Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia
During 2011–2012, 15 bacterial isolates were obtained from wilting banana plants from seven locations in Malaysia. Characterisation of the Malaysian isolates was determined by biovar determination, pathogenicity test, phylotype-specific multiplex PCR (Pmx-PCR) and endoglucanase (egl) gene amplificat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/34911/ http://psasir.upm.edu.my/id/eprint/34911/1/Phylotype%20classification%20of%20Ralstonia%20solanacearum%20biovar%201%20strains%20isolated%20from%20banana%20%28Musa%20spp%29%20in%20Malaysia.pdf |
| _version_ | 1848847906188034048 |
|---|---|
| author | Mohamed Zulperi, Dzarifah Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Awang, Yahya Mohd Hata, Erneeza |
| author_facet | Mohamed Zulperi, Dzarifah Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Awang, Yahya Mohd Hata, Erneeza |
| author_sort | Mohamed Zulperi, Dzarifah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | During 2011–2012, 15 bacterial isolates were obtained from wilting banana plants from seven locations in Malaysia. Characterisation of the Malaysian isolates was determined by biovar determination, pathogenicity test, phylotype-specific multiplex PCR (Pmx-PCR) and endoglucanase (egl) gene amplification. Based on the genotype, phenotype and pathogenic characteristics, all isolates were identified as Ralstonia solanacearum. Pmx- and egl-PCRs indicated that all isolates belong to phylotype II of Ralstonia species complex hierarchical classification. The neighbour joining phylogenetic tree of egl sequences also verified the results where the isolates were all clustered into phylotype II, together with the reference sequences strains, UW070 and UW162. Therefore, the results of our study may provide a better understanding on the taxonomy of R. solanacearum species occupying banana plantations in Malaysia. This study is indeed the first report of phylotype II classification of R. solanacearum biovar 1 strains isolated from banana plants in Malaysia. |
| first_indexed | 2025-11-15T09:26:03Z |
| format | Article |
| id | upm-34911 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:26:03Z |
| publishDate | 2014 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-349112016-05-05T00:47:48Z http://psasir.upm.edu.my/id/eprint/34911/ Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia Mohamed Zulperi, Dzarifah Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Awang, Yahya Mohd Hata, Erneeza During 2011–2012, 15 bacterial isolates were obtained from wilting banana plants from seven locations in Malaysia. Characterisation of the Malaysian isolates was determined by biovar determination, pathogenicity test, phylotype-specific multiplex PCR (Pmx-PCR) and endoglucanase (egl) gene amplification. Based on the genotype, phenotype and pathogenic characteristics, all isolates were identified as Ralstonia solanacearum. Pmx- and egl-PCRs indicated that all isolates belong to phylotype II of Ralstonia species complex hierarchical classification. The neighbour joining phylogenetic tree of egl sequences also verified the results where the isolates were all clustered into phylotype II, together with the reference sequences strains, UW070 and UW162. Therefore, the results of our study may provide a better understanding on the taxonomy of R. solanacearum species occupying banana plantations in Malaysia. This study is indeed the first report of phylotype II classification of R. solanacearum biovar 1 strains isolated from banana plants in Malaysia. Taylor & Francis 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34911/1/Phylotype%20classification%20of%20Ralstonia%20solanacearum%20biovar%201%20strains%20isolated%20from%20banana%20%28Musa%20spp%29%20in%20Malaysia.pdf Mohamed Zulperi, Dzarifah and Sijam, Kamaruzaman and Mior Ahmad, Zainal Abidin and Awang, Yahya and Mohd Hata, Erneeza (2014) Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia. Archives of Phytopathology and Plant Protection, 47 (19). pp. 2352-2364. ISSN 0323-5408; ESSN: 1477-2906 http://www.tandfonline.com/doi/abs/10.1080/03235408.2013.876748 10.1080/03235408.2013.876748 |
| spellingShingle | Mohamed Zulperi, Dzarifah Sijam, Kamaruzaman Mior Ahmad, Zainal Abidin Awang, Yahya Mohd Hata, Erneeza Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia |
| title | Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia |
| title_full | Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia |
| title_fullStr | Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia |
| title_full_unstemmed | Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia |
| title_short | Phylotype classification of Ralstonia solanacearum biovar 1 strains isolated from banana (Musa spp) in Malaysia |
| title_sort | phylotype classification of ralstonia solanacearum biovar 1 strains isolated from banana (musa spp) in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/34911/ http://psasir.upm.edu.my/id/eprint/34911/ http://psasir.upm.edu.my/id/eprint/34911/ http://psasir.upm.edu.my/id/eprint/34911/1/Phylotype%20classification%20of%20Ralstonia%20solanacearum%20biovar%201%20strains%20isolated%20from%20banana%20%28Musa%20spp%29%20in%20Malaysia.pdf |