Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system
Background: Thyroid autoantibodies are measured in the evaluation of patients with thyroid diseases. The clinically important thyroid autoantibodies are anti-thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG). The aim of this study was to assess the analytical performance of anti-TPO and...
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| Format: | Article |
| Language: | English |
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Community Health Society Malaysia
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34873/ http://psasir.upm.edu.my/id/eprint/34873/1/Performance%20characteristics%20of%20anti.pdf |
| _version_ | 1848847895274455040 |
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| author | Samsudin, Intan Nureslyna Chellappah Thambiah, Subashini P., Hannah B., Nor Baizurah Mohd Hussain, Baizurah |
| author_facet | Samsudin, Intan Nureslyna Chellappah Thambiah, Subashini P., Hannah B., Nor Baizurah Mohd Hussain, Baizurah |
| author_sort | Samsudin, Intan Nureslyna |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Thyroid autoantibodies are measured in the evaluation of patients with thyroid diseases. The clinically important thyroid autoantibodies are anti-thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG). The aim of this study was to assess the analytical performance of anti-TPO and anti-TG assays on Roche Cobas e411, an automated electrochemiluminescence immunoassay (ECLIA) system.
Materials and Methods: The following parameters, imprecision, accuracy and stability were evaluated. Imprecision (within-run and between-run) and accuracy studies were performed using two levels of quality control materials. The stability of samples for anti-TPO and anti-TG measurements were evaluated by calculating the percentage recovery of serum samples stored at different temperatures (4°C, -20°C and -80°C) on day 3 and day 12 of storage.
Result: The imprecisions for anti-TPO were between 2.5 to 11.3%. The percentage deviation from the true value was -4.9% and -3.6% for low and high QC, respectively. Anti-TPO showed variable recovery from 92.0% to 119.0% on day 3 and day 12 of storage. For anti-TG, the imprecisions were between 3.0 to 11.5%. The percentage deviation from the true value was 3.5% and 12.7% for low and high QC, respectively. Anti-TG showed variable recovery from 89.8% to 117.0% on day 3 and day 12 of storage.
Conclusion: Roche Cobas e411 anti-TPO and anti-TG assays have demonstrated acceptable precision and accuracy. It is recommended that samples for anti-TPO and anti-TG are stored and analysed as per recommended to avoid the effects of storage. |
| first_indexed | 2025-11-15T09:25:52Z |
| format | Article |
| id | upm-34873 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:25:52Z |
| publishDate | 2014 |
| publisher | Community Health Society Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-348732016-01-27T02:42:58Z http://psasir.upm.edu.my/id/eprint/34873/ Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system Samsudin, Intan Nureslyna Chellappah Thambiah, Subashini P., Hannah B., Nor Baizurah Mohd Hussain, Baizurah Background: Thyroid autoantibodies are measured in the evaluation of patients with thyroid diseases. The clinically important thyroid autoantibodies are anti-thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG). The aim of this study was to assess the analytical performance of anti-TPO and anti-TG assays on Roche Cobas e411, an automated electrochemiluminescence immunoassay (ECLIA) system. Materials and Methods: The following parameters, imprecision, accuracy and stability were evaluated. Imprecision (within-run and between-run) and accuracy studies were performed using two levels of quality control materials. The stability of samples for anti-TPO and anti-TG measurements were evaluated by calculating the percentage recovery of serum samples stored at different temperatures (4°C, -20°C and -80°C) on day 3 and day 12 of storage. Result: The imprecisions for anti-TPO were between 2.5 to 11.3%. The percentage deviation from the true value was -4.9% and -3.6% for low and high QC, respectively. Anti-TPO showed variable recovery from 92.0% to 119.0% on day 3 and day 12 of storage. For anti-TG, the imprecisions were between 3.0 to 11.5%. The percentage deviation from the true value was 3.5% and 12.7% for low and high QC, respectively. Anti-TG showed variable recovery from 89.8% to 117.0% on day 3 and day 12 of storage. Conclusion: Roche Cobas e411 anti-TPO and anti-TG assays have demonstrated acceptable precision and accuracy. It is recommended that samples for anti-TPO and anti-TG are stored and analysed as per recommended to avoid the effects of storage. Community Health Society Malaysia 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34873/1/Performance%20characteristics%20of%20anti.pdf Samsudin, Intan Nureslyna and Chellappah Thambiah, Subashini and P., Hannah and B., Nor Baizurah and Mohd Hussain, Baizurah (2014) Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system. International Journal of Public Health and Clinical Sciences, 1 (2). pp. 51-57. ISSN 2289-7577 http://publichealthmy.org/ejournal/ojs2/index.php/ijphcs/article/view/118 |
| spellingShingle | Samsudin, Intan Nureslyna Chellappah Thambiah, Subashini P., Hannah B., Nor Baizurah Mohd Hussain, Baizurah Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system |
| title | Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system |
| title_full | Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system |
| title_fullStr | Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system |
| title_full_unstemmed | Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system |
| title_short | Performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas E411 immunoassay system |
| title_sort | performance characteristics of anti-thyroid peroxidase and anti-thyroglobulin assays on roche cobas e411 immunoassay system |
| url | http://psasir.upm.edu.my/id/eprint/34873/ http://psasir.upm.edu.my/id/eprint/34873/ http://psasir.upm.edu.my/id/eprint/34873/1/Performance%20characteristics%20of%20anti.pdf |