Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae
Pichia pastoris was known to be a good expression system in producing various heterologous proteins. The gene encoding thermostable Tl lipase from Geobacillus zalihae was cloned into pPICZαB and expressed in P. pastoris strains (GS115, X-33 and KM71H) under regulation of alcohol oxidase promoter. T...
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Global Science and Technology Forum
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34942/ http://psasir.upm.edu.my/id/eprint/34942/1/Pichia%20pastoris%20as%20a%20host%20to%20overexpress%20the%20thermostable%20T1%20lipase%20from%20Geobacillus%20zalihae.pdf |
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| author | Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Basri, Mahiran |
| author_facet | Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Basri, Mahiran |
| author_sort | Oslan, Siti Nurbaya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pichia pastoris was known to be a good expression system in producing various heterologous proteins. The gene encoding thermostable Tl lipase from Geobacillus zalihae was cloned into pPICZαB and expressed in P. pastoris strains (GS115, X-33 and KM71H) under regulation of alcohol oxidase promoter. The expression of the gene in Escherichia coli system showed low expression level. Therefore, this study would highlight on the overexpression of the T1 lipase in yeast extracellularly. Recombinant X-33/pPICZαB/T1-2 (XPB2), GS115/pPICZαB/T1-5 (GPB5) and KM71H/pPICZαB/T1-7 (KPB7) were chosen for optimization in shake flask. Optimization strategies showed that these recombinants preferred YPTM medium with initial induction OD600nm = 7 cell biomass and 2% (v/v) methanol to provide optimal expression conditions. Hyper-resistant transformants at 3000 ug/mL zeocin gave better expression than 100 ug/mL zeocin selection. Time course study by using different inocula age showed that OD600nm = 7 expressed lipase at the highest level. The highest expression level was attained with GPB5 (88 U/ml), XPB2 (81 U/ml) and KPB7 (51 U/ml). Western Blot analysis confirmed that the molecular mass of recombinant Tl lipase was 45 kDa. In conclusion, thermostable Tl lipase was successfully overexpressed by using secretory P. pastoris system with two-fold higher than E. coli system. |
| first_indexed | 2025-11-15T09:26:11Z |
| format | Article |
| id | upm-34942 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:26:11Z |
| publishDate | 2014 |
| publisher | Global Science and Technology Forum |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-349422016-09-28T04:01:24Z http://psasir.upm.edu.my/id/eprint/34942/ Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Basri, Mahiran Pichia pastoris was known to be a good expression system in producing various heterologous proteins. The gene encoding thermostable Tl lipase from Geobacillus zalihae was cloned into pPICZαB and expressed in P. pastoris strains (GS115, X-33 and KM71H) under regulation of alcohol oxidase promoter. The expression of the gene in Escherichia coli system showed low expression level. Therefore, this study would highlight on the overexpression of the T1 lipase in yeast extracellularly. Recombinant X-33/pPICZαB/T1-2 (XPB2), GS115/pPICZαB/T1-5 (GPB5) and KM71H/pPICZαB/T1-7 (KPB7) were chosen for optimization in shake flask. Optimization strategies showed that these recombinants preferred YPTM medium with initial induction OD600nm = 7 cell biomass and 2% (v/v) methanol to provide optimal expression conditions. Hyper-resistant transformants at 3000 ug/mL zeocin gave better expression than 100 ug/mL zeocin selection. Time course study by using different inocula age showed that OD600nm = 7 expressed lipase at the highest level. The highest expression level was attained with GPB5 (88 U/ml), XPB2 (81 U/ml) and KPB7 (51 U/ml). Western Blot analysis confirmed that the molecular mass of recombinant Tl lipase was 45 kDa. In conclusion, thermostable Tl lipase was successfully overexpressed by using secretory P. pastoris system with two-fold higher than E. coli system. Global Science and Technology Forum 2014-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34942/1/Pichia%20pastoris%20as%20a%20host%20to%20overexpress%20the%20thermostable%20T1%20lipase%20from%20Geobacillus%20zalihae.pdf Oslan, Siti Nurbaya and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha and Leow, Adam Thean Chor and Basri, Mahiran (2014) Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae. GSTF Journal of Biosciences, 3 (1). pp. 7-17. ISSN 2251-3140; ESSN: 2251-3159 http://dl6.globalstf.org/index.php/jbio/article/view/1002 10.5176/2251-3140_3.1.45 |
| spellingShingle | Oslan, Siti Nurbaya Salleh, Abu Bakar Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Basri, Mahiran Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae |
| title | Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae |
| title_full | Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae |
| title_fullStr | Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae |
| title_full_unstemmed | Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae |
| title_short | Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae |
| title_sort | pichia pastoris as a host to overexpress the thermostable t1 lipase from geobacillus zalihae |
| url | http://psasir.upm.edu.my/id/eprint/34942/ http://psasir.upm.edu.my/id/eprint/34942/ http://psasir.upm.edu.my/id/eprint/34942/ http://psasir.upm.edu.my/id/eprint/34942/1/Pichia%20pastoris%20as%20a%20host%20to%20overexpress%20the%20thermostable%20T1%20lipase%20from%20Geobacillus%20zalihae.pdf |