Purification of glutathione S-transferase (GST) using mixed mode chromatography

QR Code

Purification of glutathione S-transferase (GST) using mixed mode chromatography

A simple and small scale laboratory method to compare between ultrasonication, glass bead shaking and chemical lysis were evaluated for the release of recombinant Glutathione S-Transferase (GST) from Escherichia coli. Since the protein Glutathione S-Transferase is expressed intracellularly, cell dis...

Full description

Bibliographic Details
Main Authors:	M., Sivapragasam, Abdullah, Norhafizah
Format:	Article
Published:	ESRSA Publications 2014
Online Access:	http://psasir.upm.edu.my/id/eprint/35117/

Similar Items

Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4
by: Kong, Christina Wen Hui, et al.
Published: (2022)

Bioinformatic analysis and purification of glutathione transferase (GST) from Pseudomonas sp. UW4
by: Kong, Christina Wen Hui, et al.
Published: (2022)

Purification and characterization of isoforms of glutathione S-transferase (GST) from Acinetobacter Calcoaceticus Y1 / Chee Chin Soon
by: Chee, Chin Soon
Published: (2014)

Purification of glutathione Transferases (GSTs) from identified Rhizospheric bacteria
by: Khanom Simarani,, et al.
Published: (2016)

Isolation and purification of glutathione transferases from Donax sp. / Norfarhan Mohd Assa'ad
by: Norfarhan, Mohd Assa'ad
Published: (2011)

Isolation and purification of glutathione S-transferases (GSTs) from Orbicularia orbiculata / Marini binti Ibrahim
by: Ibrahim, Marini
Published: (2012)

Isolation and purification of glutathione s-transferases from donax sp / Norfarhan Mohd Assa’Ad
by: Mohd Assa’Ad, Norfarhan
Published: (2011)

Study on local plants as source of inhibitors to human glutathione s-transferase π (GST-P) / Phang Wai Mei
by: Phang, Wai Mei
Published: (2013)

Isolation and characterization of EgGST, a glutathione S-transferase protein transcript in oil palm (Elaeis guineensis Jacq.)
by: Conie, Toh, et al.
Published: (2015)

Differential expression of glutathione S-transferase (GST) by adult Heligmosomoides polygyrus during primary infection in fast and slow responding hosts
by: Brophy, Peter M., et al.
Published: (1995)

Dietary Isothiocyanate (ITC), Glutathione S-Transferases (GST) Polymorphisms and risk of Oral Squamous Cell Carcinoma (OSCC) / Karen Ng Lee Peng
by: Ng, Karen Lee Peng
Published: (2007)

Synthesis, properties and application of titanium dioxide doped with nitrogen. Its effectiveness on photo degradation glutathione-S transferase GST enzymes pupae instar of Aedes aegypti
by: AlSalih, Moatasem, et al.
Published: (2021)

Partial Purification and Characterization of Glutathione S-Transferase from the Livers of Malaysian Catfish (Clarias Batrachus) and Malaysian Red Tilapia (Oreochromis Sp.)
by: Chin, Chee Meng
Published: (2005)

Partial Purification and Characterization of Glutathione S-Transferases from Kedah-Kelantan Cattle (Bos Indicus) and Water Buffalo (Bubalus Bubalis) Liver
by: Saleh Huddin, Lailatul Jumaiyah
Published: (2006)

Insecticide toxicity, glutathione transferases and carboxylesterase activities in the larva of the Aedes mosquito
by: Shamaan, N.A., et al.
Published: (1993)

Purification of rabbit polyclonal immunoglobulin G with ammonium sulphate precipitation and mixed-mode chromatography
by: Mariam, S. H. S, et al.
Published: (2015)

The effect of tocotrienol-rich fraction on the expression of glutathione S-transferase isoenzymes in mice liver
by: Ahmed Atia,, et al.
Published: (2018)

Lack of association of glutathione S-transferase gene polymorphisms in Iranian prostate cancer subjects
by: Basir, Sahar Ansari, et al.
Published: (2009)

