret |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSkin dialysis tubing (3.5 MWCO) with an enzyme activity of 147.80 U/mL. Three strategies (A, B and C) were employed for the purification of CGTase using column adsorption chromatography with Ni2+-Sepharose resin. Strategy A employed an elution buffer of 50 mM EDTA, pH 7, Strategy B used 0.1 M imidazole, pH 7 and Strategy C employed 45 mM imidazole pH 7 as the elution buffer. Strategy C was found to be most suitable yielding a total CGTase recovery of 87.04% from an initial activity of 147.80 U/mL. |
| first_indexed | 2025-11-15T09:27:14Z |
| format | Article |
| id | upm-35174 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:27:14Z |
| publishDate | 2015 |
| publisher | Brazilian Society of Chemical Engineering |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-351742016-01-22T03:02:27Z http://psasir.upm.edu.my/id/eprint/35174/ Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography Sivapragasam, Magaret Abdullah, Norhafizah Immobilized metal affinity chromatography (IMAC) was chosen as a method of purification for the recovery of CGTase from E. coli homogenate. E. coli harbouring the Bacillus sp. G1 gene expressed extracellularly secreted CGTase into ampicillin supplied LB broth. Culture was pre-purified using SnakeSkin dialysis tubing (3.5 MWCO) with an enzyme activity of 147.80 U/mL. Three strategies (A, B and C) were employed for the purification of CGTase using column adsorption chromatography with Ni2+-Sepharose resin. Strategy A employed an elution buffer of 50 mM EDTA, pH 7, Strategy B used 0.1 M imidazole, pH 7 and Strategy C employed 45 mM imidazole pH 7 as the elution buffer. Strategy C was found to be most suitable yielding a total CGTase recovery of 87.04% from an initial activity of 147.80 U/mL. Brazilian Society of Chemical Engineering 2015 Article NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/35174/1/Recovery%20of%20cyclodextrin%20glucanotransferase.pdf Sivapragasam, Magaret and Abdullah, Norhafizah (2015) Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography. Brazilian Journal of Chemical Engineering, 32 (1). pp. 43-52. ISSN 0104-6632 http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0104-66322015000100043&lng=en&nrm=iso&tlng=en 10.1590/0104-6632.20150321s00003124 |
| spellingShingle | Sivapragasam, Magaret Abdullah, Norhafizah Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography |
| title | Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography |
| title_full | Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography |
| title_fullStr | Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography |
| title_full_unstemmed | Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography |
| title_short | Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography |
| title_sort | recovery of cyclodextrin glucanotransferase (cgtase) using immobilized metal chelating affinity chromatography |
| url | http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/ http://psasir.upm.edu.my/id/eprint/35174/1/Recovery%20of%20cyclodextrin%20glucanotransferase.pdf |