Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation
The energy-salvaging capacity of the gut microbiota from dietary ingredients has been proposed as a contributing factor for the development of obesity. This knowledge generated interest in the use of non-digestible dietary ingredients such as prebiotics to manipulate host energy homeostasis. In the...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34972/ http://psasir.upm.edu.my/id/eprint/34972/1/Potential%20of%20novel%20dextran%20oligosaccharides%20as%20prebiotics%20for%20obesity%20management%20through%20in%20vitro%20experimentation.pdf |
| _version_ | 1848847923113099264 |
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| author | Sarbini, Shahrul Razid Kolida, Sofia Deaville, Eddie R. Gibson, Glenn R. Rastall, Robert A. |
| author_facet | Sarbini, Shahrul Razid Kolida, Sofia Deaville, Eddie R. Gibson, Glenn R. Rastall, Robert A. |
| author_sort | Sarbini, Shahrul Razid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The energy-salvaging capacity of the gut microbiota from dietary ingredients has been proposed as a contributing factor for the development of obesity. This knowledge generated interest in the use of non-digestible dietary ingredients such as prebiotics to manipulate host energy homeostasis. In the present study, the in vitro response of obese human faecal microbiota to novel oligosaccharides was investigated. Dextrans of various molecular weights and degrees of branching were fermented with the faecal microbiota of healthy obese adults in pH-controlled batch cultures. Changes in bacterial populations were monitored using fluorescent in situ hybridisation and SCFA concentrations were analysed by HPLC. The rate of gas production and total volume of gas produced were also determined. In general, the novel dextrans and inulin increased the counts of bifidobacteria. Some of the dextrans were able to alter the composition of the obese human microbiota by increasing the counts of Bacteroides–Prevotella and decreasing those of Faecalibacterium prausnitzii and Ruminococcus bromii/R. flavefaciens. Considerable increases in SCFA concentrations were observed in response to all substrates. Gas production rates were similar during the fermentation of all dextrans, but significantly lower than those during the fermentation of inulin. Lower total gas production and shorter time to attain maximal gas production were observed during the fermentation of the linear 1 kDa dextran than during the fermentation of the other dextrans. The efficacy of bifidobacteria to ferment dextrans relied on the molecular weight and not on the degree of branching. In conclusion, there are no differences in the profiles between the obese and lean human faecal fermentations of dextrans. |
| first_indexed | 2025-11-15T09:26:19Z |
| format | Article |
| id | upm-34972 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:26:19Z |
| publishDate | 2014 |
| publisher | Cambridge University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-349722016-01-27T03:17:54Z http://psasir.upm.edu.my/id/eprint/34972/ Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation Sarbini, Shahrul Razid Kolida, Sofia Deaville, Eddie R. Gibson, Glenn R. Rastall, Robert A. The energy-salvaging capacity of the gut microbiota from dietary ingredients has been proposed as a contributing factor for the development of obesity. This knowledge generated interest in the use of non-digestible dietary ingredients such as prebiotics to manipulate host energy homeostasis. In the present study, the in vitro response of obese human faecal microbiota to novel oligosaccharides was investigated. Dextrans of various molecular weights and degrees of branching were fermented with the faecal microbiota of healthy obese adults in pH-controlled batch cultures. Changes in bacterial populations were monitored using fluorescent in situ hybridisation and SCFA concentrations were analysed by HPLC. The rate of gas production and total volume of gas produced were also determined. In general, the novel dextrans and inulin increased the counts of bifidobacteria. Some of the dextrans were able to alter the composition of the obese human microbiota by increasing the counts of Bacteroides–Prevotella and decreasing those of Faecalibacterium prausnitzii and Ruminococcus bromii/R. flavefaciens. Considerable increases in SCFA concentrations were observed in response to all substrates. Gas production rates were similar during the fermentation of all dextrans, but significantly lower than those during the fermentation of inulin. Lower total gas production and shorter time to attain maximal gas production were observed during the fermentation of the linear 1 kDa dextran than during the fermentation of the other dextrans. The efficacy of bifidobacteria to ferment dextrans relied on the molecular weight and not on the degree of branching. In conclusion, there are no differences in the profiles between the obese and lean human faecal fermentations of dextrans. Cambridge University Press 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34972/1/Potential%20of%20novel%20dextran%20oligosaccharides%20as%20prebiotics%20for%20obesity%20management%20through%20in%20vitro%20experimentation.pdf Sarbini, Shahrul Razid and Kolida, Sofia and Deaville, Eddie R. and Gibson, Glenn R. and Rastall, Robert A. (2014) Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation. British Journal of Nutrition, 112 (8). pp. 1303-1304. ISSN 0007-1145; ESSN: 1475-2662 http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9381677&fulltextType=RA&fileId=S0007114514002177 10.1017/S0007114514002177 |
| spellingShingle | Sarbini, Shahrul Razid Kolida, Sofia Deaville, Eddie R. Gibson, Glenn R. Rastall, Robert A. Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation |
| title | Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation |
| title_full | Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation |
| title_fullStr | Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation |
| title_full_unstemmed | Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation |
| title_short | Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation |
| title_sort | potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation |
| url | http://psasir.upm.edu.my/id/eprint/34972/ http://psasir.upm.edu.my/id/eprint/34972/ http://psasir.upm.edu.my/id/eprint/34972/ http://psasir.upm.edu.my/id/eprint/34972/1/Potential%20of%20novel%20dextran%20oligosaccharides%20as%20prebiotics%20for%20obesity%20management%20through%20in%20vitro%20experimentation.pdf |