PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae
Perfluorooctane sulfonate (PFOS) is a widely spread environmental contaminant. It accumulates in the brain and has potential neurotoxic effects. The exposure to PFOS has been associated with higher impulsivity and increased ADHD prevalence. We investigated the effects of developmental exposure to PF...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34892/ http://psasir.upm.edu.my/id/eprint/34892/1/PFOS%20induces%20behavioral%20alterations.pdf |
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| author | Spulber , Stefan Kilian, Pascal Wan Ibrahim, Wan Norhamidah Onishchenko, Natalia Ulhaq, Mazhar Norrgren, Leif Negri, Sara Di Tuccio, Marcello Ceccatelli, Sandra |
| author_facet | Spulber , Stefan Kilian, Pascal Wan Ibrahim, Wan Norhamidah Onishchenko, Natalia Ulhaq, Mazhar Norrgren, Leif Negri, Sara Di Tuccio, Marcello Ceccatelli, Sandra |
| author_sort | Spulber , Stefan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Perfluorooctane sulfonate (PFOS) is a widely spread environmental contaminant. It accumulates in the brain and has potential neurotoxic effects. The exposure to PFOS has been associated with higher impulsivity and increased ADHD prevalence. We investigated the effects of developmental exposure to PFOS in zebrafish larvae, focusing on the modulation of activity by the dopaminergic system. We exposed zebrafish embryos to 0.1 or 1 mg/L PFOS (0.186 or 1.858 µM, respectively) and assessed swimming activity at 6 dpf. We analyzed the structure of spontaneous activity, the hyperactivity and the habituation during a brief dark period (visual motor response), and the vibrational startle response. The findings in zebrafish larvae were compared with historical data from 3 months old male mice exposed to 0.3 or 3 mg/kg/day PFOS throughout gestation. Finally, we investigated the effects of dexamfetamine on the alterations in spontaneous activity and startle response in zebrafish larvae. We found that zebrafish larvae exposed to 0.1 mg/L PFOS habituate faster than controls during a dark pulse, while the larvae exposed to 1 mg/L PFOS display a disorganized pattern of spontaneous activity and persistent hyperactivity. Similarly, mice exposed to 0.3 mg/kg/day PFOS habituated faster than controls to a new environment, while mice exposed to 3 mg/kg/day PFOS displayed more intense and disorganized spontaneous activity. Dexamfetamine partly corrected the hyperactive phenotype in zebrafish larvae. In conclusion, developmental exposure to PFOS in zebrafish induces spontaneous hyperactivity mediated by a dopaminergic deficit, which can be partially reversed by dexamfetamine in zebrafish larvae. |
| first_indexed | 2025-11-15T09:25:57Z |
| format | Article |
| id | upm-34892 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:25:57Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-348922016-01-26T03:13:11Z http://psasir.upm.edu.my/id/eprint/34892/ PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae Spulber , Stefan Kilian, Pascal Wan Ibrahim, Wan Norhamidah Onishchenko, Natalia Ulhaq, Mazhar Norrgren, Leif Negri, Sara Di Tuccio, Marcello Ceccatelli, Sandra Perfluorooctane sulfonate (PFOS) is a widely spread environmental contaminant. It accumulates in the brain and has potential neurotoxic effects. The exposure to PFOS has been associated with higher impulsivity and increased ADHD prevalence. We investigated the effects of developmental exposure to PFOS in zebrafish larvae, focusing on the modulation of activity by the dopaminergic system. We exposed zebrafish embryos to 0.1 or 1 mg/L PFOS (0.186 or 1.858 µM, respectively) and assessed swimming activity at 6 dpf. We analyzed the structure of spontaneous activity, the hyperactivity and the habituation during a brief dark period (visual motor response), and the vibrational startle response. The findings in zebrafish larvae were compared with historical data from 3 months old male mice exposed to 0.3 or 3 mg/kg/day PFOS throughout gestation. Finally, we investigated the effects of dexamfetamine on the alterations in spontaneous activity and startle response in zebrafish larvae. We found that zebrafish larvae exposed to 0.1 mg/L PFOS habituate faster than controls during a dark pulse, while the larvae exposed to 1 mg/L PFOS display a disorganized pattern of spontaneous activity and persistent hyperactivity. Similarly, mice exposed to 0.3 mg/kg/day PFOS habituated faster than controls to a new environment, while mice exposed to 3 mg/kg/day PFOS displayed more intense and disorganized spontaneous activity. Dexamfetamine partly corrected the hyperactive phenotype in zebrafish larvae. In conclusion, developmental exposure to PFOS in zebrafish induces spontaneous hyperactivity mediated by a dopaminergic deficit, which can be partially reversed by dexamfetamine in zebrafish larvae. Public Library of Science 2014-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34892/1/PFOS%20induces%20behavioral%20alterations.pdf Spulber , Stefan and Kilian, Pascal and Wan Ibrahim, Wan Norhamidah and Onishchenko, Natalia and Ulhaq, Mazhar and Norrgren, Leif and Negri, Sara and Di Tuccio, Marcello and Ceccatelli, Sandra (2014) PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae. PLOS ONE, 9 (4). art. no. e94227. pp. 1-13. ISSN 1932-6203 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0094227 10.1371/journal.pone.0094227 |
| spellingShingle | Spulber , Stefan Kilian, Pascal Wan Ibrahim, Wan Norhamidah Onishchenko, Natalia Ulhaq, Mazhar Norrgren, Leif Negri, Sara Di Tuccio, Marcello Ceccatelli, Sandra PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae |
| title | PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae |
| title_full | PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae |
| title_fullStr | PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae |
| title_full_unstemmed | PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae |
| title_short | PFOS induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae |
| title_sort | pfos induces behavioral alterations, including spontaneous hyperactivity that is corrected by dexamfetamine in zebrafish larvae |
| url | http://psasir.upm.edu.my/id/eprint/34892/ http://psasir.upm.edu.my/id/eprint/34892/ http://psasir.upm.edu.my/id/eprint/34892/ http://psasir.upm.edu.my/id/eprint/34892/1/PFOS%20induces%20behavioral%20alterations.pdf |