Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers

The genetic variation of nine varieties of cultivated mungbean (Vigna radiata) and three local populations of wild Vigna (V. trinervia) were evaluated in this study using RAPD markers. A total of 65 scorable DNA fragments ranging in size from 173-1,500 bp were obtained from the PCR amplification us...

Full description

Bibliographic Details
Main Authors: S. V., Kumar, T. M., Saw, Tan, Soon Guan, S. C., Quah
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2003
Online Access:http://psasir.upm.edu.my/id/eprint/3497/
http://psasir.upm.edu.my/id/eprint/3497/1/Variation_of_Cultivated_Mungbean_and_WIld_Vigna_as_Revealed.pdf
_version_ 1848839547134148608
author S. V., Kumar
T. M., Saw
Tan, Soon Guan
S. C., Quah
author_facet S. V., Kumar
T. M., Saw
Tan, Soon Guan
S. C., Quah
author_sort S. V., Kumar
building UPM Institutional Repository
collection Online Access
description The genetic variation of nine varieties of cultivated mungbean (Vigna radiata) and three local populations of wild Vigna (V. trinervia) were evaluated in this study using RAPD markers. A total of 65 scorable DNA fragments ranging in size from 173-1,500 bp were obtained from the PCR amplification usingfive RAPD primers of which 95.38% were polymorphic. Cluster analysis revealed two major groups in which the first group consists of the nine varieties ofV. radiata, while the second group includes the three populations ofV. trinervia. This information is useful for plant breeders to make informed decisions in an effort to devise breeding or crossbreeding programmes for the development of the crop.
first_indexed 2025-11-15T07:13:11Z
format Article
id upm-3497
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T07:13:11Z
publishDate 2003
publisher Universiti Putra Malaysia Press
recordtype eprints
repository_type Digital Repository
spelling upm-34972015-09-11T01:05:40Z http://psasir.upm.edu.my/id/eprint/3497/ Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers S. V., Kumar T. M., Saw Tan, Soon Guan S. C., Quah The genetic variation of nine varieties of cultivated mungbean (Vigna radiata) and three local populations of wild Vigna (V. trinervia) were evaluated in this study using RAPD markers. A total of 65 scorable DNA fragments ranging in size from 173-1,500 bp were obtained from the PCR amplification usingfive RAPD primers of which 95.38% were polymorphic. Cluster analysis revealed two major groups in which the first group consists of the nine varieties ofV. radiata, while the second group includes the three populations ofV. trinervia. This information is useful for plant breeders to make informed decisions in an effort to devise breeding or crossbreeding programmes for the development of the crop. Universiti Putra Malaysia Press 2003 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3497/1/Variation_of_Cultivated_Mungbean_and_WIld_Vigna_as_Revealed.pdf S. V., Kumar and T. M., Saw and Tan, Soon Guan and S. C., Quah (2003) Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers. Pertanika Journal of Tropical Agricultural Science, 26 (2). pp. 123-129. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2026%20(2)%20Sep.%202003/08%20JTAS%20Vol.26%20(2)%202003%20(Pg%20123-129).pdf
spellingShingle S. V., Kumar
T. M., Saw
Tan, Soon Guan
S. C., Quah
Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers
title Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers
title_full Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers
title_fullStr Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers
title_full_unstemmed Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers
title_short Variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic DNA markers
title_sort variation of cultivated mungbean and wild vigna as revealed by random amplified polymorphic dna markers
url http://psasir.upm.edu.my/id/eprint/3497/
http://psasir.upm.edu.my/id/eprint/3497/
http://psasir.upm.edu.my/id/eprint/3497/1/Variation_of_Cultivated_Mungbean_and_WIld_Vigna_as_Revealed.pdf