p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model
Studies with Diethylstilbestrol (DES) in humans and rodents have resulted in a spectrum of toxic and carcinogenic effects. Previous findings on gene expression profiles following DES treatment showed that p53, p21 and bax was transcriptionally regulated in this model. In the present studies, we used...
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IDOSI Publications
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34803/ http://psasir.upm.edu.my/id/eprint/34803/1/p53%20and%20p21%20mRNA%20and%20protein%20expression%20in%20treated%20synthetic%20oestrogen%20in%20mouse%20transgenic%20animal%20model.pdf |
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| author | Salleh, Mohd Nazil Ahmad Mulyadi Lai, Henkie Isahwan Mustaffa, Mohd Irfan Raimi Wan Ramli, Wan Khairuzzaman Ismail, Patimah |
| author_facet | Salleh, Mohd Nazil Ahmad Mulyadi Lai, Henkie Isahwan Mustaffa, Mohd Irfan Raimi Wan Ramli, Wan Khairuzzaman Ismail, Patimah |
| author_sort | Salleh, Mohd Nazil |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Studies with Diethylstilbestrol (DES) in humans and rodents have resulted in a spectrum of toxic and carcinogenic effects. Previous findings on gene expression profiles following DES treatment showed that p53, p21 and bax was transcriptionally regulated in this model. In the present studies, we used Reverse Transcriptase in situ Polymerase Chain Reaction (RT in situ PCR) and immunohistochemistry techniques for localisation and expression of p53, p21 and bax at cellular levels. Animal were housed individually and treated with 500μmole/kg b.w of DES, (ip) once daily up to four days. Our results have shown, the expression of p53, p21 and bax mRNA were greater in wild-type compared to p53+/- knockout mice. In addition, p53, p21 and bax mRNA were significantly high in DES- treated compared to control-vehicle animals. Collectively, similar patterns of
expression also were seen in p53 and p21 proteins and scored according to the percentage of positive nuclear
staining. Therefore, the combination of p53 and p21 were concluded to be a good prognostic marker for development of carcinogenesis. |
| first_indexed | 2025-11-15T09:25:34Z |
| format | Article |
| id | upm-34803 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:25:34Z |
| publishDate | 2014 |
| publisher | IDOSI Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-348032016-01-27T08:20:07Z http://psasir.upm.edu.my/id/eprint/34803/ p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model Salleh, Mohd Nazil Ahmad Mulyadi Lai, Henkie Isahwan Mustaffa, Mohd Irfan Raimi Wan Ramli, Wan Khairuzzaman Ismail, Patimah Studies with Diethylstilbestrol (DES) in humans and rodents have resulted in a spectrum of toxic and carcinogenic effects. Previous findings on gene expression profiles following DES treatment showed that p53, p21 and bax was transcriptionally regulated in this model. In the present studies, we used Reverse Transcriptase in situ Polymerase Chain Reaction (RT in situ PCR) and immunohistochemistry techniques for localisation and expression of p53, p21 and bax at cellular levels. Animal were housed individually and treated with 500μmole/kg b.w of DES, (ip) once daily up to four days. Our results have shown, the expression of p53, p21 and bax mRNA were greater in wild-type compared to p53+/- knockout mice. In addition, p53, p21 and bax mRNA were significantly high in DES- treated compared to control-vehicle animals. Collectively, similar patterns of expression also were seen in p53 and p21 proteins and scored according to the percentage of positive nuclear staining. Therefore, the combination of p53 and p21 were concluded to be a good prognostic marker for development of carcinogenesis. IDOSI Publications 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34803/1/p53%20and%20p21%20mRNA%20and%20protein%20expression%20in%20treated%20synthetic%20oestrogen%20in%20mouse%20transgenic%20animal%20model.pdf Salleh, Mohd Nazil and Ahmad Mulyadi Lai, Henkie Isahwan and Mustaffa, Mohd Irfan Raimi and Wan Ramli, Wan Khairuzzaman and Ismail, Patimah (2014) p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model. Academic Journal of Cancer Research, 7 (2). pp. 109-116. ISSN 1995-8943; ESSN: 2221-3422 http://www.idosi.org/ajcr/ajcr7%282%2914.htm 10.5829/idosi.ajcr.2014.7.2.8384 |
| spellingShingle | Salleh, Mohd Nazil Ahmad Mulyadi Lai, Henkie Isahwan Mustaffa, Mohd Irfan Raimi Wan Ramli, Wan Khairuzzaman Ismail, Patimah p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model |
| title | p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model |
| title_full | p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model |
| title_fullStr | p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model |
| title_full_unstemmed | p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model |
| title_short | p53 and p21 mRNA and protein expression in treated synthetic oestrogen in mouse transgenic animal model |
| title_sort | p53 and p21 mrna and protein expression in treated synthetic oestrogen in mouse transgenic animal model |
| url | http://psasir.upm.edu.my/id/eprint/34803/ http://psasir.upm.edu.my/id/eprint/34803/ http://psasir.upm.edu.my/id/eprint/34803/ http://psasir.upm.edu.my/id/eprint/34803/1/p53%20and%20p21%20mRNA%20and%20protein%20expression%20in%20treated%20synthetic%20oestrogen%20in%20mouse%20transgenic%20animal%20model.pdf |