Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology
Background Extraction prior to component analysis is the primary step in the recovery and isolation of bioactive phytochemicals from plant materials. Methods Response surface methodology was applied to optimize ultrasound-assisted extraction conditions followed by ultra high performance liquid ch...
| Main Authors: | , , , |
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| Format: | Article |
| Published: |
BioMed Central
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34785/ |
| _version_ | 1848847871005163520 |
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| author | Ghasemzadeh, Ali Jaafar, Hawa Z. E. Karimi, Ehsan Rahmat, Asmah |
| author_facet | Ghasemzadeh, Ali Jaafar, Hawa Z. E. Karimi, Ehsan Rahmat, Asmah |
| author_sort | Ghasemzadeh, Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background
Extraction prior to component analysis is the primary step in the recovery and isolation of bioactive phytochemicals from plant materials.
Methods
Response surface methodology was applied to optimize ultrasound-assisted extraction conditions followed by ultra high performance liquid chromatography (UHPLC) to achieve high catechin, myricetin, and quercetin contents, and high antioxidant and anticancer activities in the curry leaf extracts. The antioxidant and anticancer activities of the leaf extracts were determined by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, respectively. The central composite experimental design (3-level, 3-factorial) was employed to consider the effects of ultrasonic power (80–150 W), temperature (40–80°C), and methanol dilution (40–80%) on the properties of the curry leaf extracts.
Results
It was found that ultrasonic power of 145.49 W at 55.9°C with 80% methanol was the most appropriate set of conditions for the extraction of catechin, myricetin, and quercetin from curry leaves with the consequent high antioxidant activity. Using the optimum extraction conditions, the extraction yields of catechin, myricetin, and quercetin were 0.482, 0.517, and 0.394 mg/g DW, respectively, and the antioxidant activity was enhanced to 83%. The optimized extract showed more distinct anticancer activity against HeLa cancer cells in a concentration of 67.2 μg/mL (P < 0.01) without toxicity to normal cells.
Conclusions
The results indicated that the pharmaceutical quality of curry leaves could be improved significantly by optimizing the extraction process using response surface methodology.
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| first_indexed | 2025-11-15T09:25:29Z |
| format | Article |
| id | upm-34785 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T09:25:29Z |
| publishDate | 2014 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-347852015-12-23T01:38:32Z http://psasir.upm.edu.my/id/eprint/34785/ Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology Ghasemzadeh, Ali Jaafar, Hawa Z. E. Karimi, Ehsan Rahmat, Asmah Background Extraction prior to component analysis is the primary step in the recovery and isolation of bioactive phytochemicals from plant materials. Methods Response surface methodology was applied to optimize ultrasound-assisted extraction conditions followed by ultra high performance liquid chromatography (UHPLC) to achieve high catechin, myricetin, and quercetin contents, and high antioxidant and anticancer activities in the curry leaf extracts. The antioxidant and anticancer activities of the leaf extracts were determined by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, respectively. The central composite experimental design (3-level, 3-factorial) was employed to consider the effects of ultrasonic power (80–150 W), temperature (40–80°C), and methanol dilution (40–80%) on the properties of the curry leaf extracts. Results It was found that ultrasonic power of 145.49 W at 55.9°C with 80% methanol was the most appropriate set of conditions for the extraction of catechin, myricetin, and quercetin from curry leaves with the consequent high antioxidant activity. Using the optimum extraction conditions, the extraction yields of catechin, myricetin, and quercetin were 0.482, 0.517, and 0.394 mg/g DW, respectively, and the antioxidant activity was enhanced to 83%. The optimized extract showed more distinct anticancer activity against HeLa cancer cells in a concentration of 67.2 μg/mL (P < 0.01) without toxicity to normal cells. Conclusions The results indicated that the pharmaceutical quality of curry leaves could be improved significantly by optimizing the extraction process using response surface methodology. BioMed Central 2014-12 Article PeerReviewed Ghasemzadeh, Ali and Jaafar, Hawa Z. E. and Karimi, Ehsan and Rahmat, Asmah (2014) Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology. BMC Complementary and Alternative Medicine, 14 (1). art. no. 318. pp. 1-10. ISSN 1472-6882 http://link.springer.com/article/10.1186%2F1472-6882-14-318 10.1186/1472-6882-14-318 |
| spellingShingle | Ghasemzadeh, Ali Jaafar, Hawa Z. E. Karimi, Ehsan Rahmat, Asmah Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology |
| title | Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology |
| title_full | Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology |
| title_fullStr | Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology |
| title_full_unstemmed | Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology |
| title_short | Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology |
| title_sort | optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (murraya koenigii l.) using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/34785/ http://psasir.upm.edu.my/id/eprint/34785/ http://psasir.upm.edu.my/id/eprint/34785/ |