Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology
This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β-galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://psasir.upm.edu.my/id/eprint/34777/ http://psasir.upm.edu.my/id/eprint/34777/1/34777.pdf |
| _version_ | 1848847868649013248 |
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| author | Abdul Khalil, Khalilah Mustafa, Shuhaimi Mohamad, Rosfarizan Ariff, Arbakariya Shaari, Yamin Abdul Manap, Yazid Ahmad, Siti Aqlima Dahalan, Farrah Aini |
| author_facet | Abdul Khalil, Khalilah Mustafa, Shuhaimi Mohamad, Rosfarizan Ariff, Arbakariya Shaari, Yamin Abdul Manap, Yazid Ahmad, Siti Aqlima Dahalan, Farrah Aini |
| author_sort | Abdul Khalil, Khalilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β-galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 23 full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk and yeast extract showed significant influence on the biomass production and, based on the steepest ascent step, middle points of skim milk (6% wt/vol) and yeast extract (1.89% wt/vol) were obtained. A polynomial regression model in FCCD-RSM revealed that both factors were found significant and the strongest influence was given by skim milk concentration. Optimum concentrations of skim milk and yeast extract for maximum biomass G4 and β-galactosidase production meanwhile low in lactose and FAN balance after cultivation period were 5.89% (wt/vol) and 2.31% (wt/vol), respectively. The validation experiments showed that the predicted and experimental values are not significantly different, indicating that the FCCD-RSM model developed is sufficient to describe the cultivation process of G4 using skim-milk-based medium with the addition of yeast extract. |
| first_indexed | 2025-11-15T09:25:27Z |
| format | Article |
| id | upm-34777 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:25:27Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-347772016-10-10T04:18:49Z http://psasir.upm.edu.my/id/eprint/34777/ Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology Abdul Khalil, Khalilah Mustafa, Shuhaimi Mohamad, Rosfarizan Ariff, Arbakariya Shaari, Yamin Abdul Manap, Yazid Ahmad, Siti Aqlima Dahalan, Farrah Aini This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β-galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 23 full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk and yeast extract showed significant influence on the biomass production and, based on the steepest ascent step, middle points of skim milk (6% wt/vol) and yeast extract (1.89% wt/vol) were obtained. A polynomial regression model in FCCD-RSM revealed that both factors were found significant and the strongest influence was given by skim milk concentration. Optimum concentrations of skim milk and yeast extract for maximum biomass G4 and β-galactosidase production meanwhile low in lactose and FAN balance after cultivation period were 5.89% (wt/vol) and 2.31% (wt/vol), respectively. The validation experiments showed that the predicted and experimental values are not significantly different, indicating that the FCCD-RSM model developed is sufficient to describe the cultivation process of G4 using skim-milk-based medium with the addition of yeast extract. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34777/1/34777.pdf Abdul Khalil, Khalilah and Mustafa, Shuhaimi and Mohamad, Rosfarizan and Ariff, Arbakariya and Shaari, Yamin and Abdul Manap, Yazid and Ahmad, Siti Aqlima and Dahalan, Farrah Aini (2014) Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology. BioMed Research International, 2014. art. no. 787989. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2014/787989/abs/ 10.1155/2014/787989 |
| spellingShingle | Abdul Khalil, Khalilah Mustafa, Shuhaimi Mohamad, Rosfarizan Ariff, Arbakariya Shaari, Yamin Abdul Manap, Yazid Ahmad, Siti Aqlima Dahalan, Farrah Aini Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology |
| title | Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology |
| title_full | Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology |
| title_fullStr | Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology |
| title_full_unstemmed | Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology |
| title_short | Optimization of milk-based medium for efficient cultivation of Bifidobacterium pseudocatenulatum G4 using face-centered central composite-response surface methodology |
| title_sort | optimization of milk-based medium for efficient cultivation of bifidobacterium pseudocatenulatum g4 using face-centered central composite-response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/34777/ http://psasir.upm.edu.my/id/eprint/34777/ http://psasir.upm.edu.my/id/eprint/34777/ http://psasir.upm.edu.my/id/eprint/34777/1/34777.pdf |