Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435
The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
A & V Publications
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/34770/ http://psasir.upm.edu.my/id/eprint/34770/1/Optimization%20of%20enzymatic%20synthesis%20of%203-O-%CE%B2-D-glucopyranoside%20betulinic%20acid%20by%20Novozyme-435.pdf |
| _version_ | 1848847866526695424 |
|---|---|
| author | Abdu, Hamisu Ahmad @ Amat, Faujan Basri, Mahiran Ismail, Intan Safinar Abdul Rahman, Mohd Basyaruddin |
| author_facet | Abdu, Hamisu Ahmad @ Amat, Faujan Basri, Mahiran Ismail, Intan Safinar Abdul Rahman, Mohd Basyaruddin |
| author_sort | Abdu, Hamisu |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69 % and the predicted value was 89.05 %. |
| first_indexed | 2025-11-15T09:25:25Z |
| format | Article |
| id | upm-34770 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T09:25:25Z |
| publishDate | 2014 |
| publisher | A & V Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-347702018-10-09T03:07:43Z http://psasir.upm.edu.my/id/eprint/34770/ Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435 Abdu, Hamisu Ahmad @ Amat, Faujan Basri, Mahiran Ismail, Intan Safinar Abdul Rahman, Mohd Basyaruddin The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69 % and the predicted value was 89.05 %. A & V Publications 2014-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/34770/1/Optimization%20of%20enzymatic%20synthesis%20of%203-O-%CE%B2-D-glucopyranoside%20betulinic%20acid%20by%20Novozyme-435.pdf Abdu, Hamisu and Ahmad @ Amat, Faujan and Basri, Mahiran and Ismail, Intan Safinar and Abdul Rahman, Mohd Basyaruddin (2014) Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435. Asian Journal of Research in Chemistry, 7 (7). pp. 640-643. ISSN 0974-4169; ESSN: 0974-4150 http://ajrconline.org/AbstractView.aspx?PID=2014-7-7-6 |
| spellingShingle | Abdu, Hamisu Ahmad @ Amat, Faujan Basri, Mahiran Ismail, Intan Safinar Abdul Rahman, Mohd Basyaruddin Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435 |
| title | Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435 |
| title_full | Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435 |
| title_fullStr | Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435 |
| title_full_unstemmed | Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435 |
| title_short | Optimization of enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid by Novozyme-435 |
| title_sort | optimization of enzymatic synthesis of 3-o-β-d-glucopyranoside betulinic acid by novozyme-435 |
| url | http://psasir.upm.edu.my/id/eprint/34770/ http://psasir.upm.edu.my/id/eprint/34770/ http://psasir.upm.edu.my/id/eprint/34770/1/Optimization%20of%20enzymatic%20synthesis%20of%203-O-%CE%B2-D-glucopyranoside%20betulinic%20acid%20by%20Novozyme-435.pdf |