γ-Glutamyl transpeptidase, glutathione S-transferase, alkaline phosphatase, and glutathione levels during different stages of chemically induced hepatocarcinogenesis in the rat
by: Makpol, Suzana, et al.
Published: (1996)

Bioinformatics and functional analysis of glutathione s-transferases of Pseudomonas putida KT2440 / Nasiru Abdullahi
by: Nasiru , Abdullahi
Published: (2018)

Glutathione s-transferase: an overview on distribution of GSTM1 and GSTT1 polymorphisms in Malaysian and other populations
by: Wan Rashidi, Wan Nooremira, et al.
Published: (2019)

Insecticidal activity of phenylpropanoids from Piper sarmentosum and their interactions with glutathione S-transferase from adult mosquitoes
by: Hematpoor, Arshia, et al.
Published: (2025)

Glutathione S-transferase and gamma-glutamyl transpeptidase activities in cultured rat hepatocytes treated with tocotrienol and tocopherol
by: Ong, F.B., et al.
Published: (1993)

Recovery of cyclodextrin glucanotransferase (CGTase) using immobilized metal chelating affinity chromatography
by: Sivapragasam, Magaret, et al.
Published: (2015)

Vitamin E, glutathione S-transferase and γ-glutamyl transpeptidase activities in cultured hepatocytes of rats treated with carcinogens
by: Ong, F.B., et al.
Published: (1994)

Cloning and biochemical characterization of CG16936, a putative glutathione transferase in Drosophila Melanogaster / Tanusya Murali
by: Murali, Tanusya
Published: (2014)

In utero smoke exposure and role of maternal and infant glutathione S-transferase genes on airway responsiveness and lung function in infancy
by: Murdzoska, J., et al.
Published: (2010)

Analysis of glutathione S-transferase (M1, T1 and P1) gene polymorphisms in Iranian prostate cancer subjects
by: Basir, Sahar Ansari, et al.
Published: (2010)

Mixed-mode interaction membrane chromatography for protein fractionation
by: Nur Farahain, Khusnun
Published: (2012)

Effects of pterostilbene on activities and protein expression of cytochrome P450 1A1 (CYP1A1) and glutathione s-transferase (GST) in benzo[a]pyrene-induced HT-29 colorectal cancer cell line
by: Ahmad Rohi Ghazali,, et al.
Published: (2018)

Study on the biochemical function of recombinant delta class drosophila melanogaster glutathione s-transferase 3 (DMGSTD3) / Kithalakshmi Vignesvaran
by: Kithalakshmi, Vignesvaran
Published: (2017)

Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease
by: Etemad, Ali, et al.
Published: (2016)

Evaluation of glutathione S-transferases expression as biomarkers by heavy metals in Geloina expansa from Sepang Besar River, Selangor, Malaysia
by: Wakid, Sarini Ahmad, et al.
Published: (2024)

Development of Bioaffinity Chromatography for Uricase Purification
by: Salleh, Abu Bakar
Published: (1980)

Cellular total lipid peroxidation, and glutathione S transferase levels in larvae and pupae of Aedes Aegypti with catalysts preparation of Mg-doped tio2 nanoparticles
by: AlSalih, Moatasem, et al.
Published: (2021)

Blood heavy metals (arsenic, cadmium and lead) concentration and genetic polymorphism of glutathione S-transferase genes among adults in coastal area of Melaka, Malaysia
by: Mohd Noor, Farrah Atiqah, et al.
Published: (2022)

Fabrication of ceramic membrane chromatography for biologics purification
by: Mel, Maizirwan, et al.
Published: (2009)

Fabrication of ceramic membrane chromatography for biologics purification
by: Mel, Maizirwan, et al.
Published: (2011)

Comparative biochemical characterization of Drosophila Melanogaster epsilon class glutathione s-transferase, DmGSTE6 and DmGSTE7 / Vennobaahshini a/p Venu
by: Venu, Vennobaahshini
Published: (2015)

The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis
by: Mehdi, Wesen Adel, et al.
Published: (2019)