Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model
Autogenous bone graft is the gold standard for fusion procedure. However, pain at donor site and inconsistent outcome have left a surgeon to venture into some other technique for spinal fusion. The objective of this study was to determine whether osteogenesis induced bone marrow stem cells with the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34966/ http://psasir.upm.edu.my/id/eprint/34966/1/Posterolateral%20spinal%20fusion%20with%20ostegenesis%20induced%20BMSC%20seeded%20TCP.pdf |
| _version_ | 1848847921375608832 |
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| author | Sulaiman, Shamsul Tan, Kok Keong Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah |
| author_facet | Sulaiman, Shamsul Tan, Kok Keong Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah |
| author_sort | Sulaiman, Shamsul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Autogenous bone graft is the gold standard for fusion procedure. However, pain at donor site and inconsistent outcome have left a surgeon to venture into some other technique for spinal fusion. The objective of this study was to determine whether osteogenesis induced bone marrow stem cells with the combination of ceramics granules (HA or TCP/HA), and fibrin could serve as an alternative to generate spinal fusion. The sheep's bone marrow mesenchymal stem cells (BMSCs) were aspirated form iliac crest and cultured for several passages until confluence. BMSCs were trypsinized and seeded on hydroxyapatite scaffold (HA) and tricalcium phosphate/hydroxyapatite (TCP/HA) for further osteogenic differentiation in the osteogenic medium one week before implantation. Six adult sheep underwent three-level, bilateral, posterolateral intertransverse process fusions at L1–L6. Three fusion sites in each animal were assigned to three treatments: (a) HA constructs group/L1–L2, (b) TCP/HA constructs group/L2–L3, and (c) autogenous bone graft group/L5–L6. The spinal fusion segments were evaluated using radiography, manual palpation, histological analysis and scanning electron microscopy (SEM) 12 weeks post implantation. The TCP/HA constructs achieved superior lumbar intertransverse fusion compared to HA construct but autogenous bone graft still produced the best fusion among all. |
| first_indexed | 2025-11-15T09:26:17Z |
| format | Article |
| id | upm-34966 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:26:17Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-349662016-02-03T03:04:36Z http://psasir.upm.edu.my/id/eprint/34966/ Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model Sulaiman, Shamsul Tan, Kok Keong Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah Autogenous bone graft is the gold standard for fusion procedure. However, pain at donor site and inconsistent outcome have left a surgeon to venture into some other technique for spinal fusion. The objective of this study was to determine whether osteogenesis induced bone marrow stem cells with the combination of ceramics granules (HA or TCP/HA), and fibrin could serve as an alternative to generate spinal fusion. The sheep's bone marrow mesenchymal stem cells (BMSCs) were aspirated form iliac crest and cultured for several passages until confluence. BMSCs were trypsinized and seeded on hydroxyapatite scaffold (HA) and tricalcium phosphate/hydroxyapatite (TCP/HA) for further osteogenic differentiation in the osteogenic medium one week before implantation. Six adult sheep underwent three-level, bilateral, posterolateral intertransverse process fusions at L1–L6. Three fusion sites in each animal were assigned to three treatments: (a) HA constructs group/L1–L2, (b) TCP/HA constructs group/L2–L3, and (c) autogenous bone graft group/L5–L6. The spinal fusion segments were evaluated using radiography, manual palpation, histological analysis and scanning electron microscopy (SEM) 12 weeks post implantation. The TCP/HA constructs achieved superior lumbar intertransverse fusion compared to HA construct but autogenous bone graft still produced the best fusion among all. Elsevier 2014-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34966/1/Posterolateral%20spinal%20fusion%20with%20ostegenesis%20induced%20BMSC%20seeded%20TCP.pdf Sulaiman, Shamsul and Tan, Kok Keong and Chen, Hui Cheng and Saim, Aminuddin and Idrus, Ruszymah (2014) Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model. Tissue and Cell, 46 (2). pp. 152-158. ISSN 0040-8166; ESSN: 1532-3072 http://www.sciencedirect.com/science/article/pii/S0040816614000123 10.1016/j.tice.2014.02.001 |
| spellingShingle | Sulaiman, Shamsul Tan, Kok Keong Chen, Hui Cheng Saim, Aminuddin Idrus, Ruszymah Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model |
| title | Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model |
| title_full | Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model |
| title_fullStr | Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model |
| title_full_unstemmed | Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model |
| title_short | Posterolateral spinal fusion with ostegenesis induced BMSC seeded TCP/HA in a sheep model |
| title_sort | posterolateral spinal fusion with ostegenesis induced bmsc seeded tcp/ha in a sheep model |
| url | http://psasir.upm.edu.my/id/eprint/34966/ http://psasir.upm.edu.my/id/eprint/34966/ http://psasir.upm.edu.my/id/eprint/34966/ http://psasir.upm.edu.my/id/eprint/34966/1/Posterolateral%20spinal%20fusion%20with%20ostegenesis%20induced%20BMSC%20seeded%20TCP.pdf |