Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees
Separation of proteins based on the physicochemical properties with different molecular weight and isoelectric points would be more accurate. In the current research, the 45-day-old seedlings were treated with 0 (control) and 12 dS m−1 of sodium chloride in the hydroponic system. After 15 days of sa...
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| Language: | English |
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Public Library of Science
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/35097/ http://psasir.upm.edu.my/id/eprint/35097/1/1234.PDF |
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| author | Talei, Daryush Valdiani, Alireza Yusop, Mohd Rafii Mahmood, Maziah |
| author_facet | Talei, Daryush Valdiani, Alireza Yusop, Mohd Rafii Mahmood, Maziah |
| author_sort | Talei, Daryush |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Separation of proteins based on the physicochemical properties with different molecular weight and isoelectric points would be more accurate. In the current research, the 45-day-old seedlings were treated with 0 (control) and 12 dS m−1 of sodium chloride in the hydroponic system. After 15 days of salt exposure, the total protein of the fresh leaves and roots was extracted and analyzed using two-dimensional electrophoresis system (2-DE). The analysis led to the detection of 32 induced proteins (19 proteins in leaf and 13 proteins in the root) as well as 12 upregulated proteins (four proteins in leaf and eight proteins in the root) in the salt-treated plants. Of the 44 detected proteins, 12 were sequenced, and three of them matched with superoxide dismutase, ascorbate peroxidase and ribulose-1, 5-bisphosphate oxygenase whereas the rest remained unknown. The three known proteins associate with plants response to environmental stresses and could represent the general stress proteins in the present study too. In addition, the proteomic feedback of different accessions of A. paniculata to salt stress can potentially be used to breed salt-tolerant varieties of the herb. |
| first_indexed | 2025-11-15T09:26:53Z |
| format | Article |
| id | upm-35097 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:26:53Z |
| publishDate | 2014 |
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| spelling | upm-350972019-10-01T00:55:23Z http://psasir.upm.edu.my/id/eprint/35097/ Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees Talei, Daryush Valdiani, Alireza Yusop, Mohd Rafii Mahmood, Maziah Separation of proteins based on the physicochemical properties with different molecular weight and isoelectric points would be more accurate. In the current research, the 45-day-old seedlings were treated with 0 (control) and 12 dS m−1 of sodium chloride in the hydroponic system. After 15 days of salt exposure, the total protein of the fresh leaves and roots was extracted and analyzed using two-dimensional electrophoresis system (2-DE). The analysis led to the detection of 32 induced proteins (19 proteins in leaf and 13 proteins in the root) as well as 12 upregulated proteins (four proteins in leaf and eight proteins in the root) in the salt-treated plants. Of the 44 detected proteins, 12 were sequenced, and three of them matched with superoxide dismutase, ascorbate peroxidase and ribulose-1, 5-bisphosphate oxygenase whereas the rest remained unknown. The three known proteins associate with plants response to environmental stresses and could represent the general stress proteins in the present study too. In addition, the proteomic feedback of different accessions of A. paniculata to salt stress can potentially be used to breed salt-tolerant varieties of the herb. Public Library of Science 2014-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/35097/1/1234.PDF Talei, Daryush and Valdiani, Alireza and Yusop, Mohd Rafii and Mahmood, Maziah (2014) Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees. PLOS ONE, 9 (11). art. no. e112907. pp. 1-10. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112907 10.1371/journal.pone.0112907 |
| spellingShingle | Talei, Daryush Valdiani, Alireza Yusop, Mohd Rafii Mahmood, Maziah Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees |
| title | Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees |
| title_full | Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees |
| title_fullStr | Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees |
| title_full_unstemmed | Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees |
| title_short | Proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant Andrographis paniculata Nees |
| title_sort | proteomic analysis of the salt-responsive leaf and root proteins in the anticancer plant andrographis paniculata nees |
| url | http://psasir.upm.edu.my/id/eprint/35097/ http://psasir.upm.edu.my/id/eprint/35097/ http://psasir.upm.edu.my/id/eprint/35097/ http://psasir.upm.edu.my/id/eprint/35097/1/1234.PDF